Asian-Australasian Journal of Animal Sciences

Asian Australasian Association of Animal Production Societies (아세아태평양축산학회)
권호 표지
DOI QR코드

DOI QR Code

Polymorphism of Insulin-like Growth Factor Binding Protein-4 Gene in 17 Pig Breeds and Its Relationship with Growth Traits

  • Wang, Wenjun (State Key Laboratory for Agrobiotechnology, China Agricultural University) ;
  • Hu, Xiaoxiang (State Key Laboratory for Agrobiotechnology, China Agricultural University) ;
  • Fei, Jin (State Key Laboratory for Agrobiotechnology, China Agricultural University) ;
  • Meng, Qinyong (State Key Laboratory for Agrobiotechnology, China Agricultural University) ;
  • Li, Ning (State Key Laboratory for Agrobiotechnology, China Agricultural University)
  • Received : 2006.09.11
  • Accepted : 2007.01.04
  • Published : 2007.10.01
Download PDF
⟨ Previous Next ⟩

Abstract

Insulin-like growth factor binding protein-4 (IGFBP-4) is a member of the IGF super family, and regulates the action of IGFs. The polymorphism of porcine IGFBP-4 gene in 17 pig breeds (total n = 570) was detected by PCR-SSCP, and alleles A and B were detected. In these pig breeds, it was found that exotic pig breeds carried high frequencies of allele A, while Chinese native pig breeds carried high frequencies of allele B. The role of porcine IGFBP-4 was investigated in 172 F2 offspring of a $Lantang{\times}Lantang $ population. Forty eight growth traits were recorded for analyzing the association between IGFBP-4 gene polymorphism and quantitative performance traits. In this resource family, pigs with AA genotype had higher fore-body weight, bone weight of mid-body, bone weight of rear-body, fore-leg weight and rear-leg weight than those pigs with BB genotype (p<0.05); while pigs which carried BB genotype had higher back-fat thickness at C point and lard weight than those pigs with AA genotype (p<0.05); pigs with AA genotype had higher body weight than those with BB genotype; for meat quality traits, pigs with AA genotype had higher meat color than those of BB genotype (p<0.01), and pigs with BB genotype had higher marbling than those of AA and AB genotypes (p<0.01 and p<0.05, respectively).Based on these results, it is necessary to do more studies on IGFBP-4 before using the IGFBP-4 locus for the application of marker-assisted selection programs.

Keywords

References

  1. Allander, S. V., S. Bajalica, C. Larsson, H. Luthman, D. R. Powell, I. Stern, G. Weber, H. Zazzi and E. Ehrenborg. 1993. Structure and chromosomal localization of human insulin-like growth factor-binding protein genes. Growth. Regul. 3:3-5.
  2. Clapper, J. A., T. M. Clark and L. A. Rempel. 2000. Serum concentration of IGF-I, estradiol-17$\beta$, testosterone, and relative amounts of IGF-binding proteins (IGFBP) in growing boars, barrows, and gilts. J. Anim. Sci. 78:2581-2588.
  3. Cohick, W. S., A. Gockerman and D. R. Clemmons. 1993. Vascular smooth muscle cells synthesize two forms of insulinlike growth factor binding proteins which are regulated differently by the insulin-like growth factors. J. Cell. Physiol. 157:52-60. https://doi.org/10.1002/jcp.1041570107
  4. Gerrard, D. E., C. S. Okamura and A. L. Grant. 1999. Expression and location of IGF binding proteins-2, -4, and -5 in developing fetal tissue. J. Anim. Sci. 77:1431-1441. https://doi.org/10.2527/1999.7761431x
  5. Harrell, R. J., M. J. Thomas, R. D. Boyd, S. M. Czerwinski, N. C. Steele and C. Bauman. 1999. Ontogenic maturation of the somatotropin/insulin-like growth factor axis. J. Anim. Sci. 77:2934-2941. https://doi.org/10.2527/1999.77112934x
  6. Hathaway, M. R., W. R. Dayton, M. E. White and M. S. Pampush. 2003. Effects of antimicrobials and weaning on porcine serum insulin-like growth factor binding protein levels. J. Anim. Sci. 81:1456-1463.
  7. Jones, J. I. and D. R. Clemmons. 1995 Insulin-like growth factors and their binding proteins: Biological actions. Endocr. Rev. 16:3-34.
  8. Kelly, K. M., Y. Oh, S. E. Gargosky, Z. Gucev, T. Matsumoto, V. Hwa, L. Ng, D. M. Simpson and R. G. Rosenfeld. 1996. Insulin-like growth factor-binding proteins (IGFBPs) and their regulatory dynamics. Int. J. Biochem. Cell. Biol. 28:619-637. https://doi.org/10.1016/1357-2725(96)00005-2
  9. Lawrence, A. Wetterau, Michael G. Moore, Kuk-Wah Lee, Melanie L. Shim and Pinchas Cohen. 1999. Novel Aspects of the Insulin-like Growth Factor Binding Proteins. Molecul. Genet. Metab. 68:161-181. https://doi.org/10.1006/mgme.1999.2920
  10. Le Roith, D., C. Bondy, S. Yakar, J. L. Liu and A. Butler. 2001. The somatomedin hypothesis: 2001. Endocr. Rev. 22:53-74. https://doi.org/10.1210/er.22.1.53
  11. Li Jiaqi. 2001.QTL mapping and analysis of candidate gene for important economic traits in swine. Dissertation for Doctoral Degree. SouthChina Agricultural University, Guangzhou, Guangdong, P. R. China (in Chinese)
  12. Malek, M., J. C. M. Dekkers, H. K. Lee, T. J. Baas and M. F. Rothschild. 2001a. A molecular genome scan analysis to identify chromosomal regions influencing economic traits in the pig. I. Growth and body composition. Mamm. Genome. 12:630-636. https://doi.org/10.1007/s003350020018
  13. Malek, M., J. C. M. Dekkers, H. K. Lee, T. J. Baas and M. F. Rothschild. 2001b. A molecular genome scan analysis to identify chromosomal regions influencing economic traits in the pig. I. Growth and body composition. Mamm. Genome. 12:637-645. https://doi.org/10.1007/s003350020019
  14. Mohan, S., C. M. Bautista, J. Wergedal and D. J. Baylink. 1989. Isolation of an inhibitory insulin-like growth factor (IGF) binding protein from bone cell-conditioned medium: a potential local regulator of IGF action. Proc. Natl. Acad. Sci. USA. 86:8338-8342. https://doi.org/10.1073/pnas.86.21.8338
  15. Muller, E., G. Moser, H. Bartenschlager and H. Geldermann. 2000. Trait values of growth, carcass and meat quality in Wild Boar, Meishan and Pietrain pigs as well as their crossbred generations. J. Anim. Breed. Genet. 117:189-202. https://doi.org/10.1046/j.1439-0388.2000.00239.x
  16. Paszek, A. A., P. J. Wilkie, G. H. Flickinger, G. A. Rohrer, L. J. Alexander, C. W. Beattie and L. B. Schook. 1999. Interval mapping of growth in divergent swine cross. Mamm. Genome. 10:117-122. https://doi.org/10.1007/s003359900955
  17. Paszek, A. A., P. J. Wilkie, G. H. Flickinger, G. A. Rohrer, L. J. Alexander, C. W. Beattie and L. B. Schook. 1999. Interval mapping of growth in divergent swine cross. Mamm. Genome. 10:117-122. https://doi.org/10.1007/s003359900955
  18. Rechler, M. M. 1993. Insulin-like growth factor binding proteins. Vitam. Horm. 47:1-114. https://doi.org/10.1016/S0083-6729(08)60444-6
  19. SAS. 1989. SAS User's Guide. SAS Inst. Inc., Cary, NC.
  20. Shimasaki, S. and N. Ling. 1991. Identification and molecular characterization of insulin-like growth factor binding proteins (IGFBP-1, -2, -3, -4, -5 and -6). Prog. Growth. Factor. Res. 3:243-266. https://doi.org/10.1016/0955-2235(91)90003-M
  21. Shimonaka, M., R. Schroder, S. Shimasakai and N. Ling. 1989. Identification of a novel binding protein for insulin-like growth factors in adult serum. Biochem. Biophys. Res. Commun. 165:1890-1895.
  22. Wang, J., W. Niu, D. P. Witte, S. D. Chernausek, Y. E. Nikiforox, T. L. Clemens, B. Sharifi, A. R. Strauch and J. A. Fagin. 1998. Overexpression of insulin-like growth factor-binding protein-4 (IGFBP-4) in smooth muscle cells of transgenic mice through a smooth muscle alpha-actin-IGFBP-4 fusion gene induces smooth muscle hypoplasia. Endocrinol. 139:2605-2614. https://doi.org/10.1210/en.139.5.2605
  23. Wang,, W. J., Y. H. Zhao, Y. H. Zhang and N. Li. 2005. Assignment of porcine insulin-like growth factor-binding protein-4 (IGFBP4) to SSC12 by fluorescence in situ hybridization and radiation hybrid mapping. Anim. Genet. 36: 522-523. https://doi.org/10.1111/j.1365-2052.2005.01361.x
  24. Wang Wenjun, Huang Lusheng, Gao Jun, Chen Kefei and Ren Jun. 2003. Polymorphism of growth hormone gene in 12 pig breeds and its relationship with growth and carcass traits. Asian-Aust. J. Anim. Sci. 16:161-164. https://doi.org/10.5713/ajas.2003.161
  25. Wenjun Wang, Kehui Ouyang, Xifan Su, Mingsheng Xu and Xinchen Shangguan. 2006. Polymorphism of Insulin-like Growth Factor 1 Receptor Gene in 12 Pig Breeds and Its Relationship with Pig Performance Traits. Asian-Aust. J. Anim. Sci. 19:1541-1545. https://doi.org/10.5713/ajas.2006.1541
  26. Yue, G., J. R. J. Schröffel, G. Moser, H. Bartenschlager, G. Reiner and H. Geldermann. 2003. Linkage and QTL mapping for Sus scrofa chromosome 12. J. Anim. Breed. Genet. 120 (Suppl. 1) 95-102.
  27. Zazzi, H., A. Nikoshkov, K. Hall and H. Luthman. 1998. Structure and transcription regulation of the human insulin-like growth factor binding protein 4 gene (IGFBP4). Genomics. 49:401-410 https://doi.org/10.1006/geno.1998.5283