Browse > Article
http://dx.doi.org/10.5713/ajas.2007.1490

Polymorphism of Insulin-like Growth Factor Binding Protein-4 Gene in 17 Pig Breeds and Its Relationship with Growth Traits  

Wang, Wenjun (State Key Laboratory for Agrobiotechnology, China Agricultural University)
Hu, Xiaoxiang (State Key Laboratory for Agrobiotechnology, China Agricultural University)
Fei, Jin (State Key Laboratory for Agrobiotechnology, China Agricultural University)
Meng, Qinyong (State Key Laboratory for Agrobiotechnology, China Agricultural University)
Li, Ning (State Key Laboratory for Agrobiotechnology, China Agricultural University)
Publication Information
Asian-Australasian Journal of Animal Sciences / v.20, no.10, 2007 , pp. 1490-1495 More about this Journal
Abstract
Insulin-like growth factor binding protein-4 (IGFBP-4) is a member of the IGF super family, and regulates the action of IGFs. The polymorphism of porcine IGFBP-4 gene in 17 pig breeds (total n = 570) was detected by PCR-SSCP, and alleles A and B were detected. In these pig breeds, it was found that exotic pig breeds carried high frequencies of allele A, while Chinese native pig breeds carried high frequencies of allele B. The role of porcine IGFBP-4 was investigated in 172 F2 offspring of a $Lantang{\times}Lantang $ population. Forty eight growth traits were recorded for analyzing the association between IGFBP-4 gene polymorphism and quantitative performance traits. In this resource family, pigs with AA genotype had higher fore-body weight, bone weight of mid-body, bone weight of rear-body, fore-leg weight and rear-leg weight than those pigs with BB genotype (p<0.05); while pigs which carried BB genotype had higher back-fat thickness at C point and lard weight than those pigs with AA genotype (p<0.05); pigs with AA genotype had higher body weight than those with BB genotype; for meat quality traits, pigs with AA genotype had higher meat color than those of BB genotype (p<0.01), and pigs with BB genotype had higher marbling than those of AA and AB genotypes (p<0.01 and p<0.05, respectively).Based on these results, it is necessary to do more studies on IGFBP-4 before using the IGFBP-4 locus for the application of marker-assisted selection programs.
Keywords
IGFBP-4 Gene; Performance Traits; Swine; PCR-SSCP;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
Times Cited By Web Of Science : 0  (Related Records In Web of Science)
Times Cited By SCOPUS : 0
연도 인용수 순위
1 Yue, G., J. R. J. Schröffel, G. Moser, H. Bartenschlager, G. Reiner and H. Geldermann. 2003. Linkage and QTL mapping for Sus scrofa chromosome 12. J. Anim. Breed. Genet. 120 (Suppl. 1) 95-102.
2 Zazzi, H., A. Nikoshkov, K. Hall and H. Luthman. 1998. Structure and transcription regulation of the human insulin-like growth factor binding protein 4 gene (IGFBP4). Genomics. 49:401-410   DOI   ScienceOn
3 Kelly, K. M., Y. Oh, S. E. Gargosky, Z. Gucev, T. Matsumoto, V. Hwa, L. Ng, D. M. Simpson and R. G. Rosenfeld. 1996. Insulin-like growth factor-binding proteins (IGFBPs) and their regulatory dynamics. Int. J. Biochem. Cell. Biol. 28:619-637.   DOI   ScienceOn
4 Lawrence, A. Wetterau, Michael G. Moore, Kuk-Wah Lee, Melanie L. Shim and Pinchas Cohen. 1999. Novel Aspects of the Insulin-like Growth Factor Binding Proteins. Molecul. Genet. Metab. 68:161-181.   DOI   ScienceOn
5 Hathaway, M. R., W. R. Dayton, M. E. White and M. S. Pampush. 2003. Effects of antimicrobials and weaning on porcine serum insulin-like growth factor binding protein levels. J. Anim. Sci. 81:1456-1463.
6 Jones, J. I. and D. R. Clemmons. 1995 Insulin-like growth factors and their binding proteins: Biological actions. Endocr. Rev. 16:3-34.
7 Malek, M., J. C. M. Dekkers, H. K. Lee, T. J. Baas and M. F. Rothschild. 2001b. A molecular genome scan analysis to identify chromosomal regions influencing economic traits in the pig. I. Growth and body composition. Mamm. Genome. 12:637-645.   DOI   ScienceOn
8 Mohan, S., C. M. Bautista, J. Wergedal and D. J. Baylink. 1989. Isolation of an inhibitory insulin-like growth factor (IGF) binding protein from bone cell-conditioned medium: a potential local regulator of IGF action. Proc. Natl. Acad. Sci. USA. 86:8338-8342.   DOI   ScienceOn
9 Muller, E., G. Moser, H. Bartenschlager and H. Geldermann. 2000. Trait values of growth, carcass and meat quality in Wild Boar, Meishan and Pietrain pigs as well as their crossbred generations. J. Anim. Breed. Genet. 117:189-202.   DOI   ScienceOn
10 Gerrard, D. E., C. S. Okamura and A. L. Grant. 1999. Expression and location of IGF binding proteins-2, -4, and -5 in developing fetal tissue. J. Anim. Sci. 77:1431-1441.   DOI
11 Le Roith, D., C. Bondy, S. Yakar, J. L. Liu and A. Butler. 2001. The somatomedin hypothesis: 2001. Endocr. Rev. 22:53-74.   DOI   ScienceOn
12 Cohick, W. S., A. Gockerman and D. R. Clemmons. 1993. Vascular smooth muscle cells synthesize two forms of insulinlike growth factor binding proteins which are regulated differently by the insulin-like growth factors. J. Cell. Physiol. 157:52-60.   DOI   ScienceOn
13 Wang, J., W. Niu, D. P. Witte, S. D. Chernausek, Y. E. Nikiforox, T. L. Clemens, B. Sharifi, A. R. Strauch and J. A. Fagin. 1998. Overexpression of insulin-like growth factor-binding protein-4 (IGFBP-4) in smooth muscle cells of transgenic mice through a smooth muscle alpha-actin-IGFBP-4 fusion gene induces smooth muscle hypoplasia. Endocrinol. 139:2605-2614.   DOI   ScienceOn
14 Paszek, A. A., P. J. Wilkie, G. H. Flickinger, G. A. Rohrer, L. J. Alexander, C. W. Beattie and L. B. Schook. 1999. Interval mapping of growth in divergent swine cross. Mamm. Genome. 10:117-122.   DOI
15 Paszek, A. A., P. J. Wilkie, G. H. Flickinger, G. A. Rohrer, L. J. Alexander, C. W. Beattie and L. B. Schook. 1999. Interval mapping of growth in divergent swine cross. Mamm. Genome. 10:117-122.   DOI
16 Rechler, M. M. 1993. Insulin-like growth factor binding proteins. Vitam. Horm. 47:1-114.   DOI
17 Li Jiaqi. 2001.QTL mapping and analysis of candidate gene for important economic traits in swine. Dissertation for Doctoral Degree. SouthChina Agricultural University, Guangzhou, Guangdong, P. R. China (in Chinese)
18 Malek, M., J. C. M. Dekkers, H. K. Lee, T. J. Baas and M. F. Rothschild. 2001a. A molecular genome scan analysis to identify chromosomal regions influencing economic traits in the pig. I. Growth and body composition. Mamm. Genome. 12:630-636.   DOI   ScienceOn
19 Wang,, W. J., Y. H. Zhao, Y. H. Zhang and N. Li. 2005. Assignment of porcine insulin-like growth factor-binding protein-4 (IGFBP4) to SSC12 by fluorescence in situ hybridization and radiation hybrid mapping. Anim. Genet. 36: 522-523.   DOI   ScienceOn
20 Wang Wenjun, Huang Lusheng, Gao Jun, Chen Kefei and Ren Jun. 2003. Polymorphism of growth hormone gene in 12 pig breeds and its relationship with growth and carcass traits. Asian-Aust. J. Anim. Sci. 16:161-164.   과학기술학회마을   DOI
21 SAS. 1989. SAS User's Guide. SAS Inst. Inc., Cary, NC.
22 Shimasaki, S. and N. Ling. 1991. Identification and molecular characterization of insulin-like growth factor binding proteins (IGFBP-1, -2, -3, -4, -5 and -6). Prog. Growth. Factor. Res. 3:243-266.   DOI   ScienceOn
23 Shimonaka, M., R. Schroder, S. Shimasakai and N. Ling. 1989. Identification of a novel binding protein for insulin-like growth factors in adult serum. Biochem. Biophys. Res. Commun. 165:1890-1895.
24 Wenjun Wang, Kehui Ouyang, Xifan Su, Mingsheng Xu and Xinchen Shangguan. 2006. Polymorphism of Insulin-like Growth Factor 1 Receptor Gene in 12 Pig Breeds and Its Relationship with Pig Performance Traits. Asian-Aust. J. Anim. Sci. 19:1541-1545.   과학기술학회마을   DOI
25 Clapper, J. A., T. M. Clark and L. A. Rempel. 2000. Serum concentration of IGF-I, estradiol-17$\beta$, testosterone, and relative amounts of IGF-binding proteins (IGFBP) in growing boars, barrows, and gilts. J. Anim. Sci. 78:2581-2588.
26 Harrell, R. J., M. J. Thomas, R. D. Boyd, S. M. Czerwinski, N. C. Steele and C. Bauman. 1999. Ontogenic maturation of the somatotropin/insulin-like growth factor axis. J. Anim. Sci. 77:2934-2941.   DOI
27 Allander, S. V., S. Bajalica, C. Larsson, H. Luthman, D. R. Powell, I. Stern, G. Weber, H. Zazzi and E. Ehrenborg. 1993. Structure and chromosomal localization of human insulin-like growth factor-binding protein genes. Growth. Regul. 3:3-5.