Archives of Plastic Surgery

Korean Society of Plastic and Reconstructive Surgeons (대한성형외과학회)
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Difference of Gene Expression in Venous Malformation

정맥기형 환자에 있어서의 유전자 발현 차이

  • Kim, Taek Kyun (Department of Plastic and Reconstructive Surgery, School of Medicine, Kyungpook National University) ;
  • Oh, Eun Jung (Department of Advanced Material Engineering, School of Engineering, Kyungpook National University) ;
  • Cho, Byung Chae (Department of Plastic and Reconstructive Surgery, School of Medicine, Kyungpook National University) ;
  • Chung, Ho Yun (Department of Plastic and Reconstructive Surgery, School of Medicine, Kyungpook National University)
  • 김택균 (경북대학교 의과대학 성형외과학교실) ;
  • 오은정 (경북대학교 공과대학 물질기능공학과) ;
  • 조병채 (경북대학교 의과대학 성형외과학교실) ;
  • 정호윤 (경북대학교 의과대학 성형외과학교실)
  • Received : 2007.06.25
  • Published : 2007.11.10
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Abstract

Purpose: Venous malformation(VM) which often causes pain and discomfort is the most common type of vascular malformations. Although it is presented with disfigured appearance and associated soft tissue or skeletal hypertrophy, the molecular bases of VMs are poorly understood. Differentially expressed genes(DEGs) of VMs were investigated to illuminate the molecular mechanism of the disease entity. Methods: Gene expressions of VM patients' subcutaneous tissue were studied in comparison with normal persons' by $GeneFishing^{TM}$ technique using the annealing control primers (ACPs) to identify DEGs. Candidate genes were sequenced and screened by basic local alignment search tool (BLAST) afterwards. Results: Among seventy DEGs identified, forty DEGs which had shown significantly different expression pattern were sequenced. Twenty eight out of 40 were up-regulated while 12 were down-regulated. BLAST searches revealed that 37 were known genes and 3 were unknown genes. Many genes were involved in the differentiation and remodeling of smooth muscle cells, opposed to the previous hypothesis that a lot of angiogenetic genes would be involved. Furthermore, several transcription factors and related genes, as well as cell signaling and metabolism regulators, were up regulated. Conclusion: It suggests that analysis of DEGs in VMs provide basic knowledge about its pathophysiology. and new therapeutic approaches.

Keywords

Acknowledgement

Supported by : 경북대학교병원

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