Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Two Threonine Residues Required for Role of AfsKav in Controlling Morphogenesis and Avermectin Production in Streptomyces avermitilis

  • Rajkarnikar, Arishma (Department of Biological Science, Institute of Bioscience and Biotechnology, Myongji University) ;
  • Kwon, Hyung-Jin (Department of Biological Science, Institute of Bioscience and Biotechnology, Myongji University) ;
  • Ryu, Yeon-Woo (Department of Molecular Science and Technology, Ajou University) ;
  • Suh, Joo-Won (Department of Biological Science, Institute of Bioscience and Biotechnology, Myongji University)
  • Published : 2007.09.30
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Abstract

AfsKav is a eukaryotic-type serine/threonine protein kinase, required for sporulation and avermectin production in Streptomyces avermitilis. In terms of their ability to complement SJW4001 (${\Delta}afsK$-av), afsK-av mutants T165A and T168A were not functional, whereas mutants T165D and T168D retained their ability, indicating that Thr-165 and Thr-168 are the phosphorylation sites required for the role of AfsKav. Expression of the S-adenosylmethione synthetase gene promoted avermectin production in the wild-type S. avermitilis, yet not in the mutant harboring T168D or T165D, demonstrating that tandem phosphorylation on Thr-165 and Thr-168 in AfsKav is the mechanism modulating avermectin production in response to S-adenosylmethione accumulation in S. avermitilis.

Keywords

References

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