Journal of the Korean Association of Oral and Maxillofacial Surgeons

The Korean Association of Oral and Maxillofacial Surgeons (대한구강악안면외과학회)
권호 표지

AN EXPERIMENTAL STUDY FOR THE DETECTION OF AUTOCRINE GROWTH ACTIVITY IN THE OSTEOGENIC CELLS AFTER MANDIBULAR DISTRACTION OSTEOGENESIS

하악골 신장술 후 신생골 조직에서 자가분비성장능력의 활성에 대한 실험적 연구

  • Byun, June-Ho (Department of Oral & Maxillofacial Surgery, School of Medicine and Institute of Health Sciences, Gyeongsang National University) ;
  • Park, Bong-Wook (Department of Oral & Maxillofacial Surgery, School of Medicine and Institute of Health Sciences, Gyeongsang National University) ;
  • Park, Seong-Cheol (Department of Oral & Maxillofacial Surgery, School of Medicine and Institute of Health Sciences, Gyeongsang National University) ;
  • Kim, Gyoo-Cheon (Department of Oral Anatomy, School of Dentistry, Pusan National University) ;
  • Park, Bong-Soo (Department of Oral Anatomy, School of Dentistry, Pusan National University) ;
  • Kim, Jong-Ryoul (Department of Oral & Maxillofacial Surgery, School of Dentistry, Pusan National University)
  • 변준호 (경상대학교 의학전문대학원 치과학교실 구강악안면외과, 경상대학교 건강과학연구원) ;
  • 박봉욱 (경상대학교 의학전문대학원 치과학교실 구강악안면외과, 경상대학교 건강과학연구원) ;
  • 박성철 (경상대학교 의학전문대학원 치과학교실 구강악안면외과, 경상대학교 건강과학연구원) ;
  • 김규천 (부산대학교 치의학전문대학원 구강해부학교실) ;
  • 박봉수 (부산대학교 치의학전문대학원 구강해부학교실) ;
  • 김종렬 (부산대학교 치의학전문대학원 구강악안면외과학교실)
  • Published : 2007.08.31
Download PDF
⟨ Previous Next ⟩

Abstract

Background: Distraction osteogenesis(DO) is a useful method for treating cases demanding the generation of new bone. During DO, the angiogenic activity is crucial factor in the new bone formation. The aim of this study was to detect the autocrine growth activity in the cellular components of the distracted bone with observation of the co-expression of vascular endothelial growth factor(VEGF) and its receptors following the mandibular DO. Materials and methods: Unilateral mandibular distraction(0.5 mm twice per day for 10 days) was performed in six mongrel dogs. Two animals were killed at 7, 14, and 28 days after completion of distraction, respectively. Immediately after the animals were killed, the right mandibles were harvested en block. Immunohistochemical staining was processed for observation of the VEGF expression, and double immunofluorescent staining was also processed for detection of the co-expression of osteocalcin and VEGF's two distinct receptors(VEGFR-1 and VEGFR-2). Results: At 7 and 14 days after distraction, the expressions of VEGF were significantly increased in the osteogenic cells of the distracted bone. Up to 28 days after distraction, VEGF was still expressed moderate in the osteoblastic cells of distracted bone. The co-expressions of osteocalcin/VEGFR-1 and osteocalcin/VEGFR-2 were observed in the distracted bone at 7 and 14 days after distraction. In the double immunofluorescent staining, the co-expression' s level of osteocalcin/VEGFR-1 was more than that of osteocalcin/VEGFR-2. Conclusion: Taken together, this study suggested that VEGF plays an important role in the osteogenesis, and these osteoblastic cell-derived VEGF might act as autocrine growth factor during distraction osteogenesis. In the other word, the cellular components, such as osteoblasts and immature fibroblast-like cellsor mesenchymal cells in the distracted bone, might have autocrine growth activity during distraction osteogenesis.

Keywords

References

  1. Tavakoli T, Yu Y, Shahidi S, Bonar F, Walsh WR, Poole MD: Expression of growth factors in the mandibular distraction zone: a sheep study. Br J Plast Surg 1999;52:434-439 https://doi.org/10.1054/bjps.1999.3157
  2. Farhadieh RD, Dickson R, Yu Y, Gianoutsos MP, Walsh WG: The role of transtorming growth factor-beta, insulin-like growth factor I, and basic fibroblast growth factor in distraction osteogenesis of the mandible. J Craniofac Surg 1999;10:80-86 https://doi.org/10.1097/00001665-199901000-00016
  3. Bouletreau PJ, Warren SM, Longaker MT: The molecular biology of distraction osteogenesis. J Cranio-Maxillofac Surg 2002;30:1-11 https://doi.org/10.1054/jcms.2001.0263
  4. Byun JH, Park BW, Sung IY, Cho YC, Kim JR: Immunohistochemical study of osteopontin expression in the distracted bone after canine mandibular distraction osteogenesis. J Kor Oral Maxillofac Surg 2006;32:418-425
  5. Hwang DU, Byun JH, Park BW, Kim JH: Expression of vascular endothelial growth factor and its receptors in the distracted periosteum after mandibular distraction osteogenesis. J Kor Maxillofac Plast Reconstr Surg 2006;28:549-558
  6. Glowacki J: Angiogenesis in fracture repair. Clin Orthop Relat Res 1998;355S:82-89 https://doi.org/10.1097/00003086-199810001-00010
  7. Li G, Simpson AH, Kenwright J, Triffitt JT: Effect of lengthening rate on angiogenesis during distraction osteogenesis. J Orthop Res 1999;17:362-367 https://doi.org/10.1002/jor.1100170310
  8. Kurdy NMG, Weiss JB, Bate A: Endothelial stimulating angiogenic factor in early fracture healing. Injury 1996;27:143-145 https://doi.org/10.1016/0020-1383(95)00169-7
  9. Harada S, Rodan SB, Rodan GA: Expression and regulation of vascular endothelial growth factor in osteoblasts. Clin Orthop Relat Res 1995;313:76-80
  10. Goad D, Rubin J, Wang H, Tashjian AH Jr, Patterson C: Enhanced expression of vascular endothelial growth factor in human SaOS-2 osteoblast-like cells and murine osteoblast induced by insulin-like growth factor I. Endocrinology 1996;137:2262-2268 https://doi.org/10.1210/en.137.6.2262
  11. Hu J, Zou S, Li J, Chen Y, Wang D, Gao Z: Temporospatial expression of vascular endothelial growth factor and basic fibroblast growth factor duringmandibular distraction osteogenesis. J Cranio-Maxillofac Surg 2003;31:238-243 https://doi.org/10.1016/S1010-5182(03)00034-9
  12. Neufeld G, Cohen T, Gengrinovitch S, Poltorak Z: Vascular endothelial growth factor (VEGF) and its receptors. FASEB 1999;13:9-22 https://doi.org/10.1096/fasebj.13.1.9
  13. Hansen-Algenstaedt N, Algenstaedt P, Bottcher A, Joscheck C, Schwarzlon B, Schaefer C, Muller I, Koike C, Ruther W, Fink B: Bilaterally increase VEGF-levels in muscle during experimental unilateral callus distraction. J Orthop Res 2003;21:805-812 https://doi.org/10.1016/S0736-0266(03)00060-3
  14. Decker MM, Karperien M, van der Bent C, Yamashita T, Papapoulos SE, Lowik CW: Expression of vascular endothelial growth factors and their receptors during osteoblastic differentiation. Endocrinology 2000;141:1667-1674 https://doi.org/10.1210/en.141.5.1667
  15. Mayr-Wohlfart U, Waltenberger J, Hausser H, Kessler S, Gunther KP, Dehio C, Paul W, Brenner RE: Vascular endothelial growth factor stimulates chemotatic migration of primary human osteoblasts. Bone 2002;30:472-477 https://doi.org/10.1016/S8756-3282(01)00690-1
  16. Fiedler J, Leucht F, Waltenberger J, Dehio C, Brenner RE: VEGF-A and PIGF-1 stimulate chemotactic migration of human mesenchymal progenitor cells. Biochem Biophys Res Commun 2005;334:561-568 https://doi.org/10.1016/j.bbrc.2005.06.116
  17. Mayer H, Bertram H, Lindenmaier W, Korff T, Weber H, Weich H: Vascular endothelial growth factor (VEGF-A) expression in human mesenchymal stem cells: autocrine and paracrine role on osteoblastic and endothelial differentiation. J Cell Biochem 2005;95:827-839 https://doi.org/10.1002/jcb.20462
  18. Park BW, Kim JR, Lee JH, Byun JH: Expression of nerve growth factor and vascular endothelial growth factor in the inferior alveolar nerve after distraction osteogenesis. Int J Oral Maxillofac Surg 2006;35:624-630 https://doi.org/10.1016/j.ijom.2006.02.019
  19. Trueta J: The role of the vessels in osteogenesis. J Bone Joint Surg Br 1963;45:402-418
  20. Reilly TM, Seldes R, Luchetti W, Brighton CT: Similarities in the phenotypic expression of percytes and bone cells. Clin Orthop 1998;346:95-103
  21. Paciccia DM, Patel N, Lee C, Salisbury K, Lehmann W, Carvalho R, Gerstenfeld LC, Einhorn TA: Expression of angiogenic factors during distraction osteogenesis. Bone 2003;33:889-898 https://doi.org/10.1016/j.bone.2003.06.002
  22. Warren SM, Mehrara BJ, Steinbrench DS, Paccione MF, Greenwald JA, Spector JA, Longaker MT: Rat mandibular distraction osteogenesis: Part III. Gradual distraction versus acute lengthening. Plast Recontr Surg 2001;107:441-453 https://doi.org/10.1097/00006534-200102000-00021
  23. Steinbrech DS, Mehrara BJ, Saadeh PB, Greenwald JA, Spector JA, Gittes GK, Longaker MT: VEGF expression in an osteoblast-like cell line is regulated by a hypoxia response mechanism. Am J Physiol Cell Physiol 2000;278:C853-860
  24. Spector JA, Mehrara BJ, Greenwald JA, Saadeh PB, Steinbrech DS, Bouletreau PJ, Smith LP, Longaker MT: Osteoblast expression of vascular endothelial growth factor is modulated by the extracellular microenvironment. Am J Physiol Cell Physiol 2000;280:72-80
  25. Street J, Winter D, Wang JH, Wakai A, McGuinness A, Redmond HP: Is human fracture hematoma inherently angiogenic? Clin Orthop 2000;378:224-234 https://doi.org/10.1097/00003086-200009000-00033
  26. Furumatsu T, Shen ZN, Kawai A, Nishida K, Manabe H, Oohashi T, Inoune H, Ninomiya Y: Vascular endothelial growth factor principally acts as the main angiogenesis factor in the early stage of human osteoblastogenesis. J Biochem 2003;133:633-639 https://doi.org/10.1093/jb/mvg081
  27. Villars F, Bordenave L, Bareille R, Amedee J: Effect of human endothelial cell on human bone marrow stromal cell phenotype: role of VEGF? J Cell Biochem 2000;79:672-685 https://doi.org/10.1002/1097-4644(20001215)79:4<672::AID-JCB150>3.0.CO;2-2
  28. Richard M, Goulet JA, Schaffler MB, Goldstein SA: Temporal and spatial characterization of regenerate bone in the lengthened rabbit tibia. J Bone Miner Res 1999;14:1978-1986 https://doi.org/10.1359/jbmr.1999.14.11.1978