한국양식학회지 (Journal of Aquaculture)

한국양식학회 (The Korean Society of Fisheries and Aquatic Science)
권호 표지

Osmoregulation and mRNA Expression of a Heat Shock Protein 68 and Glucose-regulated Protein 78 in the Pacific oyster Crassostrea gigas in Response to Salinity Changes

  • Jo, Pil-Gue (Division of Marine Environment and Bioscience, Korea Maritime University) ;
  • Choi, Yong-Ki (Division of Marine Environment and Bioscience, Korea Maritime University) ;
  • An, Kwang-Wook (Division of Marine Environment and Bioscience, Korea Maritime University) ;
  • Choi, Cheol-Young (Division of Marine Environment and Bioscience, Korea Maritime University)
  • 발행 : 2007.12.31
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초록

Stress-inducible proteins may function in part as molecular chaperones, protecting cells from damage due to various stresses and helping to maintain homeostasis. We examined the mRNA expression patterns of a 68-kDa heat shock protein (HSP68) and 78-kDa glucose-regulated protein (GRP78) in relation to physiological changes in Pacific oyster Crassostrea gigas under osmotic stress. Expression of HSP68 and GRP78 mRNA in the gill significantly increased until 48 h in a hypersaline environment (HRE) and 72 h in a hyposaline environment (HOE), and then decreased. Osmolality and the concentrations of $Na^+$, $Cl^-$, and $Ca^{2+}$ in the hemolymph of HRE oysters significantly increased until 72 h (the highest value) and then gradually decreased; in HOE oysters, these values significantly decreased until 72 h (the lowest value), and then increased. These results suggest that osmolality and $Na^+$, $Cl^-$, and $Ca^{2+}$ concentrations were stabilized by HSP68 and GRP78, and indicate that these two stress-induced proteins play an important role in regulating the metabolism and protecting the cells of the Pacific oysters exposed to salinity changes.

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