Applied Microscopy

Korean Society of Microscopy (한국현미경학회)
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Morphological Study of the Regeneration of the Mouse Olfactory Epithelial Cells after Destruction by Intranasal Zinc Sulfate Irrigation

코 안 $ZnSO_4$ 점적으로 손상된 마우스 후각 상피세포의 재생에 대한 형태학적 연구

  • Kang, Wha-Sun (Department of Natural Science, College of Medicine, Catholic University) ;
  • Moon, Young-Wha (Department of Natural Science, College of Medicine, Catholic University)
  • 강화선 (가톨릭대학교 의과대학 자연과학교실) ;
  • 문영화 (가톨릭대학교 의과대학 자연과학교실)
  • Published : 2007.12.31
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Abstract

The morphological effects of intranasal zinc sulfate(5% solution) irrigation on the mouse olfactory epithelium and the regeneration process of olfactory receptor cells following nasal irrigation were studied with scanning and transmission electron microscope. The results were as follows: 1. The septal epithelium except some basal cells was wholly detached from the basement membrane, during the first 6 to 24 hours after 5% zinc sulfate irrigation. 2. 3 days after $ZnSO_4$ treatment, two layered septal epithelium was formed from basal cells. And microvilli were observed in the apical epithelium of newly formed olfactory epithelial cells. 3. 5 days after treatment, a lot of centrosomes and basal bodies were observed in the olfactory receptor cells, and cilia were lined up between microvilli on the apical membrane of olfactory receptor cells. And immature olfactory knob was first observed in the newly formed olfactory receptor cells. Mature olfactory knob was observed 1 week after treatment. 4. There are very many mature olfactory knobs in the olfactory receptor cells 2 weeks after intranasal zinc sulfate irrigation. These results support that treatment with 5% zinc sulfate is a good experimental model for the regeneration of mammalian nervous tissues because this method could thoroughly detach the septal epithelium. During the regeneration of olfactory receptor cells, the surface membrane of the olfactory receptor cells widen the surface with the microvilli. Then cilia, which arranged in a line, substituted for the microvilli. The part of the surface membrane with cilia protruded and finally formed the olfactory vesicle.

마우스의 코 안으로 5% $ZnSO_4$용액을 점적하는 것이 코중격 후각상피에 미치는 영향과, 그 이후 후각수용세포가 재생되는 과정을 주사전자현미경과 투과전자현미경을 사용하여 형태학적으로 조사하였고, 그 결과는 다음과 같다. 1. 5% $ZnSO_4$용액을 점적한 후 6시간에서 24시간 사이에 코중격 후각상피층은 일부의 기저세포들을 제외하고는 완전하게 탈락되었다. 2. $ZnSO_4$처리 후 3일군에서는 코중격 후각상피세포들 중 부분적으로 남아 있던 기저세포들의 세포분열로 형성된 새로운 후각상피세포들로 후각상피층은 2층으로 나타났다. 또한 새롭게 형성된 후각상피세포들의 상부 세포막에는 미세응모가 돌출되었다. 3. 5% $ZnSO_4$용액을 점적하고 5일이 경과된 코중격 후각수용세포들에서는 다수의 중심소체와 기저소체가 관찰되었고, 상부 세포막에는 미세응모들 사이에 섬모들이 줄지어 나타났다. 또한 새롭게 형성된 코중격 후각수용세포들에서 처음으로 후각소포의 초기 형태가 나타났으며, 1주가 경과된 후각상피층에는 전형적인 형태의 후각소포를 관찰할 수 있었다. 4. 5% $ZnSO_4$용액 점적 후 2주가 경과된 경우, 성숙한 형태의 후각소포를 가지고 있는 후각수용세포가 관찰되었다. 이와 같은 결과는 5% $ZnSO_4$용액의 처리가 마우스 코중격의 후각상피층을 완전하게 박리시킬 수 있으므로 포유류 신경조직의 재생연구를 위해 유용한 실험적 모델임을 뒷받침한다, 또한 새롭게 재생되는 후각수용세포의 상부 세포막이 처음에는 미세응모로 표면적을 넓히고, 시간이 경과함에 따라 일렬로 배열된 섬모들로 대치되며, 그 후 섬모를 포함한 상부세포막이 부분적으로 돌출하고, 마지막으로 전형적인 후각소포로 발달한다.

Keywords

References

  1. Albert JR, Galef BG: Acute anosmia in the rat: a behavioral test of a peripherally-induced olfactory deficit. Physiol Behav 6: 619-621, 1971 https://doi.org/10.1016/0031-9384(71)90218-6
  2. Astic L, Saucier D: Neuronal plasticity and regeneration in the olfactory system of mammals: morphological and functional recovery following olfactory bulb deafferentation. Cell Mol Life Sci 58: 5538-5545, 2001
  3. Borders AS, Getchell ML, Etscheidt JT, Rooijen NV, Cohen DA, Getchell TV: Macrophage depletion in the murine olfactory epithelium leads to increased neuronal death and decreased neurogenesis. J Comp Neurol 501 : 206-218, 2007 https://doi.org/10.1002/cne.21252
  4. Burd GD: Morphological study of the effects of intranasal zinc sulfate irrigation on the mouse olfactory epithelium and olfactory bulb. Microscopy Res & Tec 24 : 195-213, 1993 https://doi.org/10.1002/jemt.1070240302
  5. Calof AL, Chikaraishi DM: Analysis of neurogenesis in a mammalian neuroepithelium: Proliferation and differentiation of an olfactory neuron precursors in vitro. Neuron 3 : 115-127,1989 https://doi.org/10.1016/0896-6273(89)90120-7
  6. Cancalon P: Degeneration and regeneration of olfactory cells induced by $ZnSO_4$ and other chemicals. Tiss & Cell 14(4) : 717-733, 1982 https://doi.org/10.1016/0040-8166(82)90061-1
  7. Carggiano M, Kauer JS, Hunter DD: Globose basal cells are the neuronal progenitors in the olfactory epithelium: a lineage analysis using a replication incompetent retrovirus. Neuron 13: 339-352, 1994 https://doi.org/10.1016/0896-6273(94)90351-4
  8. Carter LA, MacDonald JL, Roskams AJ: Olfactory horizontal basal cells demonstrate a conserved multipotent progenitor phenotype. J Neuroscience 24(25) : 5670-5683, 2004 https://doi.org/10.1523/JNEUROSCI.0330-04.2004
  9. Cotsarelis G, Cheng SZ, Dong G, Sun TT, Lavker RM: Existence of slow-cyeling limbal epithelial basal cells that can be preferentially stimulated to proliferate: Implications on epithelial stem cells. Cell 57 : 201-209, 1989 https://doi.org/10.1016/0092-8674(89)90958-6
  10. Crews L, Hunter D: Neurogenesis in the olfactory epithelium. Perspect Dev Neurobiol 2: 151-161, 1994
  11. DeHarmer M, Guevara JL, Hanon K, Olwin BB, Calof AL: Genesis of olfactory receptor neurons in vitro: regulation of progenitor cell division by fibroblast growth factors. Neuron 13 : 1083-1097, 1994 https://doi.org/10.1016/0896-6273(94)90047-7
  12. Ducray A, Bondier JR, Michel G, Bon K, Propper A, Kastner A: Recovery following peripheral destruction of olfactory neurons in young and adult mice. Eur J Neuroscience 15 : 1907-1917, 2002 https://doi.org/10.1046/j.1460-9568.2002.02044.x
  13. Farbman AI: Olfactory neurogenesis: Genetic or environmental control? Trends Neurosci 13 : 362-365, 1990 https://doi.org/10.1016/0166-2236(90)90017-5
  14. Graziadei PPC, Monti Graziadei GA: Neurogenesis and neuron regeneration in the olfactory system of mammals. I. Morphological aspects of differentiation and structural organization of the olfactory sensory neurons. J Neurocytol 8 : 1-18, 1979 https://doi.org/10.1007/BF01206454
  15. Graziadei PP, Monti Graziadei AG: Regeneration in the olfactory system of vertebrates. [Review] Am J Otolaryngol 4 : 228-233, 1983 https://doi.org/10.1016/S0196-0709(83)80063-5
  16. Harding JW, Getchell TV, Margolis FL: Denervation of the primary olfactory pathway in mice. V. Long-term effect of intranasal $ZnSO_4$ irrigation on behavior, biochemistry and morphology. Brain Res 140: 271-285, 1978 https://doi.org/10.1016/0006-8993(78)90460-2
  17. Holbrook EH, Mieleszko Szumowski KE, Schwob JE: An immunochemical, ultrastructural, and developmental characterization of the horizontal basal cells of rat olfactory epithelium. J Comp Neurol 363 : 129-146, 1995 https://doi.org/10.1002/cne.903630111
  18. Huard JMT, Schwob JE: Cell cyeles of globose basal cells in rat olfactory epithelium. Dev Dyn 203 : 17-26, 1995 https://doi.org/10.1002/aja.1002030103
  19. Kastner A, Moyse E, Bauer S, Jourdan F, Brun G: Unusual regulation of cyelin D1 and cyelin-dependent kinases cdk2 and cdk4 during in vivo mitotic stimulation of olfactory neuron progenitors in adult mouse. J Neurochem 74: 2343 -2349, 2000 https://doi.org/10.1046/j.1471-4159.2000.0742343.x
  20. Kim MW, Moon YW, Kim BG, Kwak BK, Park YJ: Immunohistological study of the effects of intranasal $ZnSO_4$ instillation on the mouse olfactory epithelium and olfactory bulb. Kor J Otolaryngol 49 : 395-401, 2006. (Korean)
  21. Lewis JL, Dahl AR: Olfactory mucosa: composition, enzymatic localization and metabolism. In: Doty RL, editor. Handbook of olfaction and gustation. New York: Marcel Dekker pp. 33-52, 1995
  22. Mancuso M, Giovanetti A, Brittebo E: Effects of dichlobenil on ultrastructural morphology and cell replication in the mouse olfactory mucosa. Toxicol Pathol 25 : 186-194, 1997 https://doi.org/10.1177/019262339702500208
  23. Matulionis DH: Ultrastructural study of mouse epithelium following destruction by $ZnSO_4$ and its subsequent regeneration. Am J Anat 142 : 67-90, 1975 https://doi.org/10.1002/aja.1001420106
  24. Matulionis DH: Light and electron microscopic study of the degeneration and early regeneration of olfactory epithelium in the mouse. Amer J Anat 145 : 79-100, 1976 https://doi.org/10.1002/aja.1001450106
  25. McBride K, Slotnick B, Margolis FL: Does intranasal application of zinc sulfate produce anosmia in the mouse? An olfactometric and anatomical study. Chem Senses 28: 659-670, 2003 https://doi.org/10.1093/chemse/bjg053
  26. Menco BP: Tight junctional strands first appear in regions where three cells meet in differentiating olfactory epithelium: a freeze-fracture study. J Cell Sci 89 : 495-505, 1988
  27. Morrison EE, Costanzo RM: Scanning electron microscopic study of degeneration and regeneration in the olfactory epithelium after axotomy. J Neurocytol 18 : 393-405, 1989 https://doi.org/10.1007/BF01190842
  28. Schwartz-Levey M, Chikaraishi DM, Kauer JS: Characterization of potential precursor populations in the mouse olfactory epithelium using immunohistochemistry and autoradiography. J Neurosci 11 : 3556-3564, 1991 https://doi.org/10.1523/JNEUROSCI.11-11-03556.1991
  29. Schwob JE, Youngentob SL, Meiri KF: On the formation of neuromata in the peripheral olfactory system. J Comp Neurol 340: 361-380, 1994 https://doi.org/10.1002/cne.903400307
  30. Schwob JE, Youngentob SL, Mezza RC: Reconstitution of the rat olfactory epithelium after methyl bromide-induced lesion. J Comp Nerurol 359 : 15-37, 1995 https://doi.org/10.1002/cne.903590103
  31. Tisdall FF, Brown A, Defries RD: Persistent anosmia following zinc sulfate nasal spraying. J Pediatr 13 : 60-62, 1938 https://doi.org/10.1016/S0022-3476(38)80128-1
  32. Vollrath M, Altmannsberger M, Weber K, Osborn M: An u1tratructural and immunohistological study of the rat olfactory epithelium: Unique properties of olfactory sensory cells. Differentiation 29: 243-253, 1985 https://doi.org/10.1111/j.1432-0436.1985.tb00323.x