한국수산과학회지 (Korean Journal of Fisheries and Aquatic Sciences)

  • 제40권6호
  • /
  • Pages.387-393
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  • 2007
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  • 0374-8111(pISSN)
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  • 2287-8815(eISSN)
한국수산과학회 (The Korean Society of Fisheries and Aquatic Science)
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개다시마 Kiellmaniella crassifolia Miyabe (Phaeophyta)의 암.수배우체 분리 및 보존

Separation and Preservation of the Male and Female Gametophytes of Kjellmaniella crassifolia Miyabe (Phaeophyta)

  • 김진희 (국립수산과학원 동해수산연구소) ;
  • 김영대 (국립수산과학원 동해수산연구소) ;
  • 송홍인 (국립수산과학원 서해수산연구소) ;
  • 공용근 (국립수산과학원 동해수산연구소) ;
  • 이상범 (강릉대학교 해양생명공학부) ;
  • 진형주 (강릉대학교 해양생명공학부)
  • Kim, Jin-Hee (East Sea Regional Fisheries Research Institute, NFRDI) ;
  • Kim, Young-Dae (East Sea Regional Fisheries Research Institute, NFRDI) ;
  • Song, Hong-In (West Sea Regional Fisheries Research institute, NFRDI) ;
  • Gong, Yong-Gun (East Sea Regional Fisheries Research Institute, NFRDI) ;
  • Lee, Sang-Beum (Faculty of Marine Bioscience and Technology, Kangnung National University) ;
  • Jin, Hyung-Joo (Faculty of Marine Bioscience and Technology, Kangnung National University)
  • 발행 : 2007.12.31
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초록

We examined the conditions for separating and preserving the male and female gametophytes of Kjellmaniella crassifolia. The highest percentage of zygote germination (85%) was on semi-solid medium composed of 1.0% transfer gel agar at $15\;^{\circ}C$ and $20\;{\mu}mol/m^2/s$ after a 4-week culture. Zygote germination in PESI liquid medium was 93.5% at $20\;^{\circ}C$ and $20\;{\mu}mol/m^2/s$. The maximum zygote growth was $252{\pm}19.7\;{\mu}m$ on 1.0% transfer gel agar at $15\;^{\circ}C$ and $40\;{\mu}mol/m^2/s$ after 5-week culture, and was $76.7{\pm}2.8\;{\mu}m$ in PESI liquid medium at $20\;^{\circ}C$ and $40\;{\mu}mol/m^2/s$. The respective numbers of separated male and female gametophytes from germinated zygotes were 157 and 93 on 1.0% transfer gel agar and 14 and 28 in PESI liquid medium. The maximum growth of separated male and female gametophytes was $575{\pm}28.3\;{\mu}m$ at $5\;^{\circ}C$ and $60\;{\mu}mol/m^2/s$ and $686{\pm}35.4\;{\mu}m$ at $20\;^{\circ}C$ and $20\;{\mu}mol/m^2/s$ in PESI liquid medium after 3 weeks, respectively. The highest percentage fertilized was $93.3{\pm}5.8%$ at $15\;^{\circ}C$ and $20\;{\mu}mol/m^2/s$ in PESI liquid medium. These results show that the best conditions for the separation and preservation of gametophytes (male and female) consisted of culturing on 1.0% transfer gel agar at $15\;^{\circ}C$ and $20\;{\mu}mol/m^2/s$.

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참고문헌

  1. Ahn, K.H., K.H. Suh and C.S. Oh. 1998. Biosorption of Pb and Cu by Kjellmaniella crassifolia. J. Kor. Environ. Sci. Soc., 7, 653-658
  2. Bae, K.U., J.W. Chang and M.H. Seong. 1977. Studies on the culture of Laminariaceaes. (4) Rapid culture of seedlings of Laminaria japonica Aresch and Laminaria religiosa Miyabe by artificial liquid method. Bull. Fish. Res. Dev. Agency, 17, 151-163
  3. Baik, K.K. 1977. Studies on the seed culture of Laminaria. (1) A process of development and the effect of attachment. Bull. Fish. Res. Dev. Agency, 16, 53-65
  4. Baik, K.K. and C.K. Pyen. 1973. Study on growth of Laminaria japonica ARESTCH in the coastal area of Kang Won Do. Bull. Fish. Res. Dev. Agency, 11, 79-92
  5. Chang, J.W. and S.H. Geon. 1970. Studies on the culture of Laminaria. (1) On the transplantation of tangle Laminaria religiosa Miyabe in temperate zone (the coast of Ilsan-Dong, Ulsan city). Bull. Fish. Res. Dev. Agency, 5, 63-75
  6. Chang, J.W. and D.Y Chung. 1971. Studies on the culture of Laminaria. (2) On the tide over the summer of cultivated Laminaria reiligiosa Miyabe in warm water area. Bull. Fish. Res. Dev. Agency, 8, 31-43
  7. Chang, J.W., D.Y Chung, K.D. Bae and M.N. Yun. 1973. Studies on the culture of Laminaria. (3) Comparison on the growth of cultured Laminaria japonica Aresch in Mipo Bay, Uldan City. Bull. Fish. Res. Dev. Agency, 11, 37-57
  8. Jin, H.J., G.M. Seo, V.C. Cho, E.K. Hwang, C.H. Sohn and YK Hong. 1997. Gelling agents for tissue culture of the seaweed Hizikia fusiformis. J. Appl. Phycol., 9, 489-493
  9. Kang, J.W. 1966. On the geographical distribution of marine algae in Korea, Bull. Busan Fish. Coll., 7, 1-125
  10. Kang, R.S. and C.H. Koh. 1999. Germination and growth of Laminaria japonica (Phaeophyta) microscopic stages under different temperatures and photon irradiances. J. Kor. Fish. Soc., 32, 438-443
  11. Kim, H.G. 2003. Environmental adaptation of native kelp Kjellmaniella crassifolia and marine environment in Gangwon coast. Proceedings of the 6th Korea-Japan Fisheries Seminar, 25-37
  12. Kim, H.G., J.G. Park and D.S. Kim. 2005. Comparative laboratory culture studies of the native kelp Kjellmaniella crassifolia and the introduced kelp Laminaria japonica in east coast of Korea. J. Aguacult., 18, 299-304
  13. Kim, H.G., J.I. Lee, S.B. Lee and J.G. Park. 1999. Distribution and standing crops of Laminaria and Kjellmaniella in the coast of Kangnung Province. Bull. Inst. Environ. Technol., Samchuk Nat. Univ., 1, 153- 158
  14. Kitazume, H. and K. Oishi. 1987. Arsenic distribution in two species Kombu, Laminaria japonica and Kjellmaniella crassiforia from Hakodate. Bull. Fac. Fish., Hokkaido Univ., 38, 156-164
  15. Nam, K.W., C.S. Lee, S.D. Lee, J.W. Chang and Y.C. Kim. 1985. Ecological studies on Laminaria religiosa Miyabe of the coast in Kangw5n Province of Korea. Bull. Fish. Res. Dev. Agency, 36, 89-103
  16. Notoya, M. and H.G. Kim. 1996. Influence of light intensity and temperature on callus cell propagation and differentiation to bladelets from the explants of young sporophyte of Kjellmaniella crassifolia Miyabe (Phaeophyta, Laminariales). Algae, 11, 179-182
  17. Ozski, A., H. Mizuta and H. Yamamoto. 2001. Physiological differences between the nutrient uptakes of Kjellmaniella crassifolia and Laminaria japonica (Phaeophyceae). Fish. Sci., 67, 415-419 https://doi.org/10.1046/j.1444-2906.2001.00277.x
  18. Park, E.J. 2001. The effect of light and temperature on the growth and maturation of gametophytes in three Laminaria species from Korea. Master Thesis, Pukyong National University, Korea, 1-38
  19. Provasoli, L. 1968. Media and prospects for the cultivation of marine algae. In Cultures and Collections of Algae. Watanabe, A. and A, Hattori, eds., Jap. Soc. Plant Physiol., 63-75
  20. Seki, H. and A. Suzuki. 1998. Biosorption of heavy metal ions to brown algae, Macrocysis pyrifera, Kjellmaniella crassiforia, and Undaria pinnatifida. J. Colloid Interface Sci., 206, 297-301 https://doi.org/10.1006/jcis.1998.5731
  21. Seki, H. and A. Suzuki. 2002. Kinetic study of metal biosorption to a brown alga, Kjellmaniella crassifolia. J. Colloid Interface Sci., 246, 259-262 https://doi.org/10.1006/jcis.2001.8090
  22. Yamamoto, H. 1986. Monthly changes in the occurrence and growth of Kjellmaniella crassifolia Miyabe. Bull. Fac. Fish., Hokkaido Univ., 37, 165-170
  23. Yoon, H.S., J.Y Lee, S.M. Boo and D. Bhattacharya. 2001. Phylogeny of Alariaceae, Laminariaceae, and Lessoniaceae (Phaeophyceae) based on plastidencoded RuBisCo spacer and nuclear-encoded ITS sequence comparisons. Mol. Phylogenet. Evol., 21, 231-243 https://doi.org/10.1006/mpev.2001.1009

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