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http://dx.doi.org/10.5657/kfas.2007.40.6.387

Separation and Preservation of the Male and Female Gametophytes of Kjellmaniella crassifolia Miyabe (Phaeophyta)  

Kim, Jin-Hee (East Sea Regional Fisheries Research Institute, NFRDI)
Kim, Young-Dae (East Sea Regional Fisheries Research Institute, NFRDI)
Song, Hong-In (West Sea Regional Fisheries Research institute, NFRDI)
Gong, Yong-Gun (East Sea Regional Fisheries Research Institute, NFRDI)
Lee, Sang-Beum (Faculty of Marine Bioscience and Technology, Kangnung National University)
Jin, Hyung-Joo (Faculty of Marine Bioscience and Technology, Kangnung National University)
Publication Information
Korean Journal of Fisheries and Aquatic Sciences / v.40, no.6, 2007 , pp. 387-393 More about this Journal
Abstract
We examined the conditions for separating and preserving the male and female gametophytes of Kjellmaniella crassifolia. The highest percentage of zygote germination (85%) was on semi-solid medium composed of 1.0% transfer gel agar at $15\;^{\circ}C$ and $20\;{\mu}mol/m^2/s$ after a 4-week culture. Zygote germination in PESI liquid medium was 93.5% at $20\;^{\circ}C$ and $20\;{\mu}mol/m^2/s$. The maximum zygote growth was $252{\pm}19.7\;{\mu}m$ on 1.0% transfer gel agar at $15\;^{\circ}C$ and $40\;{\mu}mol/m^2/s$ after 5-week culture, and was $76.7{\pm}2.8\;{\mu}m$ in PESI liquid medium at $20\;^{\circ}C$ and $40\;{\mu}mol/m^2/s$. The respective numbers of separated male and female gametophytes from germinated zygotes were 157 and 93 on 1.0% transfer gel agar and 14 and 28 in PESI liquid medium. The maximum growth of separated male and female gametophytes was $575{\pm}28.3\;{\mu}m$ at $5\;^{\circ}C$ and $60\;{\mu}mol/m^2/s$ and $686{\pm}35.4\;{\mu}m$ at $20\;^{\circ}C$ and $20\;{\mu}mol/m^2/s$ in PESI liquid medium after 3 weeks, respectively. The highest percentage fertilized was $93.3{\pm}5.8%$ at $15\;^{\circ}C$ and $20\;{\mu}mol/m^2/s$ in PESI liquid medium. These results show that the best conditions for the separation and preservation of gametophytes (male and female) consisted of culturing on 1.0% transfer gel agar at $15\;^{\circ}C$ and $20\;{\mu}mol/m^2/s$.
Keywords
Gametophytes; Kjellmaniella crassifolia; Separation; Preservation;
Citations & Related Records
Times Cited By KSCI : 2  (Citation Analysis)
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