Bulletin of the Korean Chemical Society

  • 제28권1호
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  • Pages.77-80
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  • 2007
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  • 0253-2964(pISSN)
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  • 1229-5949(eISSN)
대한화학회 (Korean Chemical Society)
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Oxidative Modification of Neurofilament-L by the Cytochrome c and Hydrogen Peroxide System

  • 발행 : 2007.01.20
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초록

As neurofilament proteins are major cytoskeletal components of neuron, abnormality of neurofilament is proposed in brain with neurodegenerative disorders such as Parkinson's disease (PD). Since oxidative stress might play a critical role in altering normal brain proteins, we investigated the oxidative modification of neurofilament-L (NF-L) induced by the reaction of cytochrome c with H2O2. When NF-L was incubated with cytochrome c and H2O2, the protein aggregation was increased in cytochrome c and H2O2 concentrationsdependent manner. Radical scavengers, azide, formate and N-acetyl cysteine, prevented the aggregation of NFL induced by the cytochrome c/H2O2 system. The formations of carbonyl group and dityrosine were obtained in cytochrome c/H2O2-mediated NF-L aggregates. Iron specific chelator, desferoxamine, prevented the cytochrome c/H2O2 system-mediated NF-L aggregation. These results suggest that the cytochrome c/H2O2 system may be related to abnormal aggregation of NF-L which may be involved in the pathogenesis of PD and related disorders.

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피인용 문헌

  1. Oxidative Modification of Neurofilament-L Induced by Endogenous Neurotoxin, Salsolinol vol.32, pp.9, 2011, https://doi.org/10.5012/bkcs.2011.32.9.3421
  2. Protection by Histidine Dipeptides against Acrolein-induced Neurofilament-L Aggregation vol.29, pp.9, 2007, https://doi.org/10.5012/bkcs.2008.29.9.1732
  3. Generation of Hydroxyl Radicals in the Reaction of Ferritin with Hydrogen Peroxide vol.30, pp.7, 2009, https://doi.org/10.5012/bkcs.2009.30.7.1644