Parasites, Hosts and Diseases

The Korea Society for Parasitology and Tropical Medicine (대한기생충학·열대의학회)
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In vitro protoscolicidal effects of hypertonic glucose on protoscolices of hydatid cyst

  • Hosseini Seyed Vahid (Department of Surgery, Gastroenterohepatology Research Center (GEHRC)) ;
  • Ghanbarzadeh Kurosh (Department of Surgery, Gastroenterohepatology Research Center (GEHRC)) ;
  • Barzin Zahra (Department of Parasitology and Mycology, School of Medicine, Shiraz University of Medical Sciences) ;
  • Sadjjadi Seyed Mahmoud (Department of Parasitology and Mycology, School of Medicine, Shiraz University of Medical Sciences) ;
  • Tanideh Nader (Laboratory Animal Research Center/Department of Pharmacology, Shiraz University of Medical Sciences) ;
  • Mehrabani Davood (GEHRC/Razi Vaccine and Serum Research Institute, School of Medicine, Shiraz University of Medical Sciences)
  • Published : 2006.09.01
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Abstract

To evaluate the protoscolicidal effects of various concentrations of hypertonic glucose, live protoscolices of sheep were exposed to 10%, 15%, 25% and 50% glucose solutions. Cetrimide (0.5%), silver nitrate (0.5%) and hypertonic saline (20%) were used as positive controls, while physiological saline was used as a negative control. After 1, 2 and 5 min, the protoscolicidal effects were determined by 1 % eosin. A 25% glucose solution had no significant protoscolicidal effect. However, a 50% glucose solution revealed higher protoscolicidal effect than 0.5% silver nitrate but weaker effect than 0.5% cetrimide; the effect was comparable with that of 20% hypertonic saline. The results showed that hypertonic glucose solution is highly effective in killing protoscolices of Echinococcus granulosus in vitro.

Keywords

References

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