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In vitro protoscolicidal effects of hypertonic glucose on protoscolices of hydatid cyst  

Hosseini Seyed Vahid (Department of Surgery, Gastroenterohepatology Research Center (GEHRC))
Ghanbarzadeh Kurosh (Department of Surgery, Gastroenterohepatology Research Center (GEHRC))
Barzin Zahra (Department of Parasitology and Mycology, School of Medicine, Shiraz University of Medical Sciences)
Sadjjadi Seyed Mahmoud (Department of Parasitology and Mycology, School of Medicine, Shiraz University of Medical Sciences)
Tanideh Nader (Laboratory Animal Research Center/Department of Pharmacology, Shiraz University of Medical Sciences)
Mehrabani Davood (GEHRC/Razi Vaccine and Serum Research Institute, School of Medicine, Shiraz University of Medical Sciences)
Publication Information
Parasites, Hosts and Diseases / v.44, no.3, 2006 , pp. 239-242 More about this Journal
Abstract
To evaluate the protoscolicidal effects of various concentrations of hypertonic glucose, live protoscolices of sheep were exposed to 10%, 15%, 25% and 50% glucose solutions. Cetrimide (0.5%), silver nitrate (0.5%) and hypertonic saline (20%) were used as positive controls, while physiological saline was used as a negative control. After 1, 2 and 5 min, the protoscolicidal effects were determined by 1 % eosin. A 25% glucose solution had no significant protoscolicidal effect. However, a 50% glucose solution revealed higher protoscolicidal effect than 0.5% silver nitrate but weaker effect than 0.5% cetrimide; the effect was comparable with that of 20% hypertonic saline. The results showed that hypertonic glucose solution is highly effective in killing protoscolices of Echinococcus granulosus in vitro.
Keywords
Echinococcus granulosus; hydatid cyst; protoscolices; protoscolicidal agents; hypertonic glucose solution;
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