DOI QR코드

DOI QR Code

리스테리아 식중독균 검출을 위한 광학식 바이오센서 개발

Development of a Fiber-Optic Biosensor for the Detection of Listeria monocytogenes

  • Kim G. (National Institute of Agricultural Engineering, RDA) ;
  • Choi K.H. (National Institute of Agricultural Engineering, RDA)
  • 발행 : 2006.04.01

초록

Frequent outbreaks of foodborne illness demand the need for rapid and sensitive methods for detection of these pathogens. Recent development of biosensor technology has a great potential to meet the need for rapid and sensitive pathogens detection from foods. An antibody-based fiber-optic biosensor and an automated reagents supply system to detect Listeria monocytogenes were developed. The biosensor for detection of Listeria monocytogenes in PBS and bacteria spiked food samples was evaluated. The automated reagents supply system eliminated cumbersome sample and detection antibody injection procedures that had been done manually. The biosensor could detect $10^4$ cfu/ml of Listeria monocytogenes in PBS. By using the fiber-optic biosensor, $2x10^8$ cfu/ml of Listeria monocytogenes in the food samples were detectable.

키워드

참고문헌

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  7. Tims, T.B. and D.V. Lim. 2003. Confirmation of viable E. coli O157:H7 by enrichment and PCR after rapid biosensor detection. Journal of Microbiological Methods 55: 141-147 https://doi.org/10.1016/S0167-7012(03)00133-7
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피인용 문헌

  1. Development of a Temperature Controller for Microwave-assisted Digestion System for Agricultural Samples vol.34, pp.5, 2009, https://doi.org/10.5307/JBE.2009.34.5.371
  2. Antimicrobial Effect of the Submerged Culture of Sparassis crispa in Soybean Curd Whey vol.20, pp.1, 2013, https://doi.org/10.11002/kjfp.2013.20.1.111