Applied Microscopy

Korean Society of Microscopy (한국현미경학회)
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Ultrastructures of Zinc-containing Mast Cells Found in the Rat Hindpaw after an Inflammatory Stimuli: Zinc Selenium Autometallography

랫드 발바닥 염증부위에서 관찰된 zinc함유 비만세포의 미세구조: 조직화학적 염색을 중심으로

  • Lee, Bo-Ye (Department of Anatomy, Gachon Medical School) ;
  • Kim, Yi-Suk (Department of Anatomy, Gachon Medical School) ;
  • Lee, Boeb-Y. (Department of Anatomy, College of Medicine, Konkuk University) ;
  • Lee, Hyun-Sook (Department of Anatomy, College of Medicine, Konkuk University) ;
  • Tak, Gye-Rae (Division of Biomedical Engineering, College of Medicine, Konkuk University) ;
  • Lee, Young-Il (Department of Anatomy, College of Medicine, Dankook University) ;
  • Lee, Jeoug-Yeol (Graduate School, College of Medicine, Hallym University) ;
  • Jo, Seung-Mook (Department of Anatomy, Gachon Medical School)
  • 이보예 (가천의과대학교 해부학과) ;
  • 김이석 (가천의과대학교 해부학과) ;
  • 이법이 (건국대학교 의과대학 해부학교실) ;
  • 이현숙 (건국대학교 의과대학 해부학교실) ;
  • 탁계래 (건국대학교 의료생명대학 의학공학부) ;
  • 이영일 (단국대학교 의과대학 해부학교실) ;
  • 이정열 (한림대학교 의과대학 대학원) ;
  • 조승묵 (가천의과대학교 해부학과)
  • Published : 2006.12.31
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Abstract

Mast cells (MCs) are granulated cells that play a pivotal role in allergic reaction and inflammation. The granules of mast cells are known to be rich in zinc (Zn). Male Sprague-Dawley rats were used. We injected $200{\mu}L$ of complete Freund's adjuvant (CFA) subcutaneously in the dorsal aspect of one hindpaw Finally, zinc selenium autometallography(AMG) was done by Danscher's method. The present study showed the ultrastructures of zinc-containing mast cells found in inflammatory area following an complete freund's adjuvant (CFA) inoculation into the rat hindpaw. At light microscopic level, mast cells were round or oval, at average $12{\mu}m$ in diameter, with many filopodia extending from the cell surface. Because the rather small and spherical nucleus was centrally placed; it was frequently obscured by the cytoplasmic granules, it sometimes could not be seen. Mast cells were distributed chiefly in the vicinity of small blood vessels. In most preparation many mast cells were ruptured and their granules escaped into the surrounding tissue. In electron micrographs, The secretory granules were at average $0.5{\mu}m$ in diameter and were limited by a membrane. The cell surface contained numerous microvilli and folds. Their interior was heterogenous in appearance. The nucleus was surrounded by large numbers of prominent vesicels and a well developed Golgi apparatus, but scant endoplasmic reticulum.

본 연구에서는 흰쥐 발바닥에 complete Freund's adjuvant(CFA)를 투여한 후 염증부위에서 관찰되는 비만세포의 분포양상 및 미세구조를 zinc selenium autometal-lography$(AMG^{ZnSe})$로 염색한 후 광학 및 전자현미경으로 관찰하였다. 광학현미경으로 관찰된 CFA투여에 의한 염증성 반응은 피하층에 국한되어 있었으며, zinc함유 비만세포는 주로 피하조직의 혈관 주변에 분포하고 있었다. AMG 염색에 의한 흑갈색을 띠는 과립이 세포질을 채우고 있었으며, 많은 비만세포의 경우 세포막이 파괴되어 과립이 주위조직으로 퍼져 관찰되었다. 전자현미경으로 관찰된 zinc함유 비만세포는 불규칙한 외형을 띠었으며, 표면에는 작은 돌기들이 관찰되었다. AMG염색에 따른 은입자(silver grains)가 세포질내 과립에 국한되었으며, 높은 전자 밀도의 동질성 기질로 구성되어 있었으며, 경계막으로 싸여 있었다. 세포질내 세포소기관 중에서 특히 골지 복합체가 잘 발달되어 있었으며, 형질내세망과 사립체는 비교적 적게 가지고 있었다. 이들 세포의 핵은 비교적 작고 둥글고, 세포의 중심부에 위치하였으며, 이질염색질로 이루어져 있었다.

Keywords

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