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Ultrastructures of Zinc-containing Mast Cells Found in the Rat Hindpaw after an Inflammatory Stimuli: Zinc Selenium Autometallography  

Lee, Bo-Ye (Department of Anatomy, Gachon Medical School)
Kim, Yi-Suk (Department of Anatomy, Gachon Medical School)
Lee, Boeb-Y. (Department of Anatomy, College of Medicine, Konkuk University)
Lee, Hyun-Sook (Department of Anatomy, College of Medicine, Konkuk University)
Tak, Gye-Rae (Division of Biomedical Engineering, College of Medicine, Konkuk University)
Lee, Young-Il (Department of Anatomy, College of Medicine, Dankook University)
Lee, Jeoug-Yeol (Graduate School, College of Medicine, Hallym University)
Jo, Seung-Mook (Department of Anatomy, Gachon Medical School)
Publication Information
Applied Microscopy / v.36, no.4, 2006 , pp. 271-277 More about this Journal
Abstract
Mast cells (MCs) are granulated cells that play a pivotal role in allergic reaction and inflammation. The granules of mast cells are known to be rich in zinc (Zn). Male Sprague-Dawley rats were used. We injected $200{\mu}L$ of complete Freund's adjuvant (CFA) subcutaneously in the dorsal aspect of one hindpaw Finally, zinc selenium autometallography(AMG) was done by Danscher's method. The present study showed the ultrastructures of zinc-containing mast cells found in inflammatory area following an complete freund's adjuvant (CFA) inoculation into the rat hindpaw. At light microscopic level, mast cells were round or oval, at average $12{\mu}m$ in diameter, with many filopodia extending from the cell surface. Because the rather small and spherical nucleus was centrally placed; it was frequently obscured by the cytoplasmic granules, it sometimes could not be seen. Mast cells were distributed chiefly in the vicinity of small blood vessels. In most preparation many mast cells were ruptured and their granules escaped into the surrounding tissue. In electron micrographs, The secretory granules were at average $0.5{\mu}m$ in diameter and were limited by a membrane. The cell surface contained numerous microvilli and folds. Their interior was heterogenous in appearance. The nucleus was surrounded by large numbers of prominent vesicels and a well developed Golgi apparatus, but scant endoplasmic reticulum.
Keywords
CFA; AMG; Zinc; Pain; Mast cells; Hindpaw; Rat;
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