Optimized Culture Condition and Enzyme Activity of the Mycelia of Clavicorona pyxidata

좀나무싸리버섯(Clavicorona pyxidata)의 균사체 최적 배양조건 및 세포효소활성 특성

  • Lee Tae-Hee (Dapartment of Life Science, College of Natural Science, Dongguk University) ;
  • Kim Jin-Man (Dapartment of Biology, Graduate School, Dongguk University) ;
  • Han Yeong-Hwan (Dapartment of Life Science, College of Natural Science, Dongguk University)
  • Published : 2006.06.01

Abstract

The culture conditions for the enhanced mycelial of Clavicorona pyxidata DGUM 29005 were investigated. The optimal temperature and pH for the mycelial growth were $24^{\circ}C$ and 5.0, respectively. It was shown that trehalose was the best supplement of carbon sources in Czapek-Dox medium as a minimal medium for enhanced mycelial growth. In general, inorganic nitrogen sources were better than organic ones for mycelial growth. Calcium nitrate was the best out of the inorganic nitrogen test. The appropriate phosphorous and vitamin were $Na_2HPO_4$ and p-aminobenzoic acid, respectively. After the mycelial of C. pyxidata DGUM 29005 was cultivated at $24^{\circ}C$ for 20 days in MEM broth(pH 5.0), the specific activities of both exomycelial and endomycelial enzymes were determined. Among the exomycelial enzyme assayed, the specific activity of laccase was much higher than those of other enzymes. However, little or no enzyme activities of ${\alpha}$-amylase, chitinase, lipase and pretense were found.

Clavicorona pyxidata DGUM 29005의 균사 생육을 위한 최적 배양조건 및 효소 활성을 조사하였다. 균사 생육을 위한 최적 온도 및 pH는 각각 $24^{\circ}C$ 및 5.0이었다. 사용된 복합배지 중 malt extract medium (MEM)에서 가장 좋은 균사 생육을 나타내었다. 최소배지로 Czapek-Dox 한천배지를 사용하고 탄소원으로 trehalose, mannitol, sucrose 및 maltose 첨가시 균사생육이 우수하였다. 전반적으로 무기질소원이 유기질소원 보다 균사 생육을 더 촉진하였으며, 무기질소원으로 calcium nitrate를 사용하였을 때 균사 생육이 가장 우수하였다. 인산원으로 $Na_2HPO_4$를 사용했을 때 균사생육이 촉진되었으며, 가장 우수한 비타민원은 p-aminobenzoic acid이었다. MEM 액체배지를 사용하여 $24^{\circ}C$에서 20일간 C. pyxidata DGUM 29005를 배양하여 균사외 분비효소 및 균사내 효소의 활성도를 측정한 결과, 균사외 분비 효소 중 laccase의 활성도가 다른 효소에 비해 높았으며, ${\alpha}$-amylase, chitinase, lipase 및 pretense의 활성도는 낮거나 없었다.

Keywords

References

  1. Bradford, M.M. 1976. A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72, 248-254 https://doi.org/10.1016/0003-2697(76)90527-3
  2. Choi, S.J., S.J. Kim, and Y.H. Han. 2004. Physiological characteristics and optimized culture conditions of the mycelia of Inonotus mikadoi. Kor. J. Microbiol. 40, 100-103
  3. Corner, E.J.H. 1970. A monograph of Clavaria and allied Genera. Beih. Nova Hedwigia 33, 1-299
  4. Dodd, J.L. 1972. The genus Clavicorona. Mycologia 64, 737-773 https://doi.org/10.2307/3757931
  5. Erkel, G. and T. Anke. 1992. Clavicoronic acid, a novel inhibitor of reverse transcriptases from Clavicorona pyxidata (Pers.: Fr.) Doty. J. Anibiotics 45, 29-37 https://doi.org/10.7164/antibiotics.45.29
  6. James, S.W. and D.J. McLaughlin. 1988. The influence of carbohydrate source and concentration and light on fruit body development in Clavicorona pyxidata. Mycologia 80, 89-98 https://doi.org/10.2307/3807498
  7. Koske, R.E. and C.R. Leathers. 1969. Sporophore production by species of Clavicorona in pure culture. Mycologia 61, 999-1002 https://doi.org/10.2307/3757644
  8. Lee, J.Y. 1988. Coloured Korean Mushrooms. p. 204-213. Academic Publishing Co., Ltd., Seoul
  9. Lee, T.H. 2004. Ph.D. thesis. University of Dongguk, Seoul, Korea
  10. Lee, T.H. and Y.H. Han. 2001. Enzyme activities of the fruit body of Ramaria botrytis DGUM 29001. Mycobiology 29, 171-175
  11. Lickey, E.B. 2002. Ph.D. thesis. University of Tennessee, Knoxville, Tennessee, USA
  12. Miller, O.K. 1977. Mushrooms of North America. E.P. Dutton. Academic Press, New York