Korean Journal of Microbiology (미생물학회지)

The Microbiological Society of Korea (한국미생물학회)
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Application of qDVC Method for Measuring Viable Cells in Lakes

호수 생태계에서 살아있는 세균을 측정하기 위한 qDVC 방법의 적용

  • Kim, Mi-Ree (Dept. of Environmental Science, Kangwon National University) ;
  • Seo, Eun-Young (Dept. of Environmental Science, Kangwon National University) ;
  • Choi, Seung-Ik (Institute of Environment Research, Kangwon National University) ;
  • Ahn, Tae-Seok (Dept. of Environmental Science, Kangwon National University)
  • Published : 2006.09.30
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Abstract

For measuring the viable cells in lakes, quantitative direct viable count (qDVC) method is applied. In the qDVC process, the final concentration of glycine is fixed as 2%. For confirming the effectiveness of qDVC for enumerating the viable cells, the viable bacterial numbers were measured by plate count, CTC reduction method and qDVC method at 5 different lakes. Among these 3 methods, the bacterial numbers by qDVC is $2.4{\sim}6.0$ times higher than those by the other 2 methods. And by the qDVC method, the viable cells were easily discriminated from dead or dormant cells.

호소수내의 '살아있는 세균'을 측정하기 위하여 quantitative direct viable count (qDVC) 방법을 적용하였다. qDVC방법에 적용되는 최적 glycine 농도는 2%였으며, '살아있는 세균'을 계수하는데 있어서 평판계수법, CTC법 보다는 qDVC 방법이 보다 효과적이라는 것을 확인하였다. qDVC방법으로 '살아있는 세균'을 측정한 결과 다른 두 방법보다 $2.4{\sim}6.0$배 높은 값이었다. 또한 qDVC방법은 '살아있는 세균'을 죽은 세포 또는 휴면세포와 쉽게 구별할 수 있었다.

Keywords

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