Journal of Microbiology and Biotechnology

  • 제16권5호
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  • Pages.786-790
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  • 2006
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  • 1017-7825(pISSN)
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  • 1738-8872(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
권호 표지

In Vivo Immunopotentiating Effects of Cellular Components from Lactococcus lactis ssp. lactis

  • Kim Ji-Yeon (Department of Food Standard Evaluation, Korea Food & Drug Administration) ;
  • Lee Seong-Kyu (Department of Applied Biological Chemistry, The University of Tokyo) ;
  • Jeong Do-Won (School of Agricultural Biotechnology, and Center of Agricultural Biomaterials, Seoul National University) ;
  • Hachimura Satoshi (Department of Applied Biological Chemistry, The University of Tokyo) ;
  • Kaminogawa Shuichi (Department of Food Science and Technology, College of Bioresource Science, Nihon University) ;
  • Lee Hyong-Joo (School of Agricultural Biotechnology, and Center of Agricultural Biomaterials, Seoul National University)
  • 발행 : 2006.05.01
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초록

Cellular components of Lactococcus lactis ssp. lactis (heat-killed whole cells, cytoplasm, and cell walls) were tested for their in vivo immunopotentiating activity. Peritoneal macrophages from mice orally administered with heat-killed whole cells exhibited significantly greater phagocytic activity than the groups administered with cell-wall fraction or cytoplasm fraction. The cytotoxicity of natural-killer cells was the highest in the group administered with whole cells, and the production of cytokines ($IFN-\gamma$, IL-2, and IL-12) in spleen cells was significantly higher, when cellular components were injected, and it tended to be higher in the cell-wall and cytoplasm groups than in the whole-cell group. Interestingly, the cytokine production of Peyer's patch cells was high, when cytoplasm fractions were administered. These results demonstrate that whole cells and cytoplasm and cell-wall fractions of L. lactis ssp. lactis have immunopotentiating activities, which are related to the stimulation of Peyer's patches.

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