Journal of Microbiology and Biotechnology

  • 제16권5호
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  • Pages.778-785
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  • 2006
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  • 1017-7825(pISSN)
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  • 1738-8872(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
권호 표지

Catechol 1,2-Dioxygenase from Rhodococcus rhodochrous N75 Capable of Metabolizing Alkyl-Substituted Catechols

  • Cha Chang-Jun (Department of Biotechnology, Chung-Ang University)
  • 발행 : 2006.05.01
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초록

Catechol 1,2-dioxygenase was purified from cells of R. rhodochrous N75 grown at the expense of benzoate and p-toluate as the sole sources of carbon. A single catechol 1,2-dioxygenase was found to be induced with either growth substrate. The enzyme has an estimated $M_r$ of 71,000 consisting of two identical subunits. Catechol 1,2-dioxygenase from R. rhodochrous N75 exhibits some unusual properties including: broad substrate specificity, extradiol cleavage activity with 4-methylcatechol and low $K_m$ values for halocatechols, suggesting that this enzyme is distinct from other known catechol and chlorocatechol 1,2-dioxygenases.

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