Korean Journal of Medicinal Crop Science (한국약용작물학회지)

The Korean Society of Medicinal Crop Science (한국약용작물학회)
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Effect of Ethylene Inhibitors on Plant Regeneration of Scrophularia buergeriana M.

에틸렌 억제 물질들이 현삼의 식물체재분화에 미치는 영향

  • Kim, Young-Kyung (Division of Plant Science & Resources, Chungnam National University) ;
  • Park, Dong-Sik (Kangwon National University, Research Institute of Agricultural Sciences) ;
  • Kim, Seong-Mu (Kangwon National University, School of Bioscience & Biotechnology) ;
  • Cho, Dong-Ha (Kangwon National University, School of Bioscience & Biotechnology) ;
  • Yu, Chang-Yeon (Bioherb Research Institute, Kangwon National University) ;
  • Park, Sang-Un (Division of Plant Science & Resources, Chungnam National University)
  • 김용경 (충남대학교 농업생명과학대학 식물자원학부) ;
  • 박동식 (강원대학교 농업과학연구소) ;
  • 김성무 (강원대학교 생명공학부) ;
  • 조동하 (강원대학교 생명공학부) ;
  • 유창연 (강원대학교 한방 바이오 연구소) ;
  • 박상언 (충남대학교 농업생명과학대학 식물자원학부)
  • Published : 2006.12.30
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Abstract

The study was carried out to establish an improved protocol for shoot organogenesis and plant regeneration from leaf explant cultures of Scrophularia buergeriana M. with the treatment of ethylene inhibitors [silver nitrate (AgNO$_3$), aminoethox-yvinylglycine (AVG), Cobalt chloride (CoCl$_2$)]. The regenerated shoots obtained from leaf explant cultures on MS medium containing 2 mg/l BAP, The additions of AgNO$_3$. AVG and CoC1$_2$ substantially improved the shoot regeneration frequency, at the optimal concentration of 7 mg/L, 7 mg/L, and 3 mg/L respectively, The regenerated shoots could be easily rooted with 0.1 mg/L IBA treatment. The noted plants were hardened and transferred to vermiculite with a 85% survival rate where they grew normally.

Keywords

References

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