Effect of Potassium Phosphate on Growth and Ginsenosides Biosynthesis from Ginseng Hairy Root

인삼모상근의 생장과 Ginsenoside 생합성에 미치는 KH$_2$PO$_4$의 영향

  • In, Jun-Gyo (Institute of Biotechnology, BioPia Co., Ltd.) ;
  • Park, Dong-Sik (Kangwon National University, Research Institute of Agricultural Sciences) ;
  • Lee, Bum-Soo (Institute of Biotechnology, BioPia Co., Ltd.) ;
  • Lee, Tae-Hoo (Department of Oriental Medicinal Materials and Processing, Kyung Hee University) ;
  • Kim, Se-Young (Department of Oriental Medicinal Materials and Processing, Kyung Hee University) ;
  • Rho, Yeong-Deok (Department of Oriental Medicinal Materials and Processing, Kyung Hee University) ;
  • Cho, Dong-Ha (Kangwon National University, School of Bioscience & Biotechnology) ;
  • Kim, Seong-Mu (Kangwon National University, School of Bioscience & Biotechnology) ;
  • Yang, Deok-Chun (Department of Oriental Medicinal Materials and Processing, Kyung Hee University)
  • 인준교 ((주)바이오피아 생명공학연구소) ;
  • 박동식 (강원대학교 농업과학연구소) ;
  • 이범수 ((주)바이오피아 생명공학연구소) ;
  • 이태후 (경희대학교 생명과학부) ;
  • 김세영 (경희대학교 생명과학부) ;
  • 노영덕 (경희대학교 생명과학부) ;
  • 조동하 (강원대학교 생명공학부) ;
  • 김성무 (강원대학교 생명공학부) ;
  • 양덕춘 (경희대학교 생명과학부)
  • Published : 2006.12.30

Abstract

To investigate effects on the growth and ginsenosides accumulation in ginseng hairy root, potassium phosphate was supplemented with 1.25, 2.5, and 5.0 mM concentration in 1/2 MS medium, respectively. Potassium phosphate supplement was increased the biomass and ginsenosides accumulation when it was dose at the concentration of 1.25 mM. And the growth rate of hairy root in the light condition was higher than in dark condition. The highest contents and productivity of ginsenosides were observed at the supplement of 1.25 mM potassium phosphate at the 7th day after the culture onset. Ginseng hairy root cultured in 20 L bioreactor supplemented with 1.25 mM KH$_2$PO$_4$ was increased the growth with 1,609 g (F${\cdot}$W) and ginsenosides content with 11.09 mg than those in control.

Keywords

References

  1. Ando T, Tanaka O, Shibata S (1971) Chemical studies on the oriental plant drugs. Comparative studies on the saponins and sapogenins of ginseng and related crude drugs. Syoyakugaku Zasshi 25:28-32
  2. Aoki M, Horiguchi I, Itho C, Yang G, Harada T, Yakuwa T (1992) Development of an aseptic plant tissue culture vessel system enabling ventilation air composition control and addition of nutrient solutions. J. Agri. Met. 48:29-37 https://doi.org/10.2480/agrmet.48.29
  3. Arya S, Liu JR, Eriksson T (1991) Plant regeneration from protoplasts of Panax ginseng (C. A. Meyer) through somatic embryogenesis. Plant Cell Rep. 10:277-281
  4. Butenko RG, Grushvilsky RV, Stepyan LI (1968) Organogenesis and embryogenesis in a tissue culture (Panax ginseng) and other Panax Species. Bot. Zh. 53:906-911
  5. Chang WC, Hsing TI (1978) Callus induction, growth and rhizogenesis from root of Panax ginseng on a defind medium. Nat. Sci. Coun. 6:770-772
  6. Chilton MD, Drummond MH, Merlo DJ, Nester EW (1978) Highly conserved DNA of Ti-plasmids overlaps T-DNA maintained in plant tumors. Nature 275:147-149 https://doi.org/10.1038/275147a0
  7. Choi KT, Park JC, Ahn IO (1990) Saponin production in tissue culture of ginseng. Kor. J. Ginseng Sci. 14:107-111
  8. Harn WJ, Vasil IK (1983) Somatic embryogenesis in sugar cane (Saccharam officinarum): Growth and plant regeneration from embryogenetic cell suspension culture. Ann. Bot. 51:719-726 https://doi.org/10.1093/oxfordjournals.aob.a086523
  9. In JG, Lee BS, Song WS, Yang DC (2004) Ginsenosides production through in vitro culture of adventitious roots induced from Panax ginseng 'Chungpoong', Kor. J. Plant Res. 17:7-13
  10. Jung G, Tepfer D (1987) Use of genetic transformation by the Ri T-DNA of Agrobacterium rhizogenes to stimulate bio-mass and tropane alkaloid production in Atopa belladonna and Calystegia Sepium roots grown in vitro. Plant Sci. 50:145-151 https://doi.org/10.1016/0168-9452(87)90151-8
  11. Jung HY, Kang SM, Kang YM, Kim YD, Yang JK, Chung YG, Choi MS (2003) Selection of optimal biotic elicitor on tropane alkaloid production of hairy root in Scopolia parviflora Nakai. Kor. J. Med. Crop Sci. 11(5):358-363
  12. Kuribayashi T, Okura M, Ohashi H (1971) Effects of light intensity and soil pH on growth (in Japanese). Syoyakugaku Zasshi. 25:110-116
  13. Lee SW, Cha SW, Hyun DY, Kim YC, Kang SW, Seong NS (2006) Effect of furrow directions on growth and yield in Panax ginseng C. A. Meyer. Kor. J. Med. Crop Sci. 14:221¬224
  14. Mizukami H, Tabira Y, Ellis BE (1993) Methyl jasmonateinduced rosmarinic acid biosynthesis in Lithospermum erythrorhizon cell suspension cultures. Plant Cell Rep. 12:706-709
  15. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue. Physiol. Plant. 15:473-497 https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
  16. Park HJ, Oh SY, Choi KH, Meang SJ, Yoon ES, Yang DC (2000) Effect of jasmonic and methyl jasmonate on the production of ginsenosides in the hairy roots of Korean ginseng (Panax ginseng C.A. Meyer). J. Ginseng Res. 24:74-78
  17. Staswick PE (1992) Jasmonate, genes, and fragrant signals. Plant Physiol. 99:804-807 https://doi.org/10.1104/pp.99.3.804
  18. Vasil V, Vasil IK (1981) Sormatic embryogenesis and plant regeneration from suspended cultures of Pearl millet. Ann. Sot. 47:669-678
  19. Yang DC, Kim YH, Yang DC, Min BH, Shin SL, Choi KT (1998) Selection of active grow hairy root lines in ginseng. Kor. Soc. of Plant Tiss. Cult. 25:525-530
  20. Yoo BS., Byun SY (2001) Characterization of batch culture and effect of varous elicitors on ginsenoside production in suspension cultures of Panax ginseng C.A. Mayer. Korean J. Biotechnol. Bioeng. 16:620-625
  21. Yamakawa T, Onomichi K, Kodama T, Minoda Y (1983) Production of anthocyanins by Vitis cells in suspension culture. Agric. BioI. Chem. 47:2185-2191 https://doi.org/10.1271/bbb1961.47.2185
  22. Yoshikawa T, Furuya T (1987) Saponin production by cultures of Panax ginseng transformed with Agrobacterium rhizogenes. Plant Cell Rep. 6:449-453
  23. 남기열 (1996) 최신고려인삼-성분 및 효능편. 한국인삼연초연구원, 천일인쇄사, 대전. p. 13-43
  24. 오승용, 박효진, 민병훈, 양계진, 양덕춘 (2000a) 인삼 모상근의 생장에 미치는 auxin과 casein hydrolysate의 영향. 고려인삼학회지 24:123-127
  25. 오승용, 박효진, 최경화, 양계진, 양덕춘 (2000b) Chitin과 chitosan 처리에 의한 인삼 모상근으로부터 ginsenoside 생산. 고려인삼학회지 24:68-73
  26. 최관삼, 인준교, 이영복 (1994) 포도와 미국자리공 세포현탁배양계 안토시아닌과 베타시아닌에 미치는 광의 효과. 식물조직배양학회지 21:47-53