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Effect of Ethylene Inhibitors on Plant Regeneration of Scrophularia buergeriana M.  

Kim, Young-Kyung (Division of Plant Science & Resources, Chungnam National University)
Park, Dong-Sik (Kangwon National University, Research Institute of Agricultural Sciences)
Kim, Seong-Mu (Kangwon National University, School of Bioscience & Biotechnology)
Cho, Dong-Ha (Kangwon National University, School of Bioscience & Biotechnology)
Yu, Chang-Yeon (Bioherb Research Institute, Kangwon National University)
Park, Sang-Un (Division of Plant Science & Resources, Chungnam National University)
Publication Information
Korean Journal of Medicinal Crop Science / v.14, no.6, 2006 , pp. 367-370 More about this Journal
Abstract
The study was carried out to establish an improved protocol for shoot organogenesis and plant regeneration from leaf explant cultures of Scrophularia buergeriana M. with the treatment of ethylene inhibitors [silver nitrate (AgNO$_3$), aminoethox-yvinylglycine (AVG), Cobalt chloride (CoCl$_2$)]. The regenerated shoots obtained from leaf explant cultures on MS medium containing 2 mg/l BAP, The additions of AgNO$_3$. AVG and CoC1$_2$ substantially improved the shoot regeneration frequency, at the optimal concentration of 7 mg/L, 7 mg/L, and 3 mg/L respectively, The regenerated shoots could be easily rooted with 0.1 mg/L IBA treatment. The noted plants were hardened and transferred to vermiculite with a 85% survival rate where they grew normally.
Keywords
ethylene inhibitors; organogenesis; plant regeneration; Scrophularia buergeriana M;
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