Korean Journal of Medicinal Crop Science (한국약용작물학회지)

The Korean Society of Medicinal Crop Science (한국약용작물학회)
권호 표지

Effects of White Light and UV Irradiation on Growth and Saponin Production from Ginseng Hairy Root

광 및 UV 조사가 인삼 모상근의 생장 및 사포닌 생합성에 미치는 영향

  • In, Jun-Gyo (Institute of Biotechnology, BioPia Co., Ltd.) ;
  • Park, Dong-Sik (Kangwon Nationall University, Research Institute of Agricultural Sciences) ;
  • Lee, Bum-Soo (Institute of Biotechnology, BioPia Co., Ltd.) ;
  • Kim, Se-Young (Department of Oriental Medicinal Materials and Processing, Kyung Hee University) ;
  • Rho, Yeong-Deok (Department of Oriental Medicinal Materials and Processing, Kyung Hee University) ;
  • Cho, Dong-Ha (Kangwon National Uuiversify, School of Bioscience & Biortechnology) ;
  • Kim, Seong-Mu (Kangwon National Uuiversify, School of Bioscience & Biortechnology) ;
  • Yang, Deok-Chun (Department of Oriental Medicinal Materials and Processing, Kyung Hee University)
  • 인준교 ((주)바아오피아 생명공학연구소) ;
  • 박동식 (강원대학교 농업과학연구소) ;
  • 이범수 ((주)바아오피아 생명공학연구소) ;
  • 김세영 (경희대학교 생명과학부) ;
  • 노영덕 (경희대학교 생명과학부) ;
  • 조동하 (강원대학교 생명공학부) ;
  • 김성무 (강원대학교 생명공학부) ;
  • 양덕춘 (경희대학교 생명과학부)
  • Published : 2006.12.30
Download PDF
⟨ Previous Next ⟩

Abstract

To investigate the effect of culture conditions on growth and ginsenosides accumulation, we cultured the ginseng hairy root under three different media, white light or ultra-violet irradiation. The MS/B5 medium containning MS basal salt and B5 vitamin was good for the growth and ginsenoside accumulation. The light during the culture period of ginseng hairy root was irradiated. The growth was abundant in the ginseng hairy root cultured in dark. But the ginsenosides accumulation was higher than in the ginseng hairy root cultured in the light irradiation. When the ginseng hairy root was cultured in 20 L bioreactor, the ginsenosides accumulation was observed at 34% higher than the hairy root cultured in dark. UV irradiated the ginseng hairy root during the culture period. The long time irradiation of UV was caused decreasing the growth of ginseng hairy root, but the accumulation of ginsenosidess was increased as to the irradiated time.

Keywords

References

  1. Ando T, Tanaka O, Shibata S (1971) Chemical studies on the oriental plant drugs. Comparative studies on the saponins and sapogenins of ginseng and related crude drugs. Syoyakugaku Zasshi 25:28-32
  2. Ahn JC, Jeong YJ, Lee KB, Kim OT, Hwang B (1999) The effect of various culture media on histological anantomy and saikosaponin content in hairy root culture of Buplurum falcatum. Kor. J. Med. Crop Sci. 7:251-256
  3. Beggs CJ, Stdzer-Jehle A, Wellman E (1985) Isoflavonoid formation as an indicator of UN stress in bean (Phaseolus vulgaris L.) Plant Physiol. 79:630-634 https://doi.org/10.1104/pp.79.3.630
  4. Choi KT, Park JC, Ahn IO (1990) Saponin production in tissue culture of ginseng. Kor. J. Ginseng Sci. 14:107-111
  5. Choi KT, Yang DC (1987) Characteristics of the growth of ginseng tumor callus. Kor. J. Ginseng Sci. 11:56-65
  6. Chilton MD, Drummond MH, Merlo DJ, Nester EW (1978) Highly conserved DNA of Ti-plasmids overlaps T-DNA maintained in plant tumors. Nature 275:147-149 https://doi.org/10.1038/275147a0
  7. Christen P, Roberts MF, Phillipson JD, Evans WC (1989) High yield production of tropane alkaloids by hairy root cultures of a Datura candida hybrid. Plant Cell Rep. 8:75-77 https://doi.org/10.1007/BF00716842
  8. Furuya T, Kojima H, Syono K, Ishill T (1970) Isolation of Panaxatriol from Panax ginseng callus. Chem. Pharm Bull. 18: 2371-2372 https://doi.org/10.1248/cpb.18.2371
  9. Furuya T, Kojima H, Syono K, Ishii T, Uotani K, Nishio M (1973) Isolation of saponins and sapogenins from callus tissue of Panax ginseng. Chem. Pharm Bull. 21:98-101 https://doi.org/10.1248/cpb.21.98
  10. Hagimori M, Matsumoto T, Obi Y (1982) Studies on the production of digitalis cardenolides by plant tissue culture. Plant Physiol. 69:653-656 https://doi.org/10.1104/pp.69.3.653
  11. Hamill JD, Parr AJ, Robins RJ, Rhodes MJC (1986) Secondary product formation by cultures of Beta vulqaris and Nicotiana rustica transformed with Agrobacterium rhizogenes. Plant Cell Rep. 5:111-114 https://doi.org/10.1007/BF00269247
  12. Hong SJ, Lee YW, Joo CN (1987) Biosynthesis of saponins in Panax ginseng. Kor. J. Ginseng Sci. 11:136-144
  13. Hwang SJ, Na MS, Lee JB, Hwang B (2000) Production of tannin from hairy root cultures of Rheum undulatum L. Kor. J. Med. Crop Sci. 8:250-258
  14. Joo CN, Kwak HS, Lee HB, Lee CH (1983) Biosynthesis of saponins in Panax ginseng C. A. Meyer. I. Probable sites of the biosynthesis of ginseng saponin from acetate. Kor. J. Ginseng Sci. 7:108-114
  15. Jung G, Tepfer D (1987) Use of genetic transformation by the Ri T-DNA of Agrobacterium rhizogenes to stimulate bio-mass and tropane alkaloid production in Atopa belladonna and Calystegia Sepium roots grown in vitro. Plant Sci. 50:145-151 https://doi.org/10.1016/0168-9452(87)90151-8
  16. Kamada H, Okamura N, Satake M, Harada H, Shimomura K (1986) Alkaloid production by hairy root cultures in Atropa belladonna. Plant Cell Rep. 5:239-242 https://doi.org/10.1007/BF00269811
  17. Kitagawa I, Taniyama T, Hayashi T, Yoshikawa M (1983) Malonyl-ginsenosidc-$Rb_{1}$, $Rb_{2}$, -Rc and Rd, four new malonylated dammarane-type triterpene oligosaccharides from Ginseng radix. Chem. Pharm Bull. 31:3353-3356 https://doi.org/10.1248/cpb.31.3353
  18. Laneri U, Franconi R, Altavista P (1990) Somatic mutagenesis of Gerbera jamesonii hyvr : irradiation and in vitro culture. Acta Horticul. 28:395-402
  19. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue. Physiol. Plant. 15:473-497 https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
  20. Ooms G, Twell D, Bossen ME, Harry J, Hoge C, Burrell MM (1986) Developmental regulation of RI $T_{L}$-DNA gene expression in roots, shoots and tubers of transformed potato (Solanum tuberosum cv. Desiree). Plant Mol. BioI. 6:321-330 https://doi.org/10.1007/BF00034939
  21. Ozeki Y (1996) Regulation of anthocyanin synthesis in carrot suspension cultured cells. J. Plant Res. 109:343-351 https://doi.org/10.1007/BF02344483
  22. Park HJ, Oh SY, Choi KH, Meang SJ, Yoon ES, Yang DC (2000) Effect of jasmonic and methyl jasmonate on the production of ginsenosides in the hairy roots of Korean ginseng (Panax ginseng CA. Meyer). J. Ginseng Res. 24:74-78
  23. Park KE, Chung HS, Song SD, Roh KS, Song JS (1997) The effects of UV-B radiation on photosynthetic electron transport of barley (Hordeum vulgare L.) leaves. J. Kor. Environ. Sci. Soc. 6:369-378
  24. Yang DC, Choi YE (2000) Production of transgenic plants via Agrobacterium rhizogenes mediated transformation of Panax ginseng. Plant Cell Rep. 19:491-496 https://doi.org/10.1007/s002990050761
  25. Yang DC, Kim YH, Yang DC, Min BH, Shin SL, Choi KT (1998) Selection of active grow hairy root lines in ginseng. Kor. J. Plant Tiss. Cult. 25(6):525-530
  26. Yang DC, Yun KY, Kim YH and Yang DC (2000) Physiological responses of hairy roots of ginseng (Panax ginseng C.A.Meyer) to iron status and pH change. Kor. J. Plant Tiss. Cult. 27:31-37
  27. Yoshikawa T, Furuya T (1987) Saponin production by cultures of Panax ginseng transformed with Agrobacterium rhizogenes. Plant Cell Rep. 6:449-453
  28. 남기열 (1996) 최신고려인삼-성분 및 효능편. 한국인삼연초연구원. 천일인쇄사. p. 13-44