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Effects of White Light and UV Irradiation on Growth and Saponin Production from Ginseng Hairy Root  

In, Jun-Gyo (Institute of Biotechnology, BioPia Co., Ltd.)
Park, Dong-Sik (Kangwon Nationall University, Research Institute of Agricultural Sciences)
Lee, Bum-Soo (Institute of Biotechnology, BioPia Co., Ltd.)
Kim, Se-Young (Department of Oriental Medicinal Materials and Processing, Kyung Hee University)
Rho, Yeong-Deok (Department of Oriental Medicinal Materials and Processing, Kyung Hee University)
Cho, Dong-Ha (Kangwon National Uuiversify, School of Bioscience & Biortechnology)
Kim, Seong-Mu (Kangwon National Uuiversify, School of Bioscience & Biortechnology)
Yang, Deok-Chun (Department of Oriental Medicinal Materials and Processing, Kyung Hee University)
Publication Information
Korean Journal of Medicinal Crop Science / v.14, no.6, 2006 , pp. 360-366 More about this Journal
Abstract
To investigate the effect of culture conditions on growth and ginsenosides accumulation, we cultured the ginseng hairy root under three different media, white light or ultra-violet irradiation. The MS/B5 medium containning MS basal salt and B5 vitamin was good for the growth and ginsenoside accumulation. The light during the culture period of ginseng hairy root was irradiated. The growth was abundant in the ginseng hairy root cultured in dark. But the ginsenosides accumulation was higher than in the ginseng hairy root cultured in the light irradiation. When the ginseng hairy root was cultured in 20 L bioreactor, the ginsenosides accumulation was observed at 34% higher than the hairy root cultured in dark. UV irradiated the ginseng hairy root during the culture period. The long time irradiation of UV was caused decreasing the growth of ginseng hairy root, but the accumulation of ginsenosidess was increased as to the irradiated time.
Keywords
Agrobacterium rhizogenes:ginsenosides; hairy root; light; UV;
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