Journal of Microbiology

The Microbiological Society of Korea (한국미생물학회)
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YlaC is an Extracytoplasmic Function (ECF) Sigma Factor Contributing to Hydrogen Peroxide Resistance in Bacillus subtilis

  • Ryu Han-Bong (School of Biological Sciences, and Institute of Microbiology, Seoul National University) ;
  • Shin In-Ji (School of Biological Sciences, and Institute of Microbiology, Seoul National University) ;
  • Yim Hyung-Soon (School of Biological Sciences, and Institute of Microbiology, Seoul National University) ;
  • Kang Sa-Ouk (School of Biological Sciences, and Institute of Microbiology, Seoul National University)
  • Published : 2006.04.01
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Abstract

In this study, we have attempted to characterize the functions of YlaC and YlaD encoded by ylaC and ylaD genes in Bacillus subtilis. The GUS reporter gene, driven by the yla operon promoter, was expressed primarily during the late exponential and early stationary phase, and its expression increased as the result of hydrogen peroxide treatment. Northern and Western blot analyses revealed that the level of ylaC transcripts and YlaC increased as the result of challenge with hydrogen peroxide. A YlaC-overexpressing strain evidenced hydrogen peroxide resistance and a three-fold higher peroxidase activity as compared with a deletion mutant. YlaC-overexpressing and YlaD-disrupted strains evidenced higher sporulation rates than were observed in the YlaC-disrupted and YlaD-overexpressing strains. Analyses of the results of native polyacrylamide gel electrophoresis of recombinant YlaC and YlaD indicated that interaction between YlaC and YlaD was regulated by the redox state of YlaD in vitro. Collectively, the results of this study appear to suggest that YlaC regulated by the YlaD redox state, contribute to oxidative stress resistance in B. subtilis.

Keywords

References

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