Analytical Science and Technology (분석과학)

The Korean Society of Analytical Sciences (한국분석과학회)
권호 표지
DOI QR코드

DOI QR Code

Analysis of malachite green and leuco-malachite green in sea food

수산식품 중 말라카이트그린 및 류코말라카이트그린의 분석

  • Choi, Dongmi (Center for Food Evaluation, Korea Food & Drug Administration) ;
  • Hong, Soongun (Center for Food Evaluation, Korea Food & Drug Administration) ;
  • Im, Moohyeog (Center for Food Evaluation, Korea Food & Drug Administration) ;
  • Jeong, Jiyoon (Center for Food Evaluation, Korea Food & Drug Administration) ;
  • Chang, Moonik (Center for Food Evaluation, Korea Food & Drug Administration) ;
  • Park, Kunsang (Center for Food Evaluation, Korea Food & Drug Administration) ;
  • Hong, Mooki (Center for Food Evaluation, Korea Food & Drug Administration) ;
  • Woo, Gunjo (Center for Food Evaluation, Korea Food & Drug Administration)
  • 최동미 (식품의약품안전청, 식품평가부) ;
  • 홍순근 (식품의약품안전청, 식품평가부) ;
  • 임무혁 (식품의약품안전청, 식품평가부) ;
  • 정지윤 (식품의약품안전청, 식품평가부) ;
  • 장문익 (식품의약품안전청, 식품평가부) ;
  • 박건상 (식품의약품안전청, 식품평가부) ;
  • 홍무기 (식품의약품안전청, 식품평가부) ;
  • 우건조 (식품의약품안전청, 식품평가부)
  • Received : 2005.01.06
  • Accepted : 2006.02.13
  • Published : 2006.04.27
Download PDF
⟨ Previous Next ⟩

Abstract

To determine malachite green and leuco-malachite green residues in sea food, a liquid chromatographic method has been optimized. The target compounds were extracted in the homogenized edible tissues with a mixture of McIlvaine buffer-acetonitrile and partitioned against dichloromethane. After concentrating the lower layer, the resulting residues were re-dissolved in methanol and analyzed by the HPLC with visible detector at 620 nm using acetonitrile-acetate buffer. For the analysis of leuco-malachite green with malachite green simultaneously, post-column packed with lead(IV) oxide was used for oxidizing leuco-malachite green to malachite green. The correlation coefficients($r^2$) was 0.9989 for malachite green, and 0.9995 for leuco-malachite green. The limit of detection was 0.005 mg/kg for the combined of malachite green and leuco-malachite green at signal/noise${\geq}3$. The recovery rate was within a reliable range of 84~98% (CV 3~16%). Leuco-malachite green were detected in carp and crusian carp.

고속액체크로마토그라피를 이용하여 어류 중 말라카이트그린 및 류코말라카이트그린의 최적 분석조건을 확립하고 국내유통 수산식품을 분석하였다. 균질화한 시료를 아세토니트릴로 추출하고 디클로로메탄에 분산시켜 농축 후 메탄올에 용해하여 분석하였다. 이동상으로는 아세토니트릴과 아세테이트 완충용액의 혼합용액을 사용하였으며, 검출파장은 620 nm이었다. 류코말라카이트그린을 말라카이트그린과 동시에 분석하기 위하여 산화납(IV) 컬럼을 분석컬럼 뒤에 연결하여 류코말라카이트그린을 말라카이트그린으로 산화하였다. 상관계수($r^2$)는 말라카이트그린이 0.9989, 류코말라카이트그린이 0.9995이었으며, 검출한계는 신호대 잡음비 3 이상에서 말라카이트그린과 류코말라카이트그린의 합으로서 0.005 mg/kg이었다. 회수율은 84~98%(CV는 3~16%)로 만족할 만한 수준이었다. 분석결과 잉어 및 붕어 서료에서 류코말라카이트그린이 검출되었다.

Keywords

References

  1. 보건복지부, 국민건강 . 영양조사, 2001
  2. 한국농촌경제연구원 식품수급표 2000, 2001
  3. '수산용 항생제 관리시스템 구축', 식품의약품안전청, 2003
  4. The Merck Veterinary Manual, 9th Ed., Merck & Co. Inc., USA, 2005
  5. 수산용약품사용안내, 국립수산과학원, 1996
  6. R. Durborow, D. Wise and J. Terhune, SRAC Publication No. 4700, 2003, (www.msstate.edu/dept/srac)
  7. The Merck Index, 13th Ed., Merck & Co. Inc., USA, 2001
  8. S. Srivastava, R and Sinha, D. Roy, Aquat. Toxicol., 66, 319-329(2004) https://doi.org/10.1016/j.aquatox.2003.09.008
  9. D. Dorge, M. Churchwell, T. Gehring Y. Pu and S. Plakas, Rapid Commun. Mass Spectrum., 12, 1625-1634 (1998) https://doi.org/10.1002/(SICI)1097-0231(19981115)12:21<1625::AID-RCM373>3.0.CO;2-I
  10. A. Bergwerft and P. Scherpenisse, J. Chromatogr. B, 788, 351-359(2003) https://doi.org/10.1016/S1570-0232(03)00042-4
  11. 동물용의약품등편람, 한국동물약품협회, 2001
  12. L. Rushing and E. Hansen, J. Chromatogr. B, 700, 223-231(1997) https://doi.org/10.1016/S0378-4347(97)00330-7
  13. A. Bergwerff, R. Kuiper and P. Scherpenisse, Aquaculture, 233, 55-63(2004) https://doi.org/10.1016/j.aquaculture.2003.11.013
  14. S. Turnipseed, J. Roybal, J. Hurlbut and A. Long, J. AOAG. Int., 78, 971-977(1995)
  15. C. Hajee and N. Haagsma, J. Chromatogr. B, 669, 219-227(1995) https://doi.org/10.1016/0378-4347(95)00112-V
  16. L. Rushing and H. Thompson Jr., J. Chromatogr. B, 688, 325-330(1997) https://doi.org/10.1016/S0378-4347(96)00298-8
  17. S. Turnipseed, J. Roybal, H. Rupp, J. Hurlbut and A. Long, J. Chromatogr. B, 670, 55-62(1995) https://doi.org/10.1016/0378-4347(95)00133-4
  18. L. Valle, C. Diaz, A. Zanocco and P. Richter, J. Chromatogr. B, 1067, 101-105(2005) https://doi.org/10.1016/j.chroma.2004.10.049
  19. P. Scherpenisse and A. Berbwerff, Anal. Chim. Acta, 529, 173-177(2005) https://doi.org/10.1016/j.aca.2004.08.009
  20. Codex guidelines for the establishmen of a regulatory programme for control of veterinary drug residues in foods, CODEX, CAC/GL 16,1993
  21. FAO/IAEA 동물용의약품잔류분석기술 훈련자료집, 국립수의과학검역원, 2004