Browse > Article
http://dx.doi.org/10.5806/AST.2006.19.2.142

Analysis of malachite green and leuco-malachite green in sea food  

Choi, Dongmi (Center for Food Evaluation, Korea Food & Drug Administration)
Hong, Soongun (Center for Food Evaluation, Korea Food & Drug Administration)
Im, Moohyeog (Center for Food Evaluation, Korea Food & Drug Administration)
Jeong, Jiyoon (Center for Food Evaluation, Korea Food & Drug Administration)
Chang, Moonik (Center for Food Evaluation, Korea Food & Drug Administration)
Park, Kunsang (Center for Food Evaluation, Korea Food & Drug Administration)
Hong, Mooki (Center for Food Evaluation, Korea Food & Drug Administration)
Woo, Gunjo (Center for Food Evaluation, Korea Food & Drug Administration)
Publication Information
Analytical Science and Technology / v.19, no.2, 2006 , pp. 142-148 More about this Journal
Abstract
To determine malachite green and leuco-malachite green residues in sea food, a liquid chromatographic method has been optimized. The target compounds were extracted in the homogenized edible tissues with a mixture of McIlvaine buffer-acetonitrile and partitioned against dichloromethane. After concentrating the lower layer, the resulting residues were re-dissolved in methanol and analyzed by the HPLC with visible detector at 620 nm using acetonitrile-acetate buffer. For the analysis of leuco-malachite green with malachite green simultaneously, post-column packed with lead(IV) oxide was used for oxidizing leuco-malachite green to malachite green. The correlation coefficients($r^2$) was 0.9989 for malachite green, and 0.9995 for leuco-malachite green. The limit of detection was 0.005 mg/kg for the combined of malachite green and leuco-malachite green at signal/noise${\geq}3$. The recovery rate was within a reliable range of 84~98% (CV 3~16%). Leuco-malachite green were detected in carp and crusian carp.
Keywords
malachite green; leuco-malachite green; sea food; HPLC;
Citations & Related Records
연도 인용수 순위
  • Reference
1 '수산용 항생제 관리시스템 구축', 식품의약품안전청, 2003
2 수산용약품사용안내, 국립수산과학원, 1996
3 The Merck Index, 13th Ed., Merck & Co. Inc., USA, 2001
4 L. Rushing and E. Hansen, J. Chromatogr. B, 700, 223-231(1997)   DOI
5 A. Bergwerff, R. Kuiper and P. Scherpenisse, Aquaculture, 233, 55-63(2004)   DOI   ScienceOn
6 S. Turnipseed, J. Roybal, H. Rupp, J. Hurlbut and A. Long, J. Chromatogr. B, 670, 55-62(1995)   DOI   ScienceOn
7 FAO/IAEA 동물용의약품잔류분석기술 훈련자료집, 국립수의과학검역원, 2004
8 The Merck Veterinary Manual, 9th Ed., Merck & Co. Inc., USA, 2005
9 S. Turnipseed, J. Roybal, J. Hurlbut and A. Long, J. AOAG. Int., 78, 971-977(1995)
10 R. Durborow, D. Wise and J. Terhune, SRAC Publication No. 4700, 2003, (www.msstate.edu/dept/srac)
11 Codex guidelines for the establishmen of a regulatory programme for control of veterinary drug residues in foods, CODEX, CAC/GL 16,1993
12 C. Hajee and N. Haagsma, J. Chromatogr. B, 669, 219-227(1995)   DOI   ScienceOn
13 S. Srivastava, R and Sinha, D. Roy, Aquat. Toxicol., 66, 319-329(2004)   DOI   ScienceOn
14 L. Valle, C. Diaz, A. Zanocco and P. Richter, J. Chromatogr. B, 1067, 101-105(2005)   DOI
15 보건복지부, 국민건강 . 영양조사, 2001
16 P. Scherpenisse and A. Berbwerff, Anal. Chim. Acta, 529, 173-177(2005)   DOI   ScienceOn
17 한국농촌경제연구원 식품수급표 2000, 2001
18 동물용의약품등편람, 한국동물약품협회, 2001
19 D. Dorge, M. Churchwell, T. Gehring Y. Pu and S. Plakas, Rapid Commun. Mass Spectrum., 12, 1625-1634 (1998)   DOI   ScienceOn
20 A. Bergwerft and P. Scherpenisse, J. Chromatogr. B, 788, 351-359(2003)   DOI   ScienceOn
21 L. Rushing and H. Thompson Jr., J. Chromatogr. B, 688, 325-330(1997)   DOI