Korean Journal of Ichthyology (한국어류학회지)

The Ichthyological Society of Korea (한국어류학회)
권호 표지

Cytogenetic Analysis of an Intergeneric Hybrids between Korean Bullhead (Pseudobagrus fulvidraco) and Ussurian Bullhead (Leiocassis ussuriensis)

동자개(Pseudobagrus fulvidraco)와 대농갱이(Leiocassis ussuriensis)간 유도된 잡종의 세포유전학적 분석

  • Park, Sang-Yong (Department of Marine Biotechnology, Soonchunhyang University) ;
  • Kim, Dong-Jun (Department of Marine Biotechnology, Soonchunhyang University) ;
  • Lee, Yoon-A (Department of Marine Biotechnology, Soonchunhyang University) ;
  • Noh, Choong-Hwan (Marine Resources Research Department, KORDI) ;
  • Kim, Dong-Soo (Department of Aquaculture, Pukyong National University) ;
  • Bang, In-Chul (Department of Marine Biotechnology, Soonchunhyang University)
  • 박상용 (순천향대학교 해양생명공학과) ;
  • 김동준 (순천향대학교 해양생명공학과) ;
  • 이윤아 (순천향대학교 해양생명공학과) ;
  • 노충환 (한국해양연구원 해양자원연구본부) ;
  • 김동수 (부경대학교 양식학과) ;
  • 방인철 (순천향대학교 해양생명공학과)
  • Received : 2006.01.12
  • Accepted : 2006.02.14
  • Published : 2006.03.31
Download PDF
⟨ Previous Next ⟩

Abstract

Bagrid catfish hybrid between Korean bullhead (Pseudobagrus fulvidraco) and Ussurian bullhead (Leiocassis ussuriensis) was produced by artificial fertilization. Modal chromosome numbers of Korean bullhead, Ussurian bullhead and their hybrid were the same as 2n=52. The karyotypes were 22 metacentric (M), 22 submetacentacentric (SM), and 8 acrocentric (A) chromosomes for Korean bullhead, 22M+14SM+16A for Ussurian bullhead and 22M+18SM+12A for their hybrid. The hybrid bullhead karyotype consisted of each haploid set of parental species chromosomes. Erythrocytic sizes and DNA contents of hybrids were intermediate between two parental species.

동자개(Pseudobagrus fulvidraco), 대농갱이(Leiocassis ussuriensis) 및 이들간 잡종의 염색체수는 모두 2n=52로 동일하였으나, 핵형은 서로 상이하여 동자개가 22 metacentrics (M)+22 submetacentric (SM)+8 acrocentric (A) chromosome으로, 대농갱이는 22M+14SM+16A, 그리고 잡종은 22M+18SM+12A로 구성되어 있었다. 잡종은 동자개, 대농갱이 각각의 반수체로 구성되어 있었다. 적혈구 세포 및 핵의 크기, 표면적 및 체적을 측정한 결과 유도된 잡종은 양친의 중간값을 나타내었다. 동자개, 대농갱이 및 잡종의 DNA 함량을 측정한 결과, 동자개는 2.06 pg/cell, 대농갱이는 2.09 pg/cell, 동자개와 대농갱이 잡종은 2.08 pg/cell로 나타나, 잡종의 DNA 함량은 양친의 중간값으로 나타났다.

Keywords

Acknowledgement

Supported by : 순천향대학교

References

  1. Blanc, J.M. and B. Chevassus. 1979. Interspecific hybridization of salmonid fish. I. Hatching and survival up to the 15th day after hatching in $F_{1}$ generation hybrids. Aquaculture 18 : 21-34 https://doi.org/10.1016/0044-8486(79)90097-8
  2. Blanc, J.M. and H. Poisson. 1983. Parental sources of variation in hatching and early survival rates of Salmo trutta ♀$\times$Salvelinus fontinalis♂ hybrid. Aquaculture 32 : 115-122 https://doi.org/10.1016/0044-8486(83)90274-0
  3. Chevassus, B. 1979. Hybridization in salmonids : results and perspectives. Aquaculture 17 : 113-128 https://doi.org/10.1016/0044-8486(79)90047-4
  4. Chevassus, B., R. Guyomard, D. Chorrout and E. Quillet, 1983. Production of viable hybrids in salmonids by triplodization. Genet. Sel. Evol. 15 : 519-532 https://doi.org/10.1186/1297-9686-15-4-519
  5. Chevassus, B. 1984. Hybridization in fish. Aquaculture 33 : 245-128 https://doi.org/10.1016/0044-8486(83)90405-2
  6. Cheng, Q. and B. Zheng. 1987. Systematic Synopsis of Chinese Fishes. Science Press, Beijing pp. 1458 (in Chinese)
  7. Galbreath, P.F., K.J. Adams, P.A. Wheeler and G.H. Thorgaard. 1997. Clonal atlantic salmon$\times$brown trout hybrids produced by gynogenesis. J. Fish Biol. 50 : 1025- 1033
  8. Hickling, C.F. 1960. Malacca tilapia hybrids. J. Genet. 57 : 75-85
  9. Ihssen, P.E., L.R. McKay, I. McMillan and P.B. Phillips. 1990. Ploidy manipulation and gynogenesis in fishes: Cytogenetic and fisheries applications. Trans. Am. Fish. Soc. 119 : 698-717 https://doi.org/10.1577/1548-8659(1990)119<0698:PMAGIF>2.3.CO;2
  10. Kim, D.S., E.-H. Park and J.S. Kim. 1982. Karyotypes of nine species of the Korean catfishes (Teleostomi: Suriformes). Korea J. Genet. 4 : 57-68
  11. Kim, D.S., I.-G. Jeon and J.K. Lee. 1989. Karyotypes, DNA values and nuclear sizes of several scups (Teleostomi: Perciformes). Korean J. Ichthyol. 1 : 35-41 (in Korean)
  12. Kim, D.S., Y.K. Nam and I.-S. Park. 1995. Survival and karyological ananlysis of reciprocal diploid and triplid hybrids between mud loach (Misgurus mizolepis) and cyprinid loach (Misgurius anguillicaudatus). Aquaculture 135 : 257-265 https://doi.org/10.1016/0044-8486(95)01031-9
  13. Lee, C.-L. and I.-S. Kim. 1990. A taxonomic revision of the family bagridae (Pisces, Siluriformes) from Korea. Korean J. Ichthyol. 2 : 117-137
  14. Lemoine, H.L., Jr. and L.T. Smith. 1980. Polyploidy induced in brook trout by cold shock. Trans. Am. Fish. Soc. 109 : 626-631 https://doi.org/10.1577/1548-8659(1980)109<626:PIIBTB>2.0.CO;2
  15. Levan, A., K. Fredga and A.A. Sangerg. 1964. Nomenclature for centromeric positon on chromosoems. Ilereditas 52 : 201-220
  16. Mires, D. 1977. Theoretical and practical aspects of the production of all male tilapia hybrids. Bamidgeh 29 : 94-101
  17. Scheerer, P.D. and G.H. Thorgaard. 1983. Increased survival in salmonid hybrids by induced triploidy. Can. J. Fish. Aquat. Sci. 40 : 2040-2044 https://doi.org/10.1139/f83-235
  18. Schwartz, F.J. 1972. World literature to fish hybrids with an analysis by family, species and hybrid. In: Gulf Coast Res. Lab. Ocean Springs, MS, Publ. Gulf Coast Res. Lab. Mus. No. 3, 328 pp
  19. Sezaki, K. and H. Kobayashi. 1978. Comparison of erythrocytic size between diploid and tetraploid in spinous loach, Cobitis biwae, Bull. Jap. Soc. Sci. Fish. 44 : 851-854 https://doi.org/10.2331/suisan.44.851
  20. Smith, S.H. 1970. Trends in fishery managements of the Great Lakes. In: A Contury of Fisheries in North America (ed Benson, N. G.). Am. Fish. Soc., Washington, DC, Spec. Publ. 7 : 107-114
  21. Smith, S.H. 1972. The future of salmonid communities in the Lauretian Great Lakes, J. Fish. Res. Bd. Can. 29 : 951-957 https://doi.org/10.1139/f72-138
  22. Szarski, H. 1976. Cell size and nuclear DNA content in vertebrates. Inter. Rev. Cytol. 44 : 93-112 https://doi.org/10.1016/S0074-7696(08)61648-4
  23. 김익수.최 윤.이충렬.이용주.김병직.김지현. 2005. 한국어류대도감. 교학사, 서울, p. 615
  24. 박인석. 1993. 염색체 조작에 의한 어류의 생물공학. 제주대 해양연구소 연구보고, 17 : 93-114
  25. 박인석. 1995. 잡종화 기법을 이용한 어류의 유전육종. 군산대 해양개발연구소논문, 7 : 47-68
  26. 박인석.김병기.김종만.최경철.김동수. 1996. 무지개송어 (Oncorhynchus mykiss)와 은연어 (O. kisutch)간의 잡종 및 잡종 3배체 생산, 한국양식학회지 9 : 133-140
  27. 방인철.남윤권.노충환.박준택.한경호. 2001. 한국산 꺽지과 어류 3종의 세포유전학적 연구. 한국수산학회, 34 : 17-20
  28. 정문기. 1977. 한국어도보. 일지사, 서울. pp. 222-230
  29. 전상린. 1984. 한국산 동자개과 및 메기과 어류의 검색과 분포에 관하여, 상명여자대학논문집, 14 : 83-115
  30. 최기철.전상린.김익수.손영목. 1990. 원색 한국 담수어도감. 향문사, 서울, p. 276