Korean Journal of Ichthyology (한국어류학회지)

The Ichthyological Society of Korea (한국어류학회)
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Molecular Phylogeny and Distribution of Far Eastern Oryzias latipes Based on Mitochondrial Cytochrome b Gene Sequence

미토콘드리아 cytochrome b 유전자 염기서열 분석에 의한 극동지역 송사리의 계통과 지리적 분포의 상관관계

  • Eah, Jae-Yong (National Genome Information Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Yoo, Jeong-Ha (Department of Chemical & Bioengineering, Kyungwon University) ;
  • Kang, Tae-Wook (National Genome Information Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Kim, Moo-Sang (Department of Molecular Cellular and Developmental Biology, Yale University) ;
  • Kim, Chang-Bae (National Genome Information Center, Korea Research Institute of Bioscience and Biotechnology)
  • 어재영 (한국생명공학연구원 유전체정보센터) ;
  • 유정하 (경원대학교 화학생명공학과) ;
  • 강태욱 (한국생명공학연구원 유전체정보센터) ;
  • 김무상 ;
  • 김창배 (한국생명공학연구원 유전체정보센터)
  • Received : 2005.12.27
  • Accepted : 2006.03.06
  • Published : 2006.03.31
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Abstract

To examine the relationship of geological distribution and phylogenetic tree of O. latipes in the Far East, we analyzed cytochrome b (cyt b) gene in the mitochondrial genome. In this study we employed the entire sequence of cyt b of 53 samples collected from nine Korean locations and 117 cyt b data retrieved from the GenBank. From 170 Oryzias latipes cyt b sequence data, 142 different haplotypes were identified and phylogenetic relationship was reconstructed based on the dataset. According to the phylogeny, haplotypes were divided into three major haplogroups A, B and C, and their relationships were well correlated to their distributional patterns. Haplogroup A which is widely distribute in the southern part of Korea is separated in the geographical distribution from the haplogroup B which is found from China to the western part of Korea. Haplogroup C is only found in Japan.

본 연구에서는 극동지역 Oryzias latipes의 지리적 분포와 미토콘드리아 cytochrome b (cyt b)의 유전적 다양성과의 관계를 밝히기 위해 전국 9개의 지역에서 53개체를 채집하여 얻은 cyt b 염기서열과 GenBank에 등록되어있던 117개의 데이터를 종합하여 이를 142개의 haplotype으로 새로이 변환하였고, 이의 계통수를 작성 분석하였다. 분석 결과 극동지역 송사리는 3개의 haplogroup (A, B, C)으로 나뉘어지며, haplogroup A는 남한의 남한아지역을 중심으로 남한 전역에 분포하며, haplogroup B는 중국와 남한의 서한아지역에 걸쳐 분포하며, haplogroup C는 일본에만 분포하였다. Haplogroup A는 haplogroup B와 한반도내에서 지리적으로 가깝게 분포하고 있으나 계통적으로는 상당한 거리를 가지고 뚜렷하게 구별되었다. haplotype의 분지양상은 선행된 연구결과와 거의 일치하였다.

Keywords

Acknowledgement

Supported by : 환경부, 한국생명공학연구원

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