Korean Chemical Engineering Research

  • 제44권6호
  • /
  • Pages.555-562
  • /
  • 2006
  • /
  • 0304-128X(pISSN)
  • /
  • 2233-9558(eISSN)
한국화학공학회 (The Korean Institute of Chemical Engineers)
권호 표지

HCI 프로그램을 이용한 HPLC의 최적화 조건

Optimum Condition of HPLC by HCI Program

  • Jin, Chun Hua (Department of Chemical Engineering, Inha University) ;
  • Lee, Ju Weon (Department of Chemical Engineering, Inha University) ;
  • Row, Kyung Ho (Department of Chemical Engineering, Inha University)
  • 투고 : 2006.06.30
  • 심사 : 2006.08.30
  • 발행 : 2006.12.31
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

최근 액체크로마토그래피는 의학상이나 천연물질 분리에 많이 사용되고 있다. 특히 혼합물에서 유용성분을 분취하기 위해서는 우선 분석용 크로마토그래피의 조업조건이 결정되어야 한다. 때문에 정확한 수학적 모델링과 시뮬레이션은 크로마토그래피의 발전과 디자인 과정에서 필요한 부분이다. 인하대학교 고 순도분리연구실에서는 크로마토그래피의 최적화를 목적으로 HCI 프로그램을 개발하였다. 이 과정에서 최소한의 실험으로 보다 정확하고 빠른 최적 조업조건을 HCI 프로그램을 이용하여 구할 수 있다. 즉 이론단수를 이용하여 체류인자를 예측하는 세가지 모델식으로부터 용출곡선을 계산할 수 있으며 분리도와 분리시간을 기준으로 최적 이동상의 조건을 계산할 수 있다.

Recently, liquid chromatography (LC) has been used more frequently to separate drugs and natural substances. Especially, to selection of the solutes from the products, the operation condition of analytical chromatography should be necessarily determined. So accurate computer modeling and simulation of chromatographic performances has become a necessary part of the development and design of processes. High-Purity Separation Lab. Inha University developed the resulting HCI software for the purpose of the optimization of chromatographic performances. The HCI program was utilized to find the optimum operating condition more accurately and rapidly, reducing the number of many possible experiments. The elution profiles were calculated by the plate theory based on the three retention mechanism of capacity factor.

키워드

참고문헌

  1. Row, K. H., Principle and application of Liquid chromatography, Inha University(1999)
  2. Barth, T., Hoiland, S., Fotland, P., Askvik, K. M., Pedersen, B. S. and Borgund, A. E., 'Acidic Compounds in Biodegraded Petroleum,' Organic Geochemistry., 35(11-12), 1513-1525(2004) https://doi.org/10.1016/j.orggeochem.2004.05.012
  3. Bennett, B., Lager, A., Russell, C. A., Love, G. D. and Larter, S. R., 'Hydropyrolysis of Algae, Bacteria, Archaea and Lake Sediments; Insights into the Origin of Nitrogen Compounds in Petroleum,' Organic Geochemistry., 35(11-12), 1427-1439(2004) https://doi.org/10.1016/j.orggeochem.2004.07.006
  4. Kang, D. H. and Row, K. H., 'Fractionation of Soybean Phospholipids by Preparative High-performance Liquid Chromatography with Sorbents of Various Particle Size,' J. Chromatogr. A., 949, 217-223(2002) https://doi.org/10.1016/S0021-9673(01)01477-7
  5. Park, Y. K., Row, K. H. and Chung, S. T., 'Adsorption Characteristics and Separation of Taxol from Yew Tree by NP-HPLC,' Separation Purification Technology., 19(1-2), 27-37(2000) https://doi.org/10.1016/S1383-5866(99)00070-2
  6. Chung, K. K., Park, Y. K., Row, K. H. and Chung, S. T., 'Pretreatment step for Taxol Separation Process,' Chemical Industrial Science Technology, 16(11), 46-50(1998)
  7. Lee, Y. W., Row, K. H., So, M. S., Polunina, I. A. and Larin, A. V., 'Reversed-Phase HPLC Retention of Deoxyribonucleosides as a Function of Mobile Phase Composition,' J. Liquid Chromatogr., 18(15), 3077-3089(1995) https://doi.org/10.1080/10826079508010434
  8. Kim, J. D., Row, K. H., So, M. S., Polunina, I. A. and Larin, A. V., 'Chromatographic Behaviour of Deoxyribonucleosides with respect to Organic Modifier Content in the Mobile Phase,' J. Liquid Chromatogr., 18(15), 3091-3104(1995) https://doi.org/10.1080/10826079508010435
  9. Lee, Y. W., So, M. S., Lee, J. W., Chung, S. T. and Row, K. H. 'Retention Models of Capacity Factor with Different Compositions of Organic Modifier in RP-HPLC,' Korean J. Chem. Eng., 13(6), 578-584(1996) https://doi.org/10.1007/BF02706024
  10. Polunina, I. A., Choi, D. K., Row, K. H. and Larin, A. V., 'Adsorption Isotherm of Deoxyadenosine under the Conditions of a Reverse Phase Liquid Chromatography,' Colloid J., 58(6), 805-807(1996)
  11. Larin, A. V. and Row, K. H., 'Novel Relationship between the Capacity Factor and the Fraction of Organic Modifier in the Mobile Phase,' Russian J. of Anal. Chem., 52(10), 1064-1066(1997)
  12. Row, K. H., 'Comparison of Retention Models for the Dependence of Retention Factors on Mobile-Phase Composition in Reversed-Phase High- Performance Liquid Chromatography,' J. Chromatogr., 797(1-2), 23-31(1998) https://doi.org/10.1016/S0021-9673(98)00027-2
  13. Row, K. H. and Larin, A. V., 'A Chromatographic Theory based on the Concept of a Layer of Equilibrium Adsorption,' Korean J. Chem. Eng., 12(4), 442-447(1995) https://doi.org/10.1007/BF02705808
  14. Lee, Y. W., Row, K. H., So, M. S., Polunina, I. A. and Larin, A. V., 'Reversed-Phase HPLC Retention of Deoxyribonucleosides as a Function of Mobile Phase Composition,' J. Liquid Chromatogr., 18(15), 3077-3089(1995) https://doi.org/10.1080/10826079508010434
  15. Kim, J. D., Row, K. H., So, M. S., Polunina, I. A. and Larin, A. V. 'Chromatographic Behaviour of Deoxyribonucleosides with respect to Organic Modifier Content in the Mobile Phase,' J. Liquid Chromatogr., 18(15), 3091-3104(1995) https://doi.org/10.1080/10826079508010435
  16. Jung, Y. A., Jung, S. T. and, Row, K. H., 'Resolution of Taxol in Terms of Mobile Phase Compositions in RP-HPLC,' HWAHAK KONGHAK, 35(5), 712(1997)
  17. Sbyder, L. R. and Quarry, M. A., 'Computer Simulation in HPLC Method Development. Reducing the Error of Predicted Retention Times,' J. Liq. Chrom., 10(8-9), 1789(1987) https://doi.org/10.1080/01483918708066799
  18. Lee, Y. W., Row, K. H., So, M. S., Polunina, I. A. and Larin, A. V., 'Reversed-Phase HPLC Retention of Deoxyribonucleosides as a Function of Mobile Phase Composition,' J. Liquid Chromatogr., 18(15), 3077-3089(1995) https://doi.org/10.1080/10826079508010434
  19. Kim, J. D., Row, K. H., So, M. S., Polunina, I. A. and Larin, A. V., 'Chromatographic Behaviour of Deoxyribonucleosides with respect to Organic Mo difier Content in the Mobile Phase,' J. Liquid Chromatogr., 18(15), 3091-3104(1995) https://doi.org/10.1080/10826079508010435
  20. Lee, Y. W., So, M. S., Lee, J. W., Chung, S. T. and Row, K. H., 'Retention Models of Capacity Factor with Different Compositions of Organic Modifier in RP-HPLC,' Korean J. Chem. Eng., 13(6), 578-584(1996) https://doi.org/10.1007/BF02706024
  21. Polunina, I. A., Choi, D. K., Row, K. H. and Larin, A. V. 'Adsorption Isotherm of Deoxyadenosine under the Conditions of a Reverse Phase Liquid Chromatography,' Colloid J., 58(6), 805- 807(1996)
  22. Larin, A. V. and Row, K. H., 'New Relationship Between the Capacity Factor and the Fraction of Organic Modifier in the Mobile Phase,' Russian J. of Anal. Chem., 52(10), 966-968(1997)
  23. Row, K. H., 'Comparison of Retention Models for the Dependence of Retention Factors on Mobile-Phase Composition in Reversed-Phase High-Performance Liquid Chromatography,' J. Chromatogr., 797, 23-31(1998) https://doi.org/10.1016/S0021-9673(97)00969-2
  24. Row, K. H. and Larin, A. V., 'Experimental Study of Influence of Slight Deviation from Adsorption Isotherm Linearity on Elution Peak Profiles,' J. Chem. Eng. Japan, 28(6), 851-853(1995) https://doi.org/10.1252/jcej.28.851
  25. Row, K. H. and Larin, A. V., 'A Chromatographic Theory based on the Concept of a Layer of Equilibrium Adsorption,' Korean J. Chem. Eng., 12(4), 442-447(1995) https://doi.org/10.1007/BF02705808
  26. Row, K. H. and Larin, A. V., 'Computational Algorithm to Predict Peak Profiles in Preparative High-Performance Chromatography,' Korean J. Chem. Eng., 12(5), 512-515(1995) https://doi.org/10.1007/BF02705852
  27. Lee, J. W. and Row, K. H., 'Optimum of Solvent Selectivity and Gradient Mode for Deoxyribonucleoside in RP-HPLC,' HWAHAK KONGHAK., 35(5), 769-775(1997)
  28. Lee, J. W. and Row, K. H., 'Optimum condition for Resolution of Deoxyribonucleosides,' HWAHAK KONGHAK., 36(2), 343-346(1998)
  29. Lee, J. W. and Row, K. H., 'Separation of phospholipids in Step- Gradient Mode,' HWAHAK KONGHAK., 8(4), 694(1997)
  30. Lee, J. W. and Row, K. H., 'Determination of Retention Factors of Aromatic Compounds by Gradient-Elution Reverse-Phase High Performance Liquid Chromatography,' Korean J. Chem., 19(6), (2002)
  31. Lee, S. K. and Row, K. H., 'Optimum Separation Condition of Peptides in Reversed-phase Liquid Chromatography,' Journar of Chromatography B., 800(1-2), 115-120(2004) https://doi.org/10.1016/S1570-0232(03)00596-8
  32. Zheng, J. Z. and Row, K. H., 'Optimum Mobile Phase Condition for Resolving Isoflavones in RP-HPLC,' In preparation
  33. Jin, Y. Z. and Row, K. H., 'Separation Condition for Catechin Compounds by Retention Theory in RP-HPLC,' In preparation
  34. Jin, C. H., Lee, J. W. and Row, K. H., 'Optimum Condition of Mobile Phase Composition for Purine Compounds by HCI Program,' J. Korean Ind. Eng. Chem., 17(3), 317-320(2006)