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Optimum Condition of HPLC by HCI Program  

Jin, Chun Hua (Department of Chemical Engineering, Inha University)
Lee, Ju Weon (Department of Chemical Engineering, Inha University)
Row, Kyung Ho (Department of Chemical Engineering, Inha University)
Publication Information
Korean Chemical Engineering Research / v.44, no.6, 2006 , pp. 555-562 More about this Journal
Abstract
Recently, liquid chromatography (LC) has been used more frequently to separate drugs and natural substances. Especially, to selection of the solutes from the products, the operation condition of analytical chromatography should be necessarily determined. So accurate computer modeling and simulation of chromatographic performances has become a necessary part of the development and design of processes. High-Purity Separation Lab. Inha University developed the resulting HCI software for the purpose of the optimization of chromatographic performances. The HCI program was utilized to find the optimum operating condition more accurately and rapidly, reducing the number of many possible experiments. The elution profiles were calculated by the plate theory based on the three retention mechanism of capacity factor.
Keywords
Plate Theory; Retention Mechanism; Capacity Factor; HCI Program;
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