KSBB Journal

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Extraction of ${\beta}$-carotene from Ascidian Tunic [Halocynthia roretzi] using Supercritical Carbon Dioxide and Co-solvent

초임계 이산화탄소를 이용만 우렁쉥이 껍질로부터 ${\beta}$-carotene 추출

  • Kang, In-Sook (Institute of Seafood Science, Faculty of Food Science & Biotechnology, Pukyong National University) ;
  • Youn, Hyun-Seok (Institute of Seafood Science, Faculty of Food Science & Biotechnology, Pukyong National University) ;
  • Park, Ji-Yeon (Institute of Seafood Science, Faculty of Food Science & Biotechnology, Pukyong National University) ;
  • Chun, Byung-Soo (Institute of Seafood Science, Faculty of Food Science & Biotechnology, Pukyong National University)
  • 강인숙 (부경대학교 식품생명공학부 수산식품연구소) ;
  • 윤현석 (부경대학교 식품생명공학부 수산식품연구소) ;
  • 박지연 (부경대학교 식품생명공학부 수산식품연구소) ;
  • 전병수 (부경대학교 식품생명공학부 수산식품연구소)
  • Published : 2006.06.28
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Abstract

Dried raw Ascidians(Halocynthia roretzi) shells harvested from fish farms in southern coast area in Korea were used to extract ${\beta}$-carotene using supercritical carbon dioxide($SCO_2$) and with ethanol as a co-solvent at the range of temperatures and pressures, from 25 to $65^{\circ}C$ and 100 to 350 bar respectively. The size of the dried Ascidians shells was around $850{\mu}m$. The system used this study was a semi-batch flow type high pressure unit. The efficiency of ${\beta}$-carotene extraction using $SCO_2$ with and without co-solvent, ethanol, influenced to pressure and temperature changes. The highest solubility of ${\beta}$-carotene in $SCO_2$ was 1.35 mg/g for ${\beta}$-carotene at $35^{\circ}C$ and 350 bar. With addition of 2(v/v%) ethanol the recovery of ${\beta}$-carotene was 93%. As a result of using n-hexane and methanol for rinse, at $35^{\circ}C$ and 350 bar the amount of ${\beta}$-carotene by methanol rinse was 5 times higher than that of n-hexane rinse.

본 연구는 초임계 이산화탄소와 에탄올 보조용매를 사용하여 천연물질에 존재하는 기능성 물질을 효율적으로 분리하여 식품 및 의약산업에 이용할 수 있는 천연 기능성 소재를 제공할 수 있는 가능성을 보여 주었다. 초임계 이산화탄소와 보조용매 에탄올 3 mL/min를 사용하여 멍게로부터 ${\beta}$-carotene을 추출하기 위한 최적의 조건은 $35^{\circ}C$, 350 bar이었으며, 추출물질을 흡착하는 흡착칼럼으로부터 ${\beta}$-carotene을 회수하기 위한 rinse 용매는 methanol이 우수한 결과를 나타내었다. 따라서 초임계 이산화탄소를 사용한 멍게 껍질로부터 ${\beta}$-carotene 추출 공정이 상업화될 경우 기존의 재래식 유기용매 추출법에서 회수한 것에 비해 잔존 유기용매가 없고 환경 친화적 공정으로 식품, 의악품 산업 등의 고품질 원료 소재로 이용될 수 있을 것으로 사료된다.

Keywords

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