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Extraction of ${\beta}$-carotene from Ascidian Tunic [Halocynthia roretzi] using Supercritical Carbon Dioxide and Co-solvent  

Kang, In-Sook (Institute of Seafood Science, Faculty of Food Science & Biotechnology, Pukyong National University)
Youn, Hyun-Seok (Institute of Seafood Science, Faculty of Food Science & Biotechnology, Pukyong National University)
Park, Ji-Yeon (Institute of Seafood Science, Faculty of Food Science & Biotechnology, Pukyong National University)
Chun, Byung-Soo (Institute of Seafood Science, Faculty of Food Science & Biotechnology, Pukyong National University)
Publication Information
KSBB Journal / v.21, no.3, 2006 , pp. 194-198 More about this Journal
Abstract
Dried raw Ascidians(Halocynthia roretzi) shells harvested from fish farms in southern coast area in Korea were used to extract ${\beta}$-carotene using supercritical carbon dioxide($SCO_2$) and with ethanol as a co-solvent at the range of temperatures and pressures, from 25 to $65^{\circ}C$ and 100 to 350 bar respectively. The size of the dried Ascidians shells was around $850{\mu}m$. The system used this study was a semi-batch flow type high pressure unit. The efficiency of ${\beta}$-carotene extraction using $SCO_2$ with and without co-solvent, ethanol, influenced to pressure and temperature changes. The highest solubility of ${\beta}$-carotene in $SCO_2$ was 1.35 mg/g for ${\beta}$-carotene at $35^{\circ}C$ and 350 bar. With addition of 2(v/v%) ethanol the recovery of ${\beta}$-carotene was 93%. As a result of using n-hexane and methanol for rinse, at $35^{\circ}C$ and 350 bar the amount of ${\beta}$-carotene by methanol rinse was 5 times higher than that of n-hexane rinse.
Keywords
Ascidians(Halocynthia roretzi); ${\beta}$-carotene; co-solvent; supercritical carbon dioxide;
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