KSBB Journal

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Retention Mechanism of Caffeine and Tryptophan in Macroporous Poly[Methacrylic Acid-co-Ethylene Glycol Dimenthacrylate] Rods

매크로 다공성 Poly[Methacrylic Acid-co-Ethylene Glycol Dimenthacrylate] 막대에서 Caffeine과 Tryptophan의 체류 메카니즘

  • Jin, Longmei (Center for Advanced Bioseperation Technology and Department of Chemical Engineering, Inha University) ;
  • Yan, Hongyuan (Center for Advanced Bioseperation Technology and Department of Chemical Engineering, Inha University) ;
  • Zheng, Jinzhu (Center for Advanced Bioseperation Technology and Department of Chemical Engineering, Inha University) ;
  • Row, Kyung-Ho (Center for Advanced Bioseperation Technology and Department of Chemical Engineering, Inha University)
  • 김룡매 (인하대학교 화학공학과, 초정밀생물분리기술연구센터) ;
  • 염홍원 (인하대학교 화학공학과, 초정밀생물분리기술연구센터) ;
  • 정금주 (인하대학교 화학공학과, 초정밀생물분리기술연구센터) ;
  • 노경호 (인하대학교 화학공학과, 초정밀생물분리기술연구센터)
  • Published : 2006.10.30
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Abstract

Macroporous Poly(Methacrylic acid-co-Ethylene Glycol Dimethacrylate) Rods were in situ thermal initialized within a empty column($3.9{\times}150mm$) by free radical polymerization. The polymerization mixture was consisted of monomer, cross-linking monomer, porogenic solvent, initiator and control the ratio of these materials, column efficiency could be developed. Caffeine and tryptophan as separation substances and the retention mechanism of this kind of monolithic column was mainly hydrogen bond function.

MAA와 EGDMA를 단량체 및 가교제로 한 일체형 컬럼은 친수성의 구조표면을 하고 있어 쉽게 시료와 수소결합 등 강한 상호작용을 할 수 있다. 본 실험에서는 카페인과 트립토판을 분리물질로 선택하였으나 완전한 분리도를 얻을 수가 없었다. 이는 일체형 컬럼의 제조 시 여러 요인에 기인하다. 중합혼합반응물질의 양의 조성과 반응조건이 적절하지 못하는 등 원인으로 생각된다. 따라서 일체형 컬럼의 분리도를 향상시키기 위한 많은 실험이 요구된다.

Keywords

References

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