KSBB Journal

  • 제21권5호
  • /
  • Pages.389-393
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  • 2006
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  • 1225-7117(pISSN)
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  • 2288-8268(eISSN)
한국생물공학회 (The Korean Society for Biotechnology and Bioengineering)
권호 표지

한천분해효소를 생산하는 Agarivorans sp. JA-1의 배양조건 및 Fed-batch 배양

Production of ${\beta}$-agarase in Batch and Fed-batch Culture by Agarivorans sp. JA-1

  • 이송애 (신라대학교 공과대학 생명공학과) ;
  • 김진욱 (신라대학교 공과대학 생명공학과) ;
  • 정종근 (신라대학교 공과대학 생명공학과) ;
  • 김인혜 (신라대학교 의생명과학대학 제약공학과) ;
  • 이상현 (신라대학교 의생명과학대학 제약공학과) ;
  • 김상진 (한국 해양 연구원, 해양자원 연구본부) ;
  • 이재화 (신라대학교 의생명과학대학 제약공학과)
  • Lee, Song-Ae (Department of Bioscience and Biotechnology, College of Engineering, Silla University) ;
  • Kim, Jin-Uk (Department of Bioscience and Biotechnology, College of Engineering, Silla University) ;
  • Jung, Jong-Geun (Department of Bioscience and Biotechnology, College of Engineering, Silla University) ;
  • Kim, In-Hae (Department of Pharmaceutical Engineering, College of Medical Life Science, Silla University) ;
  • Lee, Sang-Hyeon (Department of Pharmaceutical Engineering, College of Medical Life Science, Silla University) ;
  • Kim, Sang-Jin (Department of Marine Resources Research, Korean Ocean Research and Development) ;
  • Lee, Jae-Hwa (Department of Pharmaceutical Engineering, College of Medical Life Science, Silla University)
  • 발행 : 2006.10.30
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초록

제주도 연안에서 분리 동정된 한천분해효소를 생산하는 Agarivorans sp. JA-1의 배양특성을 연구하였다. 균주가 기질로 이용하는 한천은 배지 내의 한천의 농도가 0.2%일 때 가장 효율적인 성장을 하였으며 4종의 탄소원에 대해서는 성장에 영향을 미치지 않는 것으로 나타났다. 배양시간에 따른 한천농도는 15시간 이후 모두 분해되었으며 점도의 변화도 이와 유사한 것으로 나타났다. 또한 fed-batch 배양에서 0.8 g 한천을 2회 첨가하였으며 한천을 첨가한 후 agarase생산이 증가되는 것으로 나타났다. 한천올리고당의 성분분석을 한 결과 중합도가 6인 당이 확인되었다.

Characteristics of ${\beta}$-agarase production of Agarivorans sp, JA-1 isolated from north-eastern sea of Jeju marine environment was studied. Optimal cell growth was definite that the medium containing agar is 0.2%. The decreasing pattern of viscosity and agar concentration was same and they reached almost zero after 15 hours. Fed-batch culture was studied to improve agarase productivity by Agarivorans sp. JA-1 in marine broth containing 2.0 g/L agar with intermittent addition of 0.8 g agar two times. The hydrolysis products were identified oligosaccharide of degrees of polymerization 6.

키워드

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