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Production of ${\beta}$-agarase in Batch and Fed-batch Culture by Agarivorans sp. JA-1  

Lee, Song-Ae (Department of Bioscience and Biotechnology, College of Engineering, Silla University)
Kim, Jin-Uk (Department of Bioscience and Biotechnology, College of Engineering, Silla University)
Jung, Jong-Geun (Department of Bioscience and Biotechnology, College of Engineering, Silla University)
Kim, In-Hae (Department of Pharmaceutical Engineering, College of Medical Life Science, Silla University)
Lee, Sang-Hyeon (Department of Pharmaceutical Engineering, College of Medical Life Science, Silla University)
Kim, Sang-Jin (Department of Marine Resources Research, Korean Ocean Research and Development)
Lee, Jae-Hwa (Department of Pharmaceutical Engineering, College of Medical Life Science, Silla University)
Publication Information
KSBB Journal / v.21, no.5, 2006 , pp. 389-393 More about this Journal
Abstract
Characteristics of ${\beta}$-agarase production of Agarivorans sp, JA-1 isolated from north-eastern sea of Jeju marine environment was studied. Optimal cell growth was definite that the medium containing agar is 0.2%. The decreasing pattern of viscosity and agar concentration was same and they reached almost zero after 15 hours. Fed-batch culture was studied to improve agarase productivity by Agarivorans sp. JA-1 in marine broth containing 2.0 g/L agar with intermittent addition of 0.8 g agar two times. The hydrolysis products were identified oligosaccharide of degrees of polymerization 6.
Keywords
Agarivorans sp. JA-1; agar; agarase; oligosaccharide; fed-batch culture;
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