The Korean Journal of Pesticide Science (농약과학회지)

The Korean Society of Pesticide Science (한국농약과학회)
권호 표지

Insecticidal Activity of Essential Oils against Larvae of Culex pipiens pallens

빨간집모기(Culex pipiens pallens) 유충에 대한 식물오일의 살충효과

  • Kang, Shin-Ho (Depatment of Plant Medicine, Chungbuk National University) ;
  • Kim, Min-Ki (Depatment of Plant Medicine, Chungbuk National University) ;
  • Seo, Dong-Kyu (Depatment of Plant Medicine, Chungbuk National University) ;
  • Kim, Gil-Hah (Depatment of Plant Medicine, Chungbuk National University)
  • 강신호 (충북대학교 농업생명환경대학 식물의학과) ;
  • 김민기 (충북대학교 농업생명환경대학 식물의학과) ;
  • 서동규 (충북대학교 농업생명환경대학 식물의학과) ;
  • 김길하 (충북대학교 농업생명환경대학 식물의학과)
  • Published : 2006.03.31
Download PDF
⟨ Previous Next ⟩

Abstract

Larvicidal activity of 34 plant essential oils were tested against the 4th instar larvae of Culex pipiens pallens. Among them, six oils (basil, fennel, caraway seed, lime, thyme red, and thyme white) showed more than 80% mortality at 100 mg $litre^{-1}$ concentration. GC and GC-MS analyses of the six essential oils and bioassay of their components revealed that anethole (a major component of fennel and basil oil) and (+)-limonene (a major component of caraway seed and lime oil) showed higher larvicidal activities than others. In addition to the above two components, ${\alpha}$-phellandrene, p-cymene, $\gamma$-terpinene, ${\beta}$-pinene, and thymol showed higher larvicidal activity than Bt product.

빨간집모기(Culex pipiens pallens) 유충에 대한 34종 식물오일의 살충력을 조사하였다. 80% 이상의 살충효과를 나타낸 오일은 6종(basil, fennel, caraway seed, lime, thyme red, thyme white)이었다. 각 오일의 구성성분을 GC와 GC-MS로 분석하고 각 성분의 살충력을 검정한 결과, basil 및 fennel 오일의 주요성분인 anethole과 caraway seed 오일 및 lime 오일의 주요성분인 (+)-limonene 이 높은 살충력을 나타내었다. 또한 ${\alpha}$-phellandrene, p-cymene, $\gamma$-terpinene, ${\beta}$-pinene, thymol 도 대조약제인 Bt제 보다 높은 살충효과를 나타내었다.

Keywords

References

  1. Ahero, B. L. (2003) Larvaecidal effects of aqueous extracts of Azadirachta indica (neem) on the larvae of Anopheles mosquito. African J. Biotech. 2: 325-327 https://doi.org/10.5897/AJB2003.000-1067
  2. Banerjee, S., S. Magdum, G. P. Kalena and A. Banerji (2001) Insect growth regulatory activity of naturally occurring quinones and their derivatives in Dysdercus lwenigii Fabr. (Hem., Pyrrhocoridae). J. Appl. Entomol. 125:25-30 https://doi.org/10.1046/j.1439-0418.2001.00489.x
  3. Bouwmeester, H. J., J. Gershenzon, M. C. J. M. Konings and R. Croteau (1998) Biosynthesis of the monoterpenes limonene and carvone in the fruit of caraway. Plant Physiol. 117: 901-912 https://doi.org/10.1104/pp.117.3.901
  4. Bowers, W. S., T. Ohta, J. S. Cleere and P. A. Marsella (1976) Discovery of insect anti-juvenile hormones in plants. Science. 193: 542-547 https://doi.org/10.1126/science.986685
  5. Chantraine, J.-M., D. Laurent, C. Ballivian, G. Saavedra, R. Ibanez and A. Vilaseca (1998) Insecticidal activity of essential oils on Aedes aegypti larvae. Phytother. Res. 12: 350-354 https://doi.org/10.1002/(SICI)1099-1573(199808)12:5<350::AID-PTR311>3.0.CO;2-7
  6. Chapagam, B. and Z. Wiesman (2005) Larvicidal effects of aqueous extracts of Balanites aegyptiaca (desert date) against the larvae of Culex pipiens pallens mosquitoes. African J. Biotech. 4: 1351-1354
  7. Charles, J-F. and C. Nielsen-LeRoux (2000) Mosquitocidal bacterial toxins: Diversity, mode of action and resistance phenomena. Mem. Inst. Oswaldo. Cruz, Rio de Janeiro. 95:201-20
  8. Chisholm, M. G., M. A. Wilson and G. M. Gaskey (2003) Characterization of aroma volatiles in key lime essential oils (Citrus aurantifolia Swingle) Aavour Fragr. J. 18:106-115 https://doi.org/10.1002/ffj.1172
  9. Davidson, N. A. (2001) Least-toxic alternatives for argentine ants, fleas, and white grubs of lawns. California Environmental Protection Agency. Department of Pesticide Regulation. Report No. PM-01-02
  10. Davis, M., B. Dinham and S. Williamson (1998) Pesticide residues in food. Pest Management Notes. No.8
  11. EI Hag, E. A., A. H. Nadi and A. A. Zaitoon (1999) Toxic and growth retarding effects of there plant extracts on Culex pipiens pallens (Diptera: Culicidae). Phytother. Res. 13:388-392 https://doi.org/10.1002/(SICI)1099-1573(199908/09)13:5<388::AID-PTR455>3.0.CO;2-U
  12. Finney, D. J. (1971) Probit analysis. Cambrige Univ. Press. London
  13. Ganguly, N. K., N. Medappa, V. K. Srivastava, P. Singh, L. Kant. B. Shah, V. Muthuswamy and N. C. Saxena (2003) Prospects of using herbal products in the control of mosquito vectors. ICMR bulletin. 33: 1-10
  14. Jacobson, M. (1975) Insecticides from plants: a review of the literature, 1954-1971. Agricultural Handbook 461, U.S Dept. Agri., Washington, D.C. p.138
  15. Jukie, M. and M. Milos (2005) Catalytic oxidation and antioxidant properties of thyme essential oils (Thymusvulgarae L.). Croatica Chemica ACTA 78:105-110
  16. Kabaru, J. M. and L. Gichia (2001) Insecticidal activity of extracts derived from different parts of the mangrove tree Rhizophora mucronata (Rhizophoraceae) Lam. against three arthropods. African J. Sci. and Technol. 2:44-49
  17. Keita, S. M., C. Vincent, J.-P. Sclnnit and A Belanger (2000) Essential oil composition of Ocimum basilicum L., O. gratissimum L. and O. suave L. in the Republic of Guinea. Flavour Fragr. J. 15:339-341 https://doi.org/10.1002/1099-1026(200009/10)15:5<339::AID-FFJ922>3.0.CO;2-H
  18. Kim, S. I., K. S. Chang, Y. C. Yang, B. S. Kim and Y. J. Ahn (2004) Repellency of aerosol and cream products containing fennel oil to mosquitoes under laboratory and field conditions. Pest Manag. Sci. 60:1125-1130 https://doi.org/10.1002/ps.921
  19. Mitchell, M. J., S. L. Smith, S. Johnson and E. D. Morgan (1997) Effects of the neem tree compounds azadirachtin, salannin, nimbin, and 6-desacetylnimbin on ecdysone 20-monooxygenase activity. Arch. Insect Bioch. and Physiol. 35:199-209 https://doi.org/10.1002/(SICI)1520-6327(1997)35:1/2<199::AID-ARCH18>3.0.CO;2-6
  20. Ohlendorf, B., M. L. Flint and M. Brush (1998) Mosquitoes. http://www.ipm.ucdavis.edu/pdf/pestnotes/pnmosquitoes.pdf
  21. Ozbek, H., S. Ugras, I. Bayram, I. Uygan, E. Erdogan, A Ozturk and Z. Huyut (2004) Hepatoprotective effect of Foeniculum vulgare essential oil: A carbon-tetrachloride induced liver fibrosis model in rats. Scand. J. Lab. Anim. Sci. 31:9-17
  22. Palevitch, D. and L. E. Cracker (1994) Volatile oils as potential insecticides. The herb, Spice and medicinal plant digest. 12:1-8
  23. Petersen, L. R. and A. A. Marfin (2002) West nile virus: a primer for the clinician. Annals of Internal Medicine 137: 173-179 https://doi.org/10.7326/0003-4819-137-3-200208060-00009
  24. Pina-Vaz. C., A G. Rodrigues. E. Pinto. S. Costa-de-Oliveira, C. Tavares, L. Salgueiro, C. Cavaleiro and M. J. Goncalves (2004) Antifungal activity of Thymus oils and their major compounds. JEADV. 18:73-78 https://doi.org/10.1111/j.1468-3083.2004.00886.x
  25. Pyrethrins & Pyrethroids (1998) National Pesticide Telecommunications Network Available; http://npic.orst.edu/factsheets/pyrethrins.pdf
  26. Redwane, A., H. B. Lazrek, S. Bouallam, M. Markouk, H. Arnarouch and M. Jana (2002) Larvicial activity of extracts from Querus lusitania var infectoria galls (oliv). J. Ethnopharm. 79:261-263 https://doi.org/10.1016/S0378-8741(01)00390-7
  27. Russell, G. B. (1977) Plant chemicals affecting insect development. The New Zealand Entomologist. 6:229-234 https://doi.org/10.1080/00779962.1977.9722255
  28. Sagar, S. K. and S. S. Sehgal (1996) Effects of aqueous extract of deoiled neem (Azadirachta indica A. juss) seed kernel and karanja (Pongamia glabra vent) seed kernel against Culex quinquefasciatus. J. Commun. Dis. 28:260-269
  29. Sartoratto, A., A. L. M. Machado, C. Delarmelina, G. M. Figueira, M. C. T. Duarte and V. L. G. Rehder (2004) Composition and antimicrobial activity of essential oils from aromatic plants used in Brazil. Brazilian J. Microbiol. 35:275-280
  30. Spurr. E. B. and P. G. McGregor (2003) Potential invertebrate antifeedants for toxic baits used for vertebrate pest control. New Zealand department of conservation. Science for Conservation 232
  31. Steinemann, A. (2004) Human exposure, health hazards, and environmental regulations. Environmental hnpact Assessment Review. 24:695-710 https://doi.org/10.1016/j.eiar.2004.06.002
  32. Tao, L.-M., Y. J.-Z, Yang. P.-J. Zhuang and Z.-H. Tang (2006) Effect of a mixture of iprobenfos and malathion on the development of malathion resistance in the mosquito Culex pipiens pallens Coq. Pest Manag. Sci. 62:86-90 https://doi.org/10.1002/ps.1125
  33. Traboulsi, A. F., K. Taoubi, S. El-Haj, J. M. Bessiere and S. Rammal (2002) Insecticidal properties of essential plant oils against the mosquito Culex pipiens molestus (Diptera: Culicidae). Pest Manag. Sci. 58:491-495 https://doi.org/10.1002/ps.486