Korean Journal of Pharmacognosy (생약학회지)

The Korean Society of Pharmacognosy (한국생약학회)
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The Effect of Juglandis Semen Extract on Improvement of tBHT-induced $Na^+-pump$ Inactivity in Rabbit Cerebral Cortex

호도약침액이 가토 뇌조직의 $Na^+-pump$활성 장애 개선에 미치는 영향

  • Kim, Dong-Hoon (Kim Dong-hoon Oriental Medical Clinic) ;
  • Song, Jong-Wook (Graduate Program in Neuroscience, Inje University) ;
  • Lee, Sang-Kil (Department of Physical Therapy, College of Biomedical Science & Engineering, Inje University) ;
  • Kim, Joo-Heon (Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University) ;
  • Hong, Yong-Geun (Graduate Program in Neuroscience, College of Biomedical Science & Engineering, Inje University)
  • 김동훈 (김동훈 한의원) ;
  • 송종욱 (인제대학교 대학원 뇌과학협동과정, 인제대학교 의생명공학대학) ;
  • 이상길 (인제대학교 물리치료학과) ;
  • 김주헌 (경상대학교 수의과대학 수의학과 및 동물의학연구소) ;
  • 홍용근 (인제대학교 대학원 뇌과학협동과정)
  • Published : 2006.12.30
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Abstract

This study was undertaken to determine whether Juglandis Semen (JAS) extract exerts protective effect against oxidant-induced inhibition of $Na^+-pump$ activity in cerebral cortex. $Na^+-pump$ activity was estimated by measuring ouabain-sensitive oxygen consumption. The oxygen consumption significantly inhibited by 1mM t-butylhydroperoxide (tBHP), which was prevented by addition of 2% JAS extract. The oxygen consumption was increased by an increase in $Na^+$ concentration from 5 to 100 mM, $K^+$ concentration from 0.5 to 10 mM, and $Mg^{2+}$ concentration from 0.2 to 5 mM. These changes in ion concentrations did not affect the inhibitory effect of tBHP and protective action of JAS on oxygen consumption. tBHP (l mM) produced a significant increase in lipid peroxidation in cerebral cortex, which was prevented by 2% JAS extraction. These results suggest that JAS exerts protective effect against tBHP-induced inhibition of $Na^+-pump$ activity in the cerebral cortex, probably through action as antioxidant.

Keywords

References

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