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The Effect of Juglandis Semen Extract on Improvement of tBHT-induced $Na^+-pump$ Inactivity in Rabbit Cerebral Cortex  

Kim, Dong-Hoon (Kim Dong-hoon Oriental Medical Clinic)
Song, Jong-Wook (Graduate Program in Neuroscience, Inje University)
Lee, Sang-Kil (Department of Physical Therapy, College of Biomedical Science & Engineering, Inje University)
Kim, Joo-Heon (Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University)
Hong, Yong-Geun (Graduate Program in Neuroscience, College of Biomedical Science & Engineering, Inje University)
Publication Information
Korean Journal of Pharmacognosy / v.37, no.4, 2006 , pp. 272-277 More about this Journal
Abstract
This study was undertaken to determine whether Juglandis Semen (JAS) extract exerts protective effect against oxidant-induced inhibition of $Na^+-pump$ activity in cerebral cortex. $Na^+-pump$ activity was estimated by measuring ouabain-sensitive oxygen consumption. The oxygen consumption significantly inhibited by 1mM t-butylhydroperoxide (tBHP), which was prevented by addition of 2% JAS extract. The oxygen consumption was increased by an increase in $Na^+$ concentration from 5 to 100 mM, $K^+$ concentration from 0.5 to 10 mM, and $Mg^{2+}$ concentration from 0.2 to 5 mM. These changes in ion concentrations did not affect the inhibitory effect of tBHP and protective action of JAS on oxygen consumption. tBHP (l mM) produced a significant increase in lipid peroxidation in cerebral cortex, which was prevented by 2% JAS extraction. These results suggest that JAS exerts protective effect against tBHP-induced inhibition of $Na^+-pump$ activity in the cerebral cortex, probably through action as antioxidant.
Keywords
Juglandis Semen (JAS); $Na^+-pump$; t-butylhydroperoxide (tBHP); cerebral cortex;
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