• Title/Summary/Keyword: Juglandis Semen (JAS)

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The Effect of Juglandis Semen Extract on Improvement of tBHT-induced $Na^+-pump$ Inactivity in Rabbit Cerebral Cortex (호도약침액이 가토 뇌조직의 $Na^+-pump$활성 장애 개선에 미치는 영향)

  • Kim, Dong-Hoon;Song, Jong-Wook;Lee, Sang-Kil;Kim, Joo-Heon;Hong, Yong-Geun
    • Korean Journal of Pharmacognosy
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    • v.37 no.4 s.147
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    • pp.272-277
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    • 2006
  • This study was undertaken to determine whether Juglandis Semen (JAS) extract exerts protective effect against oxidant-induced inhibition of $Na^+-pump$ activity in cerebral cortex. $Na^+-pump$ activity was estimated by measuring ouabain-sensitive oxygen consumption. The oxygen consumption significantly inhibited by 1mM t-butylhydroperoxide (tBHP), which was prevented by addition of 2% JAS extract. The oxygen consumption was increased by an increase in $Na^+$ concentration from 5 to 100 mM, $K^+$ concentration from 0.5 to 10 mM, and $Mg^{2+}$ concentration from 0.2 to 5 mM. These changes in ion concentrations did not affect the inhibitory effect of tBHP and protective action of JAS on oxygen consumption. tBHP (l mM) produced a significant increase in lipid peroxidation in cerebral cortex, which was prevented by 2% JAS extraction. These results suggest that JAS exerts protective effect against tBHP-induced inhibition of $Na^+-pump$ activity in the cerebral cortex, probably through action as antioxidant.

The Effects of t-butylhydroperoxide (tBHP) and Juglandis Semen on Brain Na+-Pump Activity (호도약침액(胡桃藥鍼液)이 가토(家兎) 뇌조직(腦組織)의 Na+-pump 활성(活性) 장애(障碍)에 미치는 영향(影響))

  • Kim, Dong-Hoon;Jang, Kyung-Jeon;Song, Choon-Ho;Ahn, Chang-Beohm
    • Journal of Pharmacopuncture
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    • v.2 no.1 s.2
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    • pp.13-25
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    • 1999
  • This study was undertaken to determine whether Juglandis Semen (JAS) extraction exerts protective effect against oxidant-induced inhibition of $Na^+$-pump activity in cerebral cortex. $Na^+$-pump activity was estimated by measuring ouabain-sensitive oxygen consumption. The oxygen consumption significantly inhibited by 1 mM t-butylhydroperoxide (tBHP), which was prevented by addition of 2% JAS extraction. The oxygen consumption was increased by an increase in $Na^+$ concentration from 5 to 100mM, $K^+$ concentration from 0.5 to 10 mM, and $Mg^{++}$ concentration from 0.2 to 5 mM. These changes in ion concentrations did not affect the inhibitory effct of tBHP and protective action of JAS on oxygen consumption. tBHP(1mM) produced a significant increase in lipid peroxidation in cerebral cortex, which was prevented by 2% JAS extraction. These result suggest that JAS exerts protective effect against tBHP-induced inhibition of $Na^+$-pump activity in the cerebral cortex, this effect may be due to by an antioxidant action.