Journal of Microbiology

The Microbiological Society of Korea (한국미생물학회)
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Effect of Mycelial Extract of Clavicorona pyxidata on the Production of Amyloid $\beta$-Peptide and the Inhibition of Endogenous $\beta$-Secretase Activity in vitro

  • Lee, Tae-Hee (Department of Life Science, College of Natural Science, Dongguk University) ;
  • Park, Young-Il (Department of Biology, Graduate School, Dongguk University) ;
  • Han, Yeong-Hwan (Department of Life Science, College of Natural Science, Dongguk University)
  • Published : 2006.12.31
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Abstract

Amyloid $\beta$-peptide (A$\beta$), which is a product of the proteolytic effect of $\beta$-secretase (BACE) on an amyloid precursor protein, is closely associated with Alzheimer's disease (AD) pathogenesis. There is sufficient evidence to suggest that a BACE inhibitor may reduce A$\beta$ levels, thus decreasing the risk of AD. In a previous study, an extract of Clavicorona pyxidata DGUM 29005 mycelia was found to inhibit the production of a soluble $\beta$-amyloid precursor protein (s$\beta$APP), A$\beta$, and BACE in neuronal cell lines. We sought to determine whether this mycelial extract exerts the same effect in human rhabdomyosarcoma A-204 and rat pheochromocytoma PC-12 cells. We found that the production of A$\beta$ decreased in a dose-dependent manner in the presence of the mycelial extract and that the concentration of A$\beta$ never exceeded $50{\mu}g/ml$. The presence of sAPP was detected in every culture medium to which the mycelial extract had been added and its concentration remained the same, regardless of the concentration of the extract used. Endogenous $\beta$-secretase activity in A-204 and PC-12 cellular homogenates also decreased in the presence of this extract. These cells, in culture, were not susceptible to the cytotoxic activity of the mycelial extract.

Keywords

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