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Effect of Mycelial Extract of Clavicorona pyxidata on the Production of Amyloid $\beta$-Peptide and the Inhibition of Endogenous $\beta$-Secretase Activity in vitro  

Lee, Tae-Hee (Department of Life Science, College of Natural Science, Dongguk University)
Park, Young-Il (Department of Biology, Graduate School, Dongguk University)
Han, Yeong-Hwan (Department of Life Science, College of Natural Science, Dongguk University)
Publication Information
Journal of Microbiology / v.44, no.6, 2006 , pp. 665-670 More about this Journal
Abstract
Amyloid $\beta$-peptide (A$\beta$), which is a product of the proteolytic effect of $\beta$-secretase (BACE) on an amyloid precursor protein, is closely associated with Alzheimer's disease (AD) pathogenesis. There is sufficient evidence to suggest that a BACE inhibitor may reduce A$\beta$ levels, thus decreasing the risk of AD. In a previous study, an extract of Clavicorona pyxidata DGUM 29005 mycelia was found to inhibit the production of a soluble $\beta$-amyloid precursor protein (s$\beta$APP), A$\beta$, and BACE in neuronal cell lines. We sought to determine whether this mycelial extract exerts the same effect in human rhabdomyosarcoma A-204 and rat pheochromocytoma PC-12 cells. We found that the production of A$\beta$ decreased in a dose-dependent manner in the presence of the mycelial extract and that the concentration of A$\beta$ never exceeded $50{\mu}g/ml$. The presence of sAPP was detected in every culture medium to which the mycelial extract had been added and its concentration remained the same, regardless of the concentration of the extract used. Endogenous $\beta$-secretase activity in A-204 and PC-12 cellular homogenates also decreased in the presence of this extract. These cells, in culture, were not susceptible to the cytotoxic activity of the mycelial extract.
Keywords
Alzheimer's disease; amyloid $\beta$-peptide; amyloid precursor protein; $\beta$-secretase; Clavicorona pyxidata;
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1 Hussain, I., D.J. Powell, D.R. Howlett, G.A. Chapman, L. Gilmour, P.R. Murdock, D.G. Tew, T.D. Meek, C. Chapman, K. Schneider, S.J. Ratcliffe, D. Tattersall, T.T. Testa, C. Southan, D.M. Ryan, D.L. Simmons, F.S. Walsh, C. Dingwall, and G. Christie. 2000. ASP1 (BACE2) cleaves the amyloid precursor protein at the beta-secretase site. Mol. Cell Neurosci. 16, 609-619   DOI   ScienceOn
2 Lee, T.H., Y.I. Park, and Y.H. Han. 2006b. Effect of mycelial extract of Clavicorona pyxidata on acetylcholinesterase and $\beta$-secretase activity in vitro. J. Microbiol. 44, 502-507   과학기술학회마을
3 Vassar, R. 1999. $\beta$-Secretase cleavage of Alzheimer's amyloid precursor protein by the transmembrane aspartic protease BACE. Science 286, 735-741   DOI   ScienceOn
4 Cai, H., Y. Wang, D. McCarthy, H. Wen, D.R. Borchelt, D.L. Price, and P.C. Wong. 2001. BACE1 is the major beta-secretase for generation of beta peptides by neurons. Nat. Neurosci. 4, 233-234   DOI   ScienceOn
5 Lee, T.H., J.M. Kim, and Y.H. Han. 2006a. Optimized cultured condition and enzyme activity of the mycelia of Clavicorona pyxidata. Kor. J. Microbiol. 42, 98-102   과학기술학회마을
6 Sinha, S., J.P. Anderson, R. Barbour, G.S. Basi, R. Caccavello, D. Davis, M. Doan, H.F. Dovey, N. Frigon, and J. Hong. 1999. Purification and cloning of amyloid precursor protein beta-secretase form human brain. Nature 402, 537-540   DOI   ScienceOn
7 Selkoe, D.J. 1991. The molecular pathology of Alzheimer's disease. Neuron 6, 487-498   DOI   ScienceOn
8 Capell, A., H. Steiner, M. Willem, H. Kaiser, C. Meyer, J. Walter, S. Lammich, G. Multhaup, and C. Haass. 2000. Maturation and pro-peptide cleavage of beta-secretase. J. Biol. Chem. 275, 30849-30854   DOI   ScienceOn
9 Bennett, B.D., S. Babu-Khan, R. Loeloff, J.C. Louis, E. Curran, M. Citron, and R. Vassar. 2000. Expression analysis of BACE2 in brain and peripheral tissues. J. Biol. Chem. 275, 20647-20651   DOI   ScienceOn
10 Haniu, M., P. Denis, Y. Young, E.A. Mendiaz, J. Fuller, J.O. Hui, B.D. Bennett, S. Kahn, S. Ross, T. Burgess, V. Katta, G. Rogers, R. Vassar, and M. Citron. 2000. Characterization of Alzheimer's beta-secretase protein BACE. A pepsin family member with unusual properties. J. Biol. Chem. 275, 21099-21106   DOI   ScienceOn
11 Koldamova, R.P., I.M. Lefterov, M. Staufenbiel, D. Wolfe, S. Huang, J.C. Glorioso, M. Walter, M.G. Roth, and J.S. Lazo. 2005. The liver X receptor ligand T0901217 decreases amyloid beta production in vitro and in a mouse model of Alzheimer's disease. J. Biol. Chem. 280, 4079-4088   DOI   ScienceOn
12 Acquati, F., M. Accarino, C. Nucci, P. Fumagalli, L. Jovine, S. Ottolenghi, and R. Taramelli. 2000. The gene encoding DRAP (BACE2), a glycosylated transmembrane protein of the aspartic protease family, maps to the down critical region. FEBS Lett. 468, 59-64   DOI   ScienceOn
13 Felsenstein, K.M., L.W. Hunihan, and S.B. Roberts. 1994. Altered cleavage and secretion of a recombinant $\beta$-APP bearing the Swedish familial Alzheimer's disease mutation. Nat. Genet. 6, 251   DOI   ScienceOn
14 Hass, C., C.A. Lemere, A. Capell, M. Citron, P. Seubert, D. Schenk, L. Lannfelt, and D.J. Selkoe. 1995. The Swedish mutation causes early-onset Alzheimer's disease by $\beta$-secretase cleavage within the secretase pathway. Nat. Med. 1, 1291-1296   DOI
15 Potempska, A., K. Mack, P. Mehta, K.S. Kim, and D.L. Miller. 1999. Quantification of sub-femtomole amounts of Alzheimer's amyloid $\beta$ peptides. J. Exp. Clin. 6, 14-21
16 Selkoe, D.J. 1989. Biochemistry of altered brain proteins in Alzheimer's disease. Annu. Rev. Neurosci. 12, 463-469   DOI   ScienceOn
17 Estus, S. 1997. Aggregated $\beta$-amyloid protein induces cortical neuronal apoptosis and concomitant 'apoptotic' pattern of gene induction. J. Neurosci. 17, 7736-7745   DOI
18 Huse, J.T., D.S. Pijak, G.J. Leslie, V.M. Lee, and R.W. Doms. 2000. Maturation and endosomal targeting of beta site amyloid precursor protein-cleaving enzyme. J. Biol. Chem. 275, 33729-33737   DOI   ScienceOn
19 Citron, M., T. Oltersdorf, C. Haass, L. McConlogue, A.Y. Hung, P. Seubert, C. Vigo-Palfrey, I. Lieberburg, and D.J. Selkoe. 1992. Mutation of the $\beta$-amyloid precursor protein in familial Alzheimer's disease increases $\beta$-protein production. Nature 360, 672-674   DOI   ScienceOn
20 DeMattos, R.B., K.R. Bales, D.J. Cummins, J.C. Dodart, S.M. Paul, and D.M. Holtzman. 2001. Peripheral anti-A beta antibody alters CNS and plasma A beta clearance and decrease brain A beta burden in a mouse model of Alzheimer's disease. Proc. Natl. Sci. USA. 98, 8850-8855
21 Lee, H.J., G.Y. Heo, K.B. Lee, I.K. Rhee, and K.S. Song. 1999. Inhibitory activities of basidiomycetes on prolyl endopeptidase, acetylcholinesterase, and coagulation. J. Kor. Soc. Agric. Chem. Biotechnol. 42, 336-343   과학기술학회마을
22 Yan, R., M.J. Bienkowski, M.E. Shuck, H. Miao, M.C. Troy, A.M. Pauley, J.R. Brashier, N.C. Stratman, W.R. Mathews, and A.E. Buhl. 1999. Membrane-anchored aspartyl protease with Alzheimer's disease beta-secretase activity. Nature 402, 533-537   DOI   ScienceOn
23 Walsh, D.M., M. Townsend, M.B. Podlisny, G.M. Shanker, J.V. Fadeeva, O.E. Agnaf, D.M. Hartley, and D.J. Selkoe. 2005. Certain inhibitors of synthetic amyloid $\beta$-peptide ($A{\beta}$) fibrillogensis block oligomerization of natural $A{\beta}$) and thereby rescue long-term potentiation. J. Neurosci. 25, 2455-2462   DOI   ScienceOn
24 Luo, Y., B. Bolon, S. Kahn, B.D. Bennett, S. Babu-Khan, P. Denis, W. Fan, H. Kha, J. Zhang, and Y. Gong. 2001. Mice deficient in BACE1, the Alzheimer's beta-secretase, have normal phenotype and abolished beta-amyloid generation. Nat. Neurosci. 4, 231-232   DOI   ScienceOn
25 Hiroshi, T. and T. Takeshi. 2001. Three novel alternatively spliced isoforms of the human beta-site amyloid precursor protein cleaving enzyme (BACE) and their effect on amyloid beta-peptide production. Neurosci. Lett. 307, 9-12   DOI   ScienceOn