한국임상수의학회지 (Journal of Veterinary Clinics)

  • 제23권4권
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  • Pages.411-415
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  • 2006
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  • 1598-298X(pISSN)
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  • 2384-0749(eISSN)
한국임상수의학회 (The Korean Society of Veterinary Clinics)
권호 표지

단순배양과 알긴산배양에서 개 연골세포의 증식에 있어 계대배양에 따른 아스코빅산의 영향

Effects of Ascorbic Acid on the Proliferation of Subcultured Canine Chondocytes in Monolayer and Alginate Beads Culture

  • Kim Gon-Hyung (Laboratory of Veterinary Surgery, College of Veterinary Medicine, Chungbuk National University) ;
  • Park Jin-Uk (Laboratory of Veterinary Surgery, College of Veterinary Medicine, Chungbuk National University) ;
  • Hossain Mohammad Alamgir (Laboratory of Veterinary Surgery, College of Veterinary Medicine, Chungbuk National University) ;
  • Cho Ki-Rae (Laboratory of Veterinary Surgery, College of Veterinary Medicine, Chungbuk National University) ;
  • Kim Joong-Hyun (Laboratory of Veterinary Surgery, College of Veterinary Medicine, Chungbuk National University) ;
  • Choi Seok-Hwa (Laboratory of Veterinary Surgery, College of Veterinary Medicine, Chungbuk National University)
  • 발행 : 2006.12.30
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초록

아스코빅산은 세포 증식촉진을 위해 배지에 첨가하여 사용되나, 증식 촉진 작용기전에 대해서는 구체적으로 밝혀져 있지 않으며 연골세포의 계대배양에 따른 효과에 대해서는 보고된바 없다. 제 1, 2그리고 4계대배양한 개 연골세포의 단순배양과 알긴산 배양을 아스코빅산 첨가 배지와 무첨가 배지에서 실시하였다. 세포증식은 2,3-Bis(2-methoxy-4-nitro-5- sulfophenyl )-2H-tetrazolium - 5-carboxanilide inner salt)(XTT)검사법을 이용하였다. 아스코빅산 첨가에 의해 세포증식 촉진효과가 모든 계대배양 세포에서 관찰되었으나, 제 1계대배양 연골세포에서 아스코빅산의 세포능식 촉진 효과가 제 2, 4계대배양 연골세포보다 유의차 있게 높게 나타났다 (p<0.05). 아스코빅산 무첨가 배지에서 계대배양에 따른 세포 증식에 차이가 관찰되지 않았다. 따라서 아스코빅산은 낮은 계대배양단계의 연골세포에 있어 더욱 효과적인 세포증식 촉진효과가 있는 것으로 판단된다.

Ascorbic acid has been used widely as a medium supplement to stimulate cell proliferation, but its effects on cell proliferation have not yet been elucidated, and no reports have analyzed effects on subcultured chondrocytes. Subcultured canine chondrocytes of passage one, two and four were cultured in monolayer and alginate beads with and without ascorbic acid. Cell proliferation was examined by 2,3-Bis(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide inner salt) (XTT) colormetric assay. Ascorbic acid stimulated cell proliferation significantly in both culture methods (p<0.05). The increased cell numbers by stimulation with ascorbic acid were significantly high in passage one cells compared to that of other passages. Differences in cell proliferative capacity by subculturing were not determined. These results suggest that ascorbic acid stimulated the proliferation of subcultured canine chondrocytes and enhanced it more in low-passage cells than in the other cells tested.

키워드

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