Research in Plant Disease (식물병연구)

The Korean Society of Plant Pathology (한국식물병리학회)
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Crown Gall of Chrosanthemum Caused by Agrobacterium rubi and A. tumefaciens

Agrobacterium rubi와 A. tumefaciens에 의한 국화 뿌리혹병

  • Lee, Young-Kee (Plant Pathology Division, Department of Agricultural Biology, National Institute of Agricultural Science and Technology, RDA) ;
  • Park, Kyung-Seok (Plant Pathology Division, Department of Agricultural Biology, National Institute of Agricultural Science and Technology, RDA) ;
  • Hwang, Hye-Kyung (Plant Pathology Division, Department of Agricultural Biology, National Institute of Agricultural Science and Technology, RDA) ;
  • Hwang, Tae-Ho (Plant Pathology Division, Department of Agricultural Biology, National Institute of Agricultural Science and Technology, RDA) ;
  • Kim, Jin-Young (Gyeonggi-do Agricultural Research and Extension Services) ;
  • Lee, Jae-Kook (Entomology Division, Department of Agricultural Biology, National Institute of Agricultural Science and Technology, RDA) ;
  • Cha, Jae-Soon (Department of Plant Medicine, College of Agriculture, Life and Environment Sciences, Chungbuk National University)
  • 이영기 (농업과학기술원 농업생물부 식물병리과) ;
  • 박경석 (농업과학기술원 농업생물부 식물병리과) ;
  • 황혜경 (농업과학기술원 농업생물부 식물병리과) ;
  • 황태호 (농업과학기술원 농업생물부 식물병리과) ;
  • 김진영 (경기도농업기술원) ;
  • 이재국 (농업과학기술원 농업생물부 농업해충과) ;
  • 차재순 (충북대학교 농업생명환경대학 식물의학과)
  • Published : 2006.12.01
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Abstract

Crown gall on lower stem and root of chrysanthemum(Dendranthema grandiflorum Kitamura) was observed at Hwasung and Gumi in 2001 and 2004, respectively. Tumors were semi-round with rough surface texture of dark brown color. Nine isolates inducing gall formation on lower stem of chrysanthemum among twenty isolates from the tumor tissues were characterized. Their colonies were convex, glistening, circular with an entire edge and whitish or tannish cream in color on potato dextrose agar supplemented with 0.5% $CaCO_3$. The virulent isolates were rod-shaped with peritrichous flagellae, gram negative, aerobic and growing on D1M agar. Among nine virulent isolates, one isolate was identified as Agrobacterium rubi and eight isolates were A. tumefaciens based on biochemical and physiological characteristics, fatty acid profiles and substrate utilization patterns. A. tumefaciens had strong pathogenicity and broad host range compared with A. rubi. This is the first report on crown gall of chrysanthemum in Korea. To our knowledge, crown gall of chrysanthemum caused by A. rubi is first report in this study worldwide.

2001년 경기도 화성시와 2004년 경상북도 구미시에서 국화의 줄기와 뿌리에 뿌리혹병이 발생하였다. 혹의 모양과 색은 상이하였으나 표면이 거칠고 갈변된 타원형이었다. 혹 조직으로부터 분리한 20개 균주 중 국화의 유묘에 접종하여 혹을 형성한 9개 균주에 대하여 분류학적 특성을 조사하였다. 0.5% $CaCO_3$가 첨가된 PDA배지에서 생장된 콜로니들은 둥글고 볼록하면서 광택이 나는 크림색이었다. 모든 균주는 다수의 편모를 가진 간상의 세균으로 그람음성이면서 호기적으로 생장하였으며 D1M agar 에서도 생장하였다. 병원성 세균들은 대조균주와 상이한 특성을 가지고 있었으나 주요 생리 생화학적특성, 탄소원 이용양상, 지방산조성 비교 결과에 근거하여 8개 균주는 Agrobacterium tumefaciens로 1개 균주는 A. rubi로 동정되었다. A. tumefaciens 계통들은 A. rubi 계통보다 병원성이 강하였으며 기주범위가 넓었다. 국내에서 A. tumefaciens와 A. rubi에 의한 국화의 뿌리혹병은 본 연구에서 처음 보고되는 것이며 자연상태에서 A. rubi에 의해 국화의 뿌리혹병이 발생된 사례는 전 세계적으로 처음이다.

Keywords

References

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