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http://dx.doi.org/10.5423/RPD.2006.12.3.197

Crown Gall of Chrosanthemum Caused by Agrobacterium rubi and A. tumefaciens  

Lee, Young-Kee (Plant Pathology Division, Department of Agricultural Biology, National Institute of Agricultural Science and Technology, RDA)
Park, Kyung-Seok (Plant Pathology Division, Department of Agricultural Biology, National Institute of Agricultural Science and Technology, RDA)
Hwang, Hye-Kyung (Plant Pathology Division, Department of Agricultural Biology, National Institute of Agricultural Science and Technology, RDA)
Hwang, Tae-Ho (Plant Pathology Division, Department of Agricultural Biology, National Institute of Agricultural Science and Technology, RDA)
Kim, Jin-Young (Gyeonggi-do Agricultural Research and Extension Services)
Lee, Jae-Kook (Entomology Division, Department of Agricultural Biology, National Institute of Agricultural Science and Technology, RDA)
Cha, Jae-Soon (Department of Plant Medicine, College of Agriculture, Life and Environment Sciences, Chungbuk National University)
Publication Information
Research in Plant Disease / v.12, no.3, 2006 , pp. 197-204 More about this Journal
Abstract
Crown gall on lower stem and root of chrysanthemum(Dendranthema grandiflorum Kitamura) was observed at Hwasung and Gumi in 2001 and 2004, respectively. Tumors were semi-round with rough surface texture of dark brown color. Nine isolates inducing gall formation on lower stem of chrysanthemum among twenty isolates from the tumor tissues were characterized. Their colonies were convex, glistening, circular with an entire edge and whitish or tannish cream in color on potato dextrose agar supplemented with 0.5% $CaCO_3$. The virulent isolates were rod-shaped with peritrichous flagellae, gram negative, aerobic and growing on D1M agar. Among nine virulent isolates, one isolate was identified as Agrobacterium rubi and eight isolates were A. tumefaciens based on biochemical and physiological characteristics, fatty acid profiles and substrate utilization patterns. A. tumefaciens had strong pathogenicity and broad host range compared with A. rubi. This is the first report on crown gall of chrysanthemum in Korea. To our knowledge, crown gall of chrysanthemum caused by A. rubi is first report in this study worldwide.
Keywords
Agrobacterium rubi; A. tumefaciens; Chrysanthemum; Crown gall; Dendranthema grandiflorum;
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1 Bouzar, H. and Jones, J. B. 1992. Distinction of biovar 2 strains of Agrobacterium from other chromosomal groups by differential acid production. Lett. Appl. Microbiol. 15: 83-85   DOI
2 Miller, L. T. 1982. Single derivatization method for routine analysis of bacterial whole-cell fatty acid methyl esters, including hydroxy acids. J. Clin. Microbiol. 16: 584-586
3 Sawada, H., Ieki, H., Oyaizu, H. and Matsumoto, S. 1993. Proposal for rejection of Agrobacterium tumefaciens and revised descriptions for the genus Agrobacterium and for Agrobacterium radiobacter and Agrobacterium rhizogenes. Int. J. Syst. Bacteriol. 43: 694-702   DOI   ScienceOn
4 Schaad, N. W., Jones, J. B. and Chun, W. 2001. Laboratory guide for identification of plant pathogenic bacteria, 3rd ed. APS Press, St. Paul, Minn. 373 pp
5 Young, J. M., Kuykendall, L. D., Martinez-Romero, E., Kerr, A. and Sawada, H. 2001. A revision of Rhizobium Frank 1889, with an emended description of the genus, and the inclusion of all species of Agrobacterium Conn 1942 and Allorhizobium undicola de Lajudie et al. 1998 as new combinations: Rhizobium radiobacter, R. rhizogenes, R. rubi, R. undicola and R. vitis. Int. J. Syst. Evol. Microbiol. 51: 89-103   DOI
6 Lee, Y. K., Lee, J. H., Kim, J. Y., Cho, W. D. and Cha, J. S. 2003. Occurrence of crown gall of chrysanthemum caused by Agrobacterium tumefaciens. Plant Pathol. J. 19: 332
7 Allen, O. N. and Holding, A. J. 1974. Genus II. Agrobacterium Conn 1942. In: Bergey's manual of determinative bacteriology, 8th ed. by Buchannan, R. E. and Gibbons, N. E., eds., pp. 264- 267. The Williams and Wilkings, Baltimore
8 Sawada, H. and Ieki, H. 1992. Phenotypic characteristics of genus Agrobacterium. Ann. Phytopath. Soc. Japan 58: 37-45   DOI
9 Smith, E. F. and Townsend, C. 1907. A plant tumor of bacterial origin. Science 25: 671-673   DOI
10 Ohta, K. and Nishiyama, K. 1984. Studies on the crown gall diseases of flower crops. I. Occurrence of the Disease and the characterization of the causal bacterium. Ann. Phytopath. Soc. Japan 50: 197-204   DOI
11 Miller, H. N. 1975. Leaf, stem, crown and root galls induced in chrysanthemum by Agrobacterium tumefaciens. Phytopathology 65: 805-811   DOI
12 Bouzar, H., Chilton, W. S., Nesme, X., Dessaux, Y., Vaudequin, V., Petit, A., Jones, J. B. and Hodge, N. C. 1995. A new Agrobacterium strain isolated from aerial tumors on Ficus benjamina L. Appl. Environ. Microbiol. 61: 65-73
13 New, P. B. and Milne, K. S. 1976. Etiology and control of crown gall on potted chrysanthemums. N. Z. J. Exp. Agric. 4: 109- 115
14 Kersters, K. and De Ley, J. 1984. Genus Agrobacterium. In: Bergey's manual of systematic bacteriology, Vol. 1. by Krieg, N. R. and Holt, J. G., eds., pp. 244-254. The Williams and Wilkins, Baltimore
15 농림부. 2006. 2005년 화훼재배현황. 143 pp
16 Skerman, V. B. D., McGowan, V. and Sneath, P. H. A. 1980. Approved lists of bacterial names. Int. J. Syst. Bacteriol. 30: 225-420   DOI
17 Anderson, A. R. and Moore, L. W. 1979. Host specificity in the genus Agrobacterium. Phytopathology 69: 153-158
18 Bouzar, H., Jones, J. B. and Hodge, N. C. 1993. Differential characterization of Agrobacterium species using carbonsource utilization patterns and fatty acid profiles. Phytopathology 83: 733-739   DOI
19 Bush, A. and Pueppke, S. G. 1991. Characterization of an unusual new strain of Agrobacterium tumefaciens from Chrysanthemum morifolium Ram. Appl. Environ. Microbiol. 57: 2468-2472
20 Bazzi, C., Gozzi, R. and Mazzucchi, V. 1989. Translocation of Agrobacterium tumefaciens biovar 1 in stems of Chrysanthemum fructescens L. Phytopathol. Mediterr. 28: 28-32
21 van Zyl, F. G. H., Strijdom, B. W. and Staphorst, J. L. 1986. Susceptibility of Agrobacterium tumefaciens strains to two agrocin-producing Agrobacterium strains. Appl. Environ. Microbiol. 52: 234-238
22 Bazzi, C. and Rosciglione, B. 1982. Agrobacterium tumefaciens biotype 3, causal agent of crown gall in Chrysanthemum in Italy. Phytopathol. Z. 103: 280-284   DOI
23 Perry, K. L. and Kado, C. I. 1982. Characteristics of Ti plasmids from broad-host-range and ecologically specific biotype 2 and 3 strains for Agrobacterium tumefaciens. J. Bacteriol. 151: 343-350