THE EXPRESSION OF FGF-5 AND FGF-7 IN THE CYCLOSPORIN A-INDUCED GINGIVAL HYPERPLASIA

Cyclosporin A에 의한 치은 증식증에서 FGF-5와 FGF-7의 발현 양상에 대한 연구

  • Jeong, Mi-Hyang (Dept. of Orthodontics, College of Dentistry, Seoul National University) ;
  • Kim, Seong-Gon (Dept. of Oral and Maxillofacial Surgery, College of Medicine, Hallym University) ;
  • Yun, Kyoung-In (Dept. of Oral and Maxillofacial Surgery, College of Medicine, Eulji University) ;
  • Nahm, Dong-Seok (Dept. of Orthodontics, College of Dentistry, Seoul National University)
  • 정미향 (서울대학교 치과대학 치과교정과) ;
  • 김성곤 (한림대학교 의과대학 성심병원 구강악안면외과) ;
  • 윤경인 (을지의과대학교 을지병원 구강악안면외과) ;
  • 남동석 (서울대학교 치과대학 치과교정과)
  • Published : 2006.06.30

Abstract

Cyclosporin A-induced gingival hyperplasia is frequently found in the patients who have been received an immunosuppressant for the organ transplantation. However, its exact mechanism is still unknown. The expression of FGF-5 and FGF-7 were studied in cyclosporine A-induced gingival hyperplasia (CGH) and inflammatory gingival hyperplasia (IGH). Immunohistochemistry and in situ hybridization were used for localization of protein and mRNA. The expression of FGF-5 and FGF-7 was different from CGH and IGH. FGF-5 and FGF-7 was strongly expressed in fibroblast in CGH (P<0.005 and P<0.05, respectively). FGF-5 mRNA was localized in the middle portion of connective tissue. FGF-7 mRNA was also identified in fibroblasts and mast cells. In conclusion, FGF-5 and FGF-7 were produced excessively by fibroblasts in CGH. Considering their known functions, their expression in CGH is important for production of collagen and proliferation of fibroblasts.

Keywords

References

  1. Abbas AK, Lichtman AH, Pober JS: Cellular and molecular immunology. W.B.Saunders company. 1991:165
  2. Kronke M, Leonard WJ, Depper JM, Arya SK, Wong-Staal F, Gallo RC, et al: Cyclosporine A inhibits T-cell growth factor gene expression at the level of mRNA transcription. Proc Natl Acad Sci USA 1984;81:5214-5218
  3. Shevach EM: The effects of cyclosporin A on the immune system. Ann Rev Immunol 1985;3:397-423 https://doi.org/10.1146/annurev.iy.03.040185.002145
  4. Partridge M, Kiguwa S, Luqmani Y, Langdon JD: Expression of bFGF, KGF and FGF receptors on normal oral mucosa and SCC. Eur J Cancer B Oral Oncol 1996;32B:76-82
  5. Garrone P, Neidhardt EM, Garcia E, Galibert L, van Kooten C, Banchereau J: Fas ligation induces apoptosis of CD40-activated human B lymphocytes. J Exp Med 1995;182:1265-1273 https://doi.org/10.1084/jem.182.5.1265
  6. Kong YY, Yoshida H, Sarosi I, Tan HL, Timms E, Capparelli C, et al: OPGL is a key regulator of osteoclastogenesis, lymphocyte development and lymph-node organogenesis. Nature 1999;397:315-323 https://doi.org/10.1038/16852
  7. Mariani G, Calastrini C, Carinci F, Bergamini L, Calastrini F, Stabellini G: Ultrastructural and histochemical features of the ground substance in cyclosporon A induced gingival hyperplasia. J Periodontol 1996;67:21-27 https://doi.org/10.1902/jop.1996.67.1.21
  8. Clase KL, Mitchell PJ, Ward PJ, Dorman CM, Johnson SE, Hannon K: FGF-5 stimulates expansion of connective tissue fibroblast and inhibits skeletal muscle development in the limb. Dev Dyn 2000; 219: 368-380 https://doi.org/10.1002/1097-0177(2000)9999:9999<::AID-DVDY1056>3.0.CO;2-8
  9. Werner S, Peters KG, Longaker MT, Fuller-Pace F, Banda MJ, Williams LT: Large induction of keratinocyte growth factor expression in the dermis during wound healing. Proc Natl Acad Sci USA 1992; 89: 6896-6900
  10. Asahara Y, Nishimura F, Yamada H, Naruishi K, Kataoka M, Kido J, et al: Mast cell are not involved in the development of cyclosporin Ainduced gingival hyperplasia : A study with mast cell-deficient mice. J Periodontol 1990; 71: 1117-1120 https://doi.org/10.1902/jop.2000.71.7.1117
  11. Berton A, Levi-Schaffer F, Emonard H, Garbuzenko E, Gillery P, Maquart FX: Activation of fibroblast in collagen lattices by mast cell extract : a model of fibrosis. Clin Exp Allergy 2000; 30: 485-492 https://doi.org/10.1046/j.1365-2222.2000.00737.x
  12. Ortiz A, Lorz C, Gonzalez-Cuadrado S, Garcia del Moral R, O'Valle F, Egido J: Cytokines and Fas regulate apoptosis in murine renal interstitial fibroblast. J Am Soc Nephrol 1997; 8: 1845-1854
  13. Garrone P, Neidhardt E, Garcia E, Galibert L, van Kooten C, Banchereau J: Fas ligation induces apoptosis of CD40-activated human B lymphocytes. J Exp Med 1995; 182: 1265-1273 https://doi.org/10.1084/jem.182.5.1265
  14. Jelaska A, Arakawa M, Broketa G, Korn JH: Heterogeneity of collagen synthesis in normal and systemic sclerosis skin fibroblasts. Increased proportion of high collagen-producing cells in systemic sclerosis fibroblasts. Arthritis Rheum 1996; 39: 1338-1346 https://doi.org/10.1002/art.1780390811
  15. Wolf G, Thaiss F, Stahl RA: Cyclosporine stimulates expression of transforming growth factor-beta in renal cells. Possible mechanism of cyclosporines proliferative effects. Transplantation 1995; 60: 237-241 https://doi.org/10.1097/00007890-199508000-00005
  16. Bartold PM: Regulation of human gingival fibroblast growth and synthetic activity by cyclosporin-A in vitro. J Periodont Res 1989; 24: 314-321 https://doi.org/10.1111/j.1600-0765.1989.tb00876.x