Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Solubility, Antioxidative and Antimicrobial Activity of Chitosan-Ascorbate

키토산-아스코베이트의 용해성, 항산화성 및 항균성

  • Lee, Seung-Bae (Dept. of Food Science and Technology, Food Industrial Technology, Catholic University of Daegu) ;
  • Lee, Ye-Kyung (Dept. of Food Science and Technology, Food Industrial Technology, Catholic University of Daegu) ;
  • Kim, Soon-Dong (Dept. of Food Science and Technology, Food Industrial Technology, Catholic University of Daegu)
  • 이승배 (대구가톨릭대학교 식품외식산업학부 식품공학) ;
  • 이예경 (대구가톨릭대학교 식품외식산업학부 식품공학) ;
  • 김순동 (대구가톨릭대학교 식품외식산업학부 식품공학)
  • Published : 2006.08.30
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Abstract

This study was conducted to investigate the solubility, antioxidative and antimicrobial activity of the freeze dried chitosan-ascorbate (CAs) and chitosan-acetate (CAc). In the results of solubility, CAs was soluble over 0.5% in distilled water, vinegar, green tea, soju (distilled liquor), beer and red wine, while it was not soluble in soy sauce, soy milk, milk, orange juice, coffee, sesame oil, soy milk and soybean oil. The solubility of CAc in the liquid foods was similar to those of CAs, but it was soluble less than 0.1% in beer, and formed curd in red wine. Electron donating activity, antioxidative activity and SOD activity of CAs were 48.2, 90.6 and 67.5%, respectively, while the activities of the CAc were 0, 40.0 and 10.0%, respectively. The minimal inhibitory concentrations of CAs and CAc were $200\;{\mu}g/disc$ against Bacillus circulans, Bacillus brevis, Bacillus licheniformis, Bacillus arabitane and Bacillus sterothermophillus, $400\;{\mu}g/disc$ against Escherichia coli O157, Listeria monocytogenous, Bacillus cereus and Bacillus subtilis. There was no significant difference in Hunter's L* value between CAs and CAc $(81.95{\sim}82.97)$, but Hunter's a* and b* values of the CAs was higher than those of CAc. While sour and bitter tastes of CAs were lower than those of CAc, there was no significant difference in astringent taste. From these results, it suggested that CAs has more extensive utility in liquid foods with antimicrobial and antioxidant activity as well as sensory quality compared to CAc.

동결건조한 chitosan-ascorbate(CAs)와 chitosan-acetate(CAc)의 용해성, 항균성, 항산화성을 비교하였다. 용해성을 조사한 결과 CAs는 증류수, 식초, 녹차, 소주, 맥주, 적포도주에 0.5%이상의 농도로 용해되었으나 간장, 두유, 우유, 오렌지주스, 커피, 참기름, 대두유에는 녹지 않았다. CAc도 CAs의 경우와 비슷하나 맥주에서는 0.1%이하의 농도에서 용해되었으며 적포도주에서는 커드를 형성하였다. CAc의 전자공여능, 항산화능 및 SOD활성은 CAc에서는 각각 0, 40.0 및 10.0%였으나 CAs의 경우는 48.2, 90.6 및 67.5%로 CAc보다 높은 활성을 나타내었다. B. circulans, B. brevis, B. licheniformis, B. arabitane, B. sterothermophillus에 대한 CAs와 CAc의 최소저해농도(MIC)는 다같이 $200\;{\mu}g/disc$이었으며, E. coli O157, Listeria monocytogenous, B. cereus, B. subtilis에 대한 MIC는 다같이 $400\;{\mu}g/disc$으로 뚜렷한 차이를 보이지 않았다. CAs와 CAc의 Hunter's L*값은 $81.95{\sim}82.97$로 뚜렷한 차이가 없었으나 Hunter's a* 및 b*값은 CAs가 높았다. 관능검사 결과, CAs는 CAc에 비하여 신맛과 쓴맛은 낮았으나 떫은맛은 뚜렷한 차이가 없었다. 결론적으로 CAs는 CAc에 비하여 항균성, 항산화성 및 기호도 측면에서 우수하여 식품에의 활용이 기대된다.

Keywords

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