Journal of Acupuncture Research
- Volume 23 Issue 5
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- Pages.187-198
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- 2006
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- 2586-288X(pISSN)
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- 2586-2898(eISSN)
Effect of Daekumeumja Herb-acupuncture on Alcohol-induced Suppressed Cell Proliferation and Expression of Nitric Oxide Synthase in Hippocampus of Rats
대금음자(對金飮子) 약침이 알코올 독성 흰쥐의 해마에서 신경세포생성과 NOS 발현에 미치는 영향
- Kim, Hyun-Joong (Department of Acupuncture & Moxibustion, College of Oriental Medicine, Se-Myung University) ;
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Kim, Ee-Hwa
(Department of Meridianology, College of Oriental Medicine, Se-Myung University) ;
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Lee, Eun-Yong
(Department of Acupuncture & Moxibustion, College of Oriental Medicine, Se-Myung University)
- Published : 2006.10.20
Abstract
Objectives : The purpose of this study is to assess the effect of Daekumeumja herb-acupuncture on neural cell proliferation and nitric oxide synthase(NOS) expression in hippocampus of ethanol-intoxicated Sprague-Dawley(SD) rats. Methods : SD rats were randomly assigned into 5 groups ; the normal group, the alcohol-treated(control) group, the alcohol- and 1 mg/kg Daekumeumja- treated(sample A) group, the alcohol- and 5mg/kg Daekumeumja-treated (sample B) group, the alcohol- and 10 mg/kg Daekumeumja-treated (sample C) group(n = 6 in each group). Normal group were received with Saline, while control group were injected intraperitoneally with alcohol(2 g/kg) once per day for 5 days. Sample groups were treated Daekumeumja herb-acupuncture on Chungwan(CV12) for 5 consecutive days. Bromo-deoxyuridine(BrdU) was injected into all animals once per day for 5 days. For the detection of BrdU-positive cells and NADPH-d- positive cells in hippocampus, immunohistochemistry was performed. Results : 1. In the dentate gyrus area, the number of BrdU-positive cells in the sample