Journal of Acupuncture Research
- Volume 23 Issue 5
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- Pages.199-206
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- 2006
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- 2586-288X(pISSN)
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- 2586-2898(eISSN)
Effect of Electroacupuncture Stimulation on Activity of Neuronal NOS in Rats
흰쥐의 neuronal NOS 신경세포의 activity에 대한 전침자극 효과
- Kim, Hoo-Dong (Department of Acupuncture & Moxibustion, Oriental Medical Hospital, Kyung Hee University) ;
- Nam, Sang-Soo (Department of Acupuncture & Moxibustion, Kangnam Kyung-Hee Korean Hospital, Kyung-Hee University) ;
- Kim, Chang-Hwan (Department of Acupuncture & Moxibustion, Oriental Medical Hospital, Kyung Hee University)
- Published : 2006.10.20
Abstract
Objectives : The aim of this study was to investigate the effect of various electroacupuncture stimulation on neuronal nitric oxide synthase(nNOS) in cerebral cortex, brain stem, cerebellum of spontaneously hypertensive rats. Methods : We evaluated the changes of nNOS-positive neurons using a immunohistochemical method. The staining intensity of nNOS positive neurons was assessed in a quantitative fashion using a microdensitometrical method based on optical density by means of an image analyzer. Results : The average optical density of nNOS-positive neurons of 100 Hz (bipolar square wave 0.2 ms duration and 100 Hz frequency) electroacupuncture treatment group significantly decreased in most cortical areas comparison between the manual acupuncture and 2 Hz (bipolar square wave 0.2 ms duration and 2 Hz frequency) electroacupuncture groups. In the brain stem, the optical density of nNOS-positive neuron at superficial gray layer of the superior colliculus area, dorsolateral periaqueductal gray area and paralemniscal nucleus were same as cerebral cortex. Conclusion : We conclude that the morphological evidence for nNOS-positive neurons may be have regional change in cerebral cortex brain stem and cerebellum according to various electroacupuncture stimulations.
Keywords
- Electroacupuncture stimulations;
- neuronal nitric oxide synthase(nNOS);
- Cerebral cortex;
- Brain stem;
- Cerebellum