Korean Journal of Soil Science and Fertilizer (한국토양비료학회지)

Korean Society of Soil Science and Fertilizer (한국토양비료학회)
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Isolation of Sulfur Oxidizing Bacteria from Different Ecological Niches

다양한 생태계에서 분리한 황 산화 세균

  • Anandham, Rangasamy (Department of Agricultural Microbiology, Tamil nadu Agricultural University) ;
  • Sridar, Ragupathy (Department of Agricultural Microbiology, Tamil nadu Agricultural University) ;
  • Nalayini, Periyakaruppan (Division of Crop Production, Central Institute for Cotton Research, Regional Station, Indian Council Agricultural Research) ;
  • Madhaiyan, Munusamy (Department of Agricultural Chemistry, Chungbuk National University) ;
  • Gandhi, Pandiyan Indira (Department of Agricultural Chemistry, Chungbuk National University) ;
  • Choi, Kwan-Ho (Department of Agricultural Chemistry, Chungbuk National University) ;
  • Sa, Tong-Min (Department of Agricultural Chemistry, Chungbuk National University)
  • ;
  • ;
  • ;
  • ;
  • ;
  • 최관호 (충북대학교 농업생명환경대학 농화학과) ;
  • 사동민 (충북대학교 농업생명환경대학 농화학과)
  • Received : 2005.07.02
  • Accepted : 2005.07.28
  • Published : 2005.08.30
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Abstract

Nine chemolithoautotrophic and 12 chemolithoheterotrophic sulfur oxidizing bacteria were isolated using enrichment technique in modified Starkey's medium. All isolates reduced pH of the growth medium through oxidation of elementai sulfur to sulfuric acid. Isolates utilized the thiosulfate as energy source except LCH. None of the isolates grew anaerobically and utilization of glucose was found only in chemolithoheterotrophic isolates SGA6 and JIG. In vitro sulfate production from elemental sulfur was found maximum for chemoiithoautotroph LCH ($43.2mg\;100\;mL^{-1}$) and least for chemolithoheterotroph JIG ($10.04mg\;100\;mL^{-1}$). The above tests suggested that all isolates belong to the member of Thiobacillus. For field inoculation of Thiobacillus, clay based pellet formulation was developed with the cell load of $2.5{\times}10^7cfu\;g^{-1}$ of pellet. It is easy to handle by the farmers and more likely to lead to successful farming.

다양한 인도 토양에서 Starkey's 배지를 이용하여 9종류의 화학합성독립영양 및 12종류의 화학합성종속영양 황산화 세균을 분리하였다. 분리한 세균은 황을 황산으로 산화시키는 기능이 있으며 생육 중 배지의 산도를 감소시켰다. 분리한 황 산화 세균은 혐기 조건에서는 생육하지 못했다. 화학합성독립영양세균 SGA6 및 JIG 2종만이 포도당을 이용하는 능력이 있었으며, LCH 1종을 제외한 모든 분리세균은 thiosulfate를 에너지원으로 이용하였다. 분리한 세균 중 황 산화 능력이 가장 우수한 것은 화학합성독립영향세균인 LCH이었으며 화학합성종속영향세균인 JIG의 황 산화 능력이 가장 낮았다. 분리한 황 산화 세균을 이용하여 점토를 이용하여 입제형태로 만들었을 때 균의 밀도는 약 $2.5{\times}10^7cfu\;g^{-1}$ 이었다.

Keywords

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